Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I0 R1	2169	63.0	3952800	76.2%	3012033	62.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,130,629	G→A	9.8%	G92G (GGC→GGT)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,130,629	0	G	A	9.8%	294.4 / 15.9	102	G92G (GGC→GGT)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand): ref base G (37/55); new base A (6/4); total (43/59)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGA  >  minE/2130560‑2130693

aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                                 >  1:3756300/1‑71 (MQ=255)

   cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                                                               <  1:3419028/70‑1 (MQ=255)

   cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                                                               <  1:313305/70‑1 (MQ=255)

   cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCg                                                               <  1:2476653/70‑1 (MQ=255)

    aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             >  1:3841630/1‑71 (MQ=255)

     gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             >  1:3882045/1‑70 (MQ=255)

     gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg                                                            <  1:3419420/71‑1 (MQ=255)

       aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa                                                           >  1:2638131/1‑70 (MQ=255)

       aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                                                              <  1:2570390/67‑1 (MQ=255)

       aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                                                              <  1:220924/67‑1 (MQ=255)

        tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                          >  1:1333389/1‑70 (MQ=255)

        tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                          >  1:2752857/1‑70 (MQ=255)

          gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc                                                       >  1:431476/1‑71 (MQ=255)

          gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc                                                       >  1:3038275/1‑71 (MQ=255)

            aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                                     <  1:1730270/71‑1 (MQ=255)

            aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                                     <  1:2963379/71‑1 (MQ=255)

             ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                                 >  1:1487521/1‑58 (MQ=255)

              cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             <  1:645678/61‑1 (MQ=255)

              cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             <  1:2286262/61‑1 (MQ=255)

                 tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg                                                >  1:1530889/1‑71 (MQ=255)

                   gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAggt                                              <  1:2258372/71‑3 (MQ=255)

                    cAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGataa                                                        <  1:2207693/60‑4 (MQ=255)

                    cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                             <  1:3900250/71‑1 (MQ=255)

                    cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                             <  1:631903/71‑1 (MQ=255)

                     aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             <  1:315029/54‑1 (MQ=255)

                     aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                                   <  1:1768982/64‑1 (MQ=255)

                     aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCtt                                            <  1:3786149/71‑1 (MQ=255)

                     aGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                             <  1:476426/70‑1 (MQ=255)

                       aaaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTg                                          <  1:2108386/71‑1 (MQ=255)

                          tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                        <  1:1300912/70‑1 (MQ=255)

                            taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCa                                      <  1:205213/70‑1 (MQ=255)

                             aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTg                                                            <  1:159205/47‑1 (MQ=255)

                              ggCATACGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             <  1:267439/45‑1 (MQ=255)

                               gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                  <  1:2968351/71‑1 (MQ=255)

                                 aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                    <  1:3927449/67‑1 (MQ=255)

                                 aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                <  1:572894/71‑1 (MQ=255)

                                  tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                               >  1:79700/1‑71 (MQ=255)

                                   aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              >  1:680087/1‑71 (MQ=255)

                                   aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              >  1:342043/1‑71 (MQ=255)

                                    ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             <  1:2775292/71‑1 (MQ=255)

                                    ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              <  1:1548219/70‑1 (MQ=255)

                                     gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            <  1:2810825/71‑1 (MQ=255)

                                     gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            <  1:2512851/71‑1 (MQ=255)

                                      tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           <  1:2372374/71‑1 (MQ=255)

                                        aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               <  1:596894/49‑1 (MQ=255)

                                        aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               <  1:2374901/49‑1 (MQ=255)

                                        aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               <  1:2156590/49‑1 (MQ=255)

                                         gtatatatAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                 <  1:769920/60‑1 (MQ=255)

                                          gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               <  1:1485245/47‑1 (MQ=255)

                                           atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      >  1:1591118/1‑71 (MQ=255)

                                           atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      >  1:3774950/1‑71 (MQ=255)

                                           atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      <  1:3234720/71‑1 (MQ=255)

                                            tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                  <  1:3151871/58‑1 (MQ=255)

                                             ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg                    >  1:2353629/1‑71 (MQ=255)

                                             ataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATgg                    >  1:398382/1‑71 (MQ=255)

                                              taGAGCACTAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   <  1:177020/71‑1 (MQ=255)

                                              taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg                    <  1:3634079/70‑1 (MQ=255)

                                              taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   >  1:1037977/1‑71 (MQ=255)

                                              taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   >  1:1700784/1‑71 (MQ=255)

                                              taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   <  1:2526857/71‑1 (MQ=255)

                                              taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt                   <  1:1728274/71‑1 (MQ=255)

                                               agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   >  1:378976/1‑70 (MQ=255)

                                                gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg                  <  1:2954836/70‑1 (MQ=255)

                                                  gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc                <  1:2107454/70‑1 (MQ=255)

                                                   cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      <  1:58497/63‑1 (MQ=255)

                                                   cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:3182582/71‑1 (MQ=255)

                                                   cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:1773326/71‑1 (MQ=255)

                                                   cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:507759/71‑1 (MQ=255)

                                                    acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa              >  1:620774/4‑70 (MQ=255)

                                                    acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa              >  1:2643147/4‑70 (MQ=255)

                                                    acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:2348795/70‑1 (MQ=255)

                                                    acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              >  1:3648120/1‑70 (MQ=255)

                                                     caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            >  1:2175401/1‑55 (MQ=255)

                                                     caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa            <  1:1876149/71‑1 (MQ=255)

                                                     caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa            >  1:369498/1‑71 (MQ=255)

                                                      gaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGTTGGTGTTaaaa            <  1:3035046/69‑1 (MQ=255)

                                                       aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:1053184/1‑54 (MQ=255)

                                                       aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:3254630/1‑54 (MQ=255)

                                                       aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt          <  1:2386780/71‑1 (MQ=255)

                                                        aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc         >  1:3152333/1‑71 (MQ=255)

                                                        aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc         <  1:2069571/71‑1 (MQ=255)

                                                        aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg         >  1:643712/1‑70 (MQ=255)

                                                          gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             <  1:837689/49‑1 (MQ=255)

                                                          gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGa       <  1:1154618/71‑1 (MQ=255)

                                                            aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:1236744/1‑49 (MQ=255)

                                                             aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                         <  1:1991982/50‑1 (MQ=255)

                                                             aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:467502/61‑1 (MQ=255)

                                                             aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    <  1:1703473/71‑1 (MQ=255)

                                                             aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    >  1:1096749/1‑71 (MQ=255)

                                                             aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    >  1:2725876/1‑71 (MQ=255)

                                                             aTGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    >  1:787359/1‑71 (MQ=255)

                                                              tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt          >  1:2888344/1‑64 (MQ=255)

                                                              tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt          >  1:696586/1‑64 (MQ=255)

                                                              tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    >  1:2521322/1‑70 (MQ=255)

                                                               ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt                 >  1:3820079/1‑56 (MQ=255)

                                                               ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa  >  1:3016953/1‑71 (MQ=255)

                                                                gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                >  1:2238315/1‑40 (MQ=255)

                                                                gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                >  1:1531108/1‑40 (MQ=255)

                                                                gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt          >  1:3011779/1‑62 (MQ=255)

                                                                     gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGaa      >  1:2631467/1‑61 (MQ=255)

ATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGA  >  minE/2130560‑2130693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A