Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,182 |
G→C |
11.7% |
T87T (ACC→ACG) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,182 | 0 | G | C | 11.7%
| 252.4
/ 19.8
| 94 | T87T (ACC→ACG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (41/42); new base C (5/6); total (46/48) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 |
CGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCA > minE/1934112‑1934252
|
cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:605132/1‑71 (MQ=255)
ttATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt < 1:3138423/71‑1 (MQ=255)
tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:84639/1‑70 (MQ=255)
ataAACGCCTTCCGGGAGATTGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:1146836/1‑71 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1110733/71‑1 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1251231/71‑1 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:414129/71‑1 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2757308/1‑70 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:3482992/1‑69 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:1195468/1‑69 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2351674/1‑69 (MQ=255)
cTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc > 1:3822468/1‑71 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGTATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:1339389/70‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:1693437/69‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:1586997/1‑71 (MQ=255)
gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3897061/61‑1 (MQ=255)
gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt > 1:2888995/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:1706607/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:3931835/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAAAGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:436605/71‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2413681/70‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATACGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa > 1:3835260/1‑71 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:3817657/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1682024/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1893285/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:46477/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3872507/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:654561/71‑1 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:2058786/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1019782/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1667779/1‑70 (MQ=255)
gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1479895/1‑67 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:2661970/46‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:2197201/52‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2383178/1‑70 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:874430/1‑70 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3682362/1‑52 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3636070/1‑52 (MQ=255)
aCGTGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1721183/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTa > 1:801775/1‑46 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa > 1:314147/1‑52 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3568748/71‑1 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2946840/71‑1 (MQ=255)
cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:1342083/4‑70 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata > 1:2664461/1‑65 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATTATacga < 1:3212337/71‑1 (MQ=255)
gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAAc > 1:373295/1‑44 (MQ=255)
gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg < 1:1001202/71‑1 (MQ=255)
cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1176573/47‑1 (MQ=255)
cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc < 1:3945102/71‑1 (MQ=255)
cGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:2175408/1‑50 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:1685103/1‑46 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2562071/1‑70 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:3091605/1‑70 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:146099/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1020139/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1719053/70‑1 (MQ=255)
aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCTATAATACGACGGGTcatcat < 1:1842042/71‑1 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:2098882/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:1474238/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcat < 1:3442194/68‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACggg < 1:2773660/62‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3422759/70‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3424822/70‑1 (MQ=255)
tGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:2419705/58‑1 (MQ=255)
gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:613890/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2435959/1‑71 (MQ=255)
aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg < 1:1966525/70‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:1549006/70‑1 (MQ=255)
aTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc < 1:3845564/70‑1 (MQ=255)
aCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:1676818/71‑1 (MQ=255)
cGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:3731146/71‑1 (MQ=255)
gcaaaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1476048/4‑71 (MQ=255)
gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAAgg > 1:1291757/1‑71 (MQ=255)
gCCAACGTCCTGCA‑GGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:1437688/71‑1 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata < 1:3426055/42‑1 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:3403473/1‑71 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:2043395/71‑1 (MQ=255)
aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa > 1:1720525/1‑61 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:1275403/1‑39 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3071995/1‑55 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:2494688/1‑55 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGATTTACCCataata > 1:879284/1‑39 (MQ=255)
tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2141904/71‑1 (MQ=255)
tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2322979/71‑1 (MQ=255)
tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:2844694/71‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:2069430/70‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1274658/70‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa < 1:2120878/71‑1 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3842180/70‑2 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:1776962/70‑2 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACAGAGACTTcaa > 1:1040718/1‑69 (MQ=255)
tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt < 1:1167715/67‑1 (MQ=255)
cAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat > 1:327039/1‑45 (MQ=255)
aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:598419/1‑71 (MQ=255)
aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:258544/1‑71 (MQ=255)
ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:3160277/71‑1 (MQ=255)
|
CGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCA > minE/1934112‑1934252
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
