Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,331 G→A 25.0% L423L (CTA→TTA) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3310GA25.0% 134.5 / 43.8 76L423L (CTA→TTA) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (22/35);  new base A (6/13);  total (28/48)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCC  >  minE/2595262‑2595401
                                                                     |                                                                      
aGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgc                                                                       <  1:3577484/71‑1 (MQ=255)
  gCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgcgc                                                                     <  1:828145/71‑1 (MQ=255)
      ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                                 >  1:3680107/1‑71 (MQ=255)
      ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAg                                                                  >  1:2377166/1‑70 (MQ=255)
       gTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcg                                                                <  1:3544600/71‑1 (MQ=255)
       gTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcg                                                                <  1:523028/71‑1 (MQ=255)
         tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcg                                                                >  1:1493478/1‑69 (MQ=255)
          gtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag                                                             >  1:2170121/1‑71 (MQ=255)
          gtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcag                                                             >  1:2165543/1‑71 (MQ=255)
           tgATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggc                                                            <  1:3812469/71‑1 (MQ=255)
           tgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAg                                                                  >  1:88660/1‑65 (MQ=255)
             aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagcc                                                           <  1:2661785/70‑1 (MQ=255)
                gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccagag                                                       <  1:3065263/70‑3 (MQ=255)
                gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccagag                                                       <  1:3069942/70‑3 (MQ=255)
                gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                        <  1:570129/69‑2 (MQ=255)
                aaTGGTAATGTTGCTCATAACAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCg                                                       <  1:2762614/71‑1 (MQ=255)
                 aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                            >  1:3927779/1‑65 (MQ=255)
                 aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                      >  1:1962623/1‑71 (MQ=255)
                 aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGt                                                      <  1:570583/71‑1 (MQ=255)
                     tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca                                                  <  1:820837/71‑1 (MQ=255)
                       aTGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaa                                                 <  1:3403041/70‑1 (MQ=255)
                        tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                                >  1:3213169/1‑70 (MQ=255)
                        tGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGt                                                      <  1:3184317/64‑1 (MQ=255)
                        tGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAAc                                               <  1:3837190/71‑1 (MQ=255)
                         gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                  <  1:88415/67‑1 (MQ=255)
                         gTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                        <  1:3459494/61‑2 (MQ=255)
                         gTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                        <  1:3763272/61‑2 (MQ=255)
                         gTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaa                                                 >  1:1310177/1‑68 (MQ=255)
                           tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAg                                             >  1:2276807/1‑70 (MQ=255)
                            gCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGc                                            <  1:3681216/70‑1 (MQ=255)
                             cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                           >  1:3836341/1‑70 (MQ=255)
                              tCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGc                                            <  1:3183512/68‑1 (MQ=255)
                              tCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcacc                                         >  1:81486/1‑70 (MQ=255)
                               cATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                                >  1:559926/1‑63 (MQ=255)
                                atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                       >  1:429480/1‑71 (MQ=255)
                                atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcag                                        <  1:3586054/70‑1 (MQ=255)
                                atatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaaa                                                <  1:3632121/62‑1 (MQ=255)
                                   tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                     <  1:383569/70‑1 (MQ=255)
                                   tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                     <  1:2755530/70‑1 (MQ=255)
                                     aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                  >  1:10222/1‑71 (MQ=255)
                                       tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGt                                 >  1:1847403/1‑70 (MQ=255)
                                         tctcTTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa                               <  1:2217803/70‑1 (MQ=255)
                                            cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                            >  1:2259166/1‑70 (MQ=255)
                                             aatACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                          <  1:3616152/69‑1 (MQ=255)
                                             aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                           <  1:2522409/70‑2 (MQ=255)
                                             aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                              <  1:737032/67‑1 (MQ=255)
                                             aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                              <  1:3633394/67‑1 (MQ=255)
                                              ttACAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                         <  1:3458706/71‑1 (MQ=255)
                                              ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                        <  1:2848514/40‑1 (MQ=255)
                                              ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                        <  1:955717/40‑1 (MQ=255)
                                              ttACAAACTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                         <  1:3817304/71‑1 (MQ=255)
                                               tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                        >  1:2860903/1‑71 (MQ=255)
                                                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                      <  1:2732030/71‑1 (MQ=255)
                                                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                      >  1:3770257/1‑71 (MQ=255)
                                                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                       <  1:1120769/70‑1 (MQ=255)
                                                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                       <  1:2117169/70‑1 (MQ=255)
                                                  aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                     <  1:2263014/39‑1 (MQ=255)
                                                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   >  1:3918337/1‑71 (MQ=255)
                                                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:3201099/71‑1 (MQ=255)
                                                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:743724/71‑1 (MQ=255)
                                                     gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg                   >  1:2285033/1‑68 (MQ=255)
                                                        aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc                <  1:483706/70‑1 (MQ=255)
                                                         aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg              <  1:3658085/71‑1 (MQ=255)
                                                         aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg               >  1:959590/1‑70 (MQ=255)
                                                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:3873682/65‑1 (MQ=255)
                                                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg              >  1:3161442/1‑70 (MQ=255)
                                                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa             <  1:2954813/71‑1 (MQ=255)
                                                           gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg              >  1:2398976/1‑69 (MQ=255)
                                                             gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt          <  1:743734/71‑1 (MQ=255)
                                                              agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa         <  1:1478869/71‑1 (MQ=255)
                                                              agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa         <  1:3810850/71‑1 (MQ=255)
                                                                aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat       <  1:274209/71‑1 (MQ=255)
                                                                 cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat       <  1:3560902/70‑1 (MQ=255)
                                                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat     <  1:43544/71‑1 (MQ=255)
                                                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat     >  1:2573543/1‑71 (MQ=255)
                                                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat     >  1:1452255/1‑71 (MQ=255)
                                                                   tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                    <  1:2627965/55‑1 (MQ=255)
                                                                   tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc    >  1:3292052/1‑71 (MQ=255)
                                                                     gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc  >  1:1454587/1‑71 (MQ=255)
                                                                     |                                                                      
AGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCC  >  minE/2595262‑2595401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A