Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
836,005 |
T→A |
23.0% |
S241S (TCT→TCA) |
narH → |
nitrate reductase 1, beta (Fe‑S) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 836,005 | 0 | T | A | 23.0%
| 145.7
/ 43.7
| 74 | S241S (TCT→TCA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/39); new base A (4/13); total (22/52) |
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 |
GTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCG > minE/835935‑836075
|
gTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt < 1:239832/71‑1 (MQ=255)
tggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTg < 1:2128510/71‑1 (MQ=255)
ggctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCt > 1:2222021/1‑69 (MQ=255)
gctggcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:203394/71‑1 (MQ=255)
tggcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAgaaa > 1:1152407/1‑68 (MQ=255)
gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat > 1:1719179/1‑69 (MQ=255)
gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3241472/69‑1 (MQ=255)
gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:3246154/69‑1 (MQ=255)
gcGTTTGTGCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaa < 1:1843920/69‑1 (MQ=255)
gcGTATGTTCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAg > 1:2915481/1‑70 (MQ=255)
gcGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGt > 1:3747510/1‑71 (MQ=255)
cGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgag < 1:934189/66‑1 (MQ=255)
tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1503231/71‑1 (MQ=255)
tATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCa < 1:1710470/71‑1 (MQ=255)
aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:1890133/1‑71 (MQ=255)
aTGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:309516/1‑71 (MQ=255)
tgtgCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtc < 1:1561817/71‑1 (MQ=255)
tgtgCATAAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCAtc > 1:778851/1‑71 (MQ=255)
cATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:1723363/70‑1 (MQ=255)
tCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGc > 1:241643/1‑71 (MQ=255)
aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:2942732/71‑1 (MQ=255)
aCTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTa < 1:501911/71‑1 (MQ=255)
cTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTAt > 1:3086932/1‑71 (MQ=255)
ggATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATcc > 1:2096601/1‑71 (MQ=255)
aTGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGaa < 1:2546401/51‑1 (MQ=255)
tGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTga < 1:136854/47‑1 (MQ=255)
tccGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCcacgaa < 1:2509527/70‑3 (MQ=255)
cGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTAtt < 1:1497057/71‑1 (MQ=255)
gTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:960377/71‑1 (MQ=255)
tACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTgaga < 1:1231986/43‑1 (MQ=255)
cAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAt > 1:2328939/1‑48 (MQ=255)
cAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGag < 1:1155359/70‑2 (MQ=255)
aaaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtcttc < 1:3307498/50‑1 (MQ=255)
aaaaaaTCTACTTCAACTGGAAAAGCGGCAAGTCAGaaaaat < 1:2405111/42‑3 (MQ=255)
aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTa < 1:3062759/62‑1 (MQ=255)
aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTg < 1:1252982/65‑1 (MQ=255)
aaaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCCTCTGCTATCCGCGTAtt < 1:299188/64‑1 (MQ=255)
aaaaTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTATGCTATCCGCGTATTGAAGCggg < 1:3819574/71‑1 (MQ=255)
aTCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCa < 1:3498115/71‑1 (MQ=255)
tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:3554602/67‑1 (MQ=255)
tCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCggg < 1:2545390/67‑1 (MQ=255)
tCTACTTCAACTGGAAGAGAGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAg > 1:3360921/1‑63 (MQ=255)
aCTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCAtctt < 1:1433275/40‑1 (MQ=255)
cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:2234600/69‑1 (MQ=255)
cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGcc < 1:3729428/69‑1 (MQ=255)
cTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCg < 1:1050029/70‑1 (MQ=255)
cTTCAACTCCAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAACCGGGTCAGcc < 1:224040/69‑1 (MQ=255)
cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:1174327/1‑71 (MQ=255)
cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:2590286/1‑71 (MQ=255)
cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg > 1:39260/1‑71 (MQ=255)
aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:514625/71‑1 (MQ=255)
aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgt < 1:1229407/71‑1 (MQ=255)
aCTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTac < 1:611707/41‑2 (MQ=12)
tGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGtgtg > 1:2997512/1‑71 (MQ=255)
gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCt < 1:534380/71‑1 (MQ=255)
gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:364190/45‑1 (MQ=255)
gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGa < 1:359474/45‑1 (MQ=255)
gagCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAg < 1:431094/71‑1 (MQ=255)
agCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAg > 1:2151562/1‑70 (MQ=255)
agCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGa < 1:78065/71‑1 (MQ=255)
gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg < 1:1834760/48‑1 (MQ=255)
gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGa < 1:2602887/70‑1 (MQ=255)
cGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCgtgt < 1:2625560/62‑1 (MQ=255)
caaGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTc < 1:1988407/62‑1 (MQ=255)
aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:1747970/1‑43 (MQ=255)
aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCgg > 1:2661141/1‑43 (MQ=255)
aaGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCt < 1:730330/70‑1 (MQ=255)
aaGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtg < 1:1467286/71‑1 (MQ=255)
aGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgt > 1:1183589/1‑71 (MQ=255)
aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:1451602/71‑2 (MQ=255)
aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:57487/71‑2 (MQ=255)
aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc < 1:1001021/71‑2 (MQ=255)
gTCTGAGAAGTTCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgtg < 1:3809722/71‑1 (MQ=255)
tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgtg < 1:3616713/70‑1 (MQ=255)
cTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtc > 1:1855850/1‑71 (MQ=255)
cTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtc > 1:2418464/1‑71 (MQ=255)
tGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcg > 1:713415/1‑71 (MQ=255)
|
GTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCG > minE/835935‑836075
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
