Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,169 |
C→A |
26.9% |
V300V (GTG→GTT) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,169 | 0 | C | A | 26.9%
| 190.0
/ 47.7
| 89 | V300V (GTG→GTT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (31/34); new base A (11/13); total (42/47) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
ATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAT > minE/1015100‑1015237
|
atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:2950878/1‑71 (MQ=255)
atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:683627/1‑71 (MQ=255)
tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcg > 1:67741/1‑71 (MQ=255)
tACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAc > 1:1604791/1‑70 (MQ=255)
cGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc < 1:2385778/70‑1 (MQ=255)
gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:3702762/69‑1 (MQ=255)
tCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAAt < 1:458145/71‑1 (MQ=255)
agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGt > 1:1140145/1‑71 (MQ=255)
gcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc > 1:613430/1‑71 (MQ=255)
agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:3294539/71‑1 (MQ=255)
gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt > 1:3061506/1‑70 (MQ=255)
gagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:2411039/71‑1 (MQ=255)
agcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTg < 1:1499837/70‑1 (MQ=255)
cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg < 1:949085/70‑1 (MQ=255)
agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCgg < 1:2169736/59‑1 (MQ=255)
agGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1398604/71‑1 (MQ=255)
ggAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:3311337/69‑1 (MQ=255)
gagaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGaa < 1:1268342/71‑1 (MQ=255)
gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:1711353/69‑1 (MQ=255)
gagaCGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:2058641/69‑1 (MQ=255)
agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:677158/71‑1 (MQ=255)
agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:3228572/71‑1 (MQ=255)
gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:1438358/1‑70 (MQ=255)
gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:964574/1‑70 (MQ=255)
aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:1438279/1‑71 (MQ=255)
aCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTa > 1:6168/1‑71 (MQ=255)
cccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:3245503/70‑1 (MQ=255)
ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:717175/1‑71 (MQ=255)
ccATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:747980/1‑71 (MQ=255)
cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:3696802/71‑1 (MQ=255)
atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:3217624/4‑71 (MQ=255)
atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:242734/4‑71 (MQ=255)
tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAg > 1:2835182/1‑57 (MQ=255)
tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:1057730/1‑71 (MQ=255)
gccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGa < 1:3117168/58‑1 (MQ=255)
accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:1268200/1‑71 (MQ=255)
accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:3530167/1‑71 (MQ=255)
accgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2071780/1‑70 (MQ=255)
ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccac > 1:2396244/1‑70 (MQ=255)
cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc > 1:2415730/1‑70 (MQ=255)
gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca > 1:401388/1‑70 (MQ=255)
accgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCg < 1:3857586/54‑1 (MQ=255)
accgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:1992963/1‑71 (MQ=255)
cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:3477962/71‑1 (MQ=255)
cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:577221/71‑1 (MQ=255)
cgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2441658/71‑1 (MQ=255)
gggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2890421/71‑1 (MQ=255)
ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:2087469/1‑62 (MQ=255)
ggCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:705830/1‑62 (MQ=255)
ggCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgc < 1:1629995/71‑1 (MQ=255)
aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:2946039/54‑1 (MQ=255)
aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:267602/54‑1 (MQ=255)
aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa > 1:2787290/1‑71 (MQ=255)
aGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgcgcg > 1:2597170/1‑70 (MQ=255)
aTGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTc < 1:1061449/37‑1 (MQ=255)
tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCa < 1:2837449/71‑1 (MQ=255)
cgcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGa > 1:2380973/1‑71 (MQ=255)
gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:3144837/43‑1 (MQ=255)
gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:2926827/43‑1 (MQ=255)
gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:562541/43‑1 (MQ=255)
gcCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAg < 1:691562/43‑1 (MQ=255)
ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCa < 1:472444/63‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:36690/46‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:2301721/46‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2259648/71‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:322860/71‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc > 1:3107929/1‑70 (MQ=255)
cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:73788/1‑70 (MQ=255)
cGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAcc > 1:2369732/1‑70 (MQ=255)
cGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2094105/70‑1 (MQ=255)
tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt > 1:186372/1‑55 (MQ=255)
gACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTaccacc < 1:3492478/49‑1 (MQ=255)
aCGTGGCAGGCGAATCACGAGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:117988/1‑52 (MQ=255)
gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt > 1:1204794/1‑71 (MQ=255)
tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:3538794/71‑3 (MQ=255)
ggCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg > 1:3915066/1‑50 (MQ=255)
gCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:3942081/36‑1 (MQ=255)
gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:3748278/1‑70 (MQ=255)
gCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgg > 1:197621/1‑70 (MQ=255)
aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:2654428/68‑1 (MQ=255)
aGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTg < 1:1729927/68‑1 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3176725/1‑51 (MQ=255)
gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACt < 1:2200366/42‑1 (MQ=255)
gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:2672173/49‑1 (MQ=255)
aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCaaa > 1:404564/1‑39 (MQ=255)
aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGa < 1:2636938/71‑1 (MQ=255)
aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCGTCGa < 1:1510792/71‑1 (MQ=255)
aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:2149742/1‑71 (MQ=255)
tCACGCGGATGTCGTGAGCGAAGTATTTACCACCTAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1224255/1‑70 (MQ=255)
|
ATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAT > minE/1015100‑1015237
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
