Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,076,873 |
C→T |
21.3% |
H147Y (CAT→TAT) |
ebgC → |
cryptic beta‑D‑galactosidase, beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,076,873 | 0 | C | T | 21.3%
| 92.8
/ 19.4
| 47 | H147Y (CAT→TAT) | ebgC | cryptic beta‑D‑galactosidase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (20/17); new base T (3/7); total (23/24) |
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACG > minE/2076806‑2076942
|
tCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCat > 1:2615147/1‑70 (MQ=255)
gTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataaca < 1:643099/71‑1 (MQ=255)
tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaata < 1:56183/70‑1 (MQ=255)
tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataa > 1:1603395/1‑71 (MQ=255)
tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaata < 1:2101030/70‑1 (MQ=255)
tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaata < 1:21084/70‑1 (MQ=255)
tCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaata < 1:2159013/70‑1 (MQ=255)
aaCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac > 1:1072828/1‑67 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac > 1:3531915/1‑66 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac > 1:3527372/1‑66 (MQ=255)
aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcgg > 1:2526387/1‑71 (MQ=255)
gTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCa < 1:905060/71‑1 (MQ=255)
cAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa > 1:2142926/1‑70 (MQ=255)
aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataa > 1:958947/1‑54 (MQ=255)
aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAg < 1:1042732/71‑1 (MQ=255)
aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAg > 1:1909046/1‑71 (MQ=255)
aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCat > 1:1922547/1‑43 (MQ=255)
aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCat > 1:1063123/1‑43 (MQ=255)
aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:2580548/71‑1 (MQ=255)
aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg > 1:564180/1‑71 (MQ=255)
aGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGt < 1:3198806/71‑1 (MQ=255)
gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGtt < 1:2639232/71‑1 (MQ=255)
tGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaata < 1:457978/46‑1 (MQ=255)
tGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaata < 1:2734507/46‑1 (MQ=255)
tGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaaaa > 1:352882/1‑64 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCTataacaa < 1:3734688/38‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:1696399/43‑1 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:872234/1‑71 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:2381174/1‑71 (MQ=255)
ctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccg < 1:1362710/70‑1 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg < 1:2713038/48‑1 (MQ=255)
aaaGTCACCATCGAAGATGGTTATTTCCATAACAAAT‑ACAACTACGGCGGCAAAAGGAGTTTGCCGCCAc < 1:3898771/70‑1 (MQ=255)
aaGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAcc > 1:3880586/1‑71 (MQ=255)
aaGTCACCATCGAAGATGGTTATTTCCATAACAAATACCACCTAcggcgg > 1:3882316/1‑50 (MQ=255)
aaGCCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAcc > 1:3764629/1‑71 (MQ=255)
ccATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGa < 1:1012022/52‑1 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTAcgg > 1:3096960/1‑36 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACt > 1:184178/1‑71 (MQ=255)
gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACt > 1:2423348/1‑71 (MQ=255)
aaGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACt > 1:320938/1‑70 (MQ=255)
tGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAtt < 1:3587979/70‑1 (MQ=255)
ggTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCAttt > 1:2552671/1‑70 (MQ=255)
ttATTTCCATAACAAATAACAACTACGGCGGCaaa < 1:3568200/35‑1 (MQ=255)
ttATTTCCATAACAAATAACAACTACGGCGGCaaa < 1:3151313/35‑1 (MQ=255)
tATTTCCATAACAAATAACAACTACGGCGGCAAAAgg < 1:1396458/37‑1 (MQ=255)
tATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg < 1:2779604/70‑1 (MQ=255)
ttCTATAACAAATAACAACTACGGCGGCAAAAGGa < 1:3236067/35‑1 (MQ=255)
ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACg > 1:2551321/1‑71 (MQ=255)
|
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACG > minE/2076806‑2076942
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
