Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,595,368 |
G→A |
18.6% |
T410T (ACC→ACT) |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,595,368 | 0 | G | A | 18.6%
| 166.7
/ 29.6
| 75 | T410T (ACC→ACT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (26/35); new base A (12/2); total (38/37) |
| Fisher's exact test for biased strand distribution p-value = 6.17e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
AGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTG > minE/2595299‑2595436
|
aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg > 1:10222/1‑71 (MQ=255)
tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGt > 1:1847403/1‑70 (MQ=255)
tctcTTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa < 1:2217803/70‑1 (MQ=255)
cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:2259166/1‑70 (MQ=255)
aatACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa < 1:3616152/69‑1 (MQ=255)
aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac < 1:2522409/70‑2 (MQ=255)
aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt < 1:3633394/67‑1 (MQ=255)
aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt < 1:737032/67‑1 (MQ=255)
ttACAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa < 1:3458706/71‑1 (MQ=255)
ttACAAACTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa < 1:3817304/71‑1 (MQ=255)
tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:2860903/1‑71 (MQ=255)
cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:2732030/71‑1 (MQ=255)
cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc > 1:3770257/1‑71 (MQ=255)
cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc < 1:2117169/70‑1 (MQ=255)
cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc < 1:1120769/70‑1 (MQ=255)
aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3201099/71‑1 (MQ=255)
aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:743724/71‑1 (MQ=255)
aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:3918337/1‑71 (MQ=255)
gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg > 1:2285033/1‑68 (MQ=255)
aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc < 1:483706/70‑1 (MQ=255)
aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:3658085/71‑1 (MQ=255)
aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:959590/1‑70 (MQ=255)
agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3873682/65‑1 (MQ=255)
agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:3161442/1‑70 (MQ=255)
agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa < 1:2954813/71‑1 (MQ=255)
gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:2398976/1‑69 (MQ=255)
gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:743734/71‑1 (MQ=255)
agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:3810850/71‑1 (MQ=255)
agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:1478869/71‑1 (MQ=255)
aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:274209/71‑1 (MQ=255)
cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:3560902/70‑1 (MQ=255)
gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat < 1:43544/71‑1 (MQ=255)
gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:1452255/1‑71 (MQ=255)
gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:2573543/1‑71 (MQ=255)
tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt < 1:2627965/55‑1 (MQ=255)
tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc > 1:3292052/1‑71 (MQ=255)
gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1454587/1‑71 (MQ=255)
gcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1856021/1‑69 (MQ=255)
ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:1844456/48‑1 (MQ=255)
cAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaag > 1:3491374/1‑69 (MQ=255)
cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa > 1:1749108/1‑61 (MQ=255)
cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc > 1:206664/1‑71 (MQ=255)
cgcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa > 1:114326/2‑59 (MQ=255)
aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:609362/71‑1 (MQ=255)
gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:2844051/1‑67 (MQ=255)
gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:3247024/1‑67 (MQ=255)
cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:681841/1‑71 (MQ=255)
cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt > 1:2394515/3‑70 (MQ=255)
cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1171195/71‑1 (MQ=255)
ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:1393225/2‑69 (MQ=255)
agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg > 1:1978763/1‑71 (MQ=255)
gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:163676/1‑71 (MQ=255)
gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt < 1:2919751/71‑1 (MQ=255)
ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:3816696/66‑1 (MQ=255)
cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa < 1:3788270/48‑1 (MQ=255)
cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:1325139/1‑71 (MQ=255)
agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata > 1:3429149/1‑53 (MQ=255)
cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:3780660/1‑52 (MQ=255)
acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt > 1:1836209/1‑71 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:2052139/1‑41 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:774322/1‑41 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:2133865/1‑51 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc < 1:2022787/50‑1 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc < 1:636647/50‑1 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1452662/60‑1 (MQ=255)
acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:468464/1‑66 (MQ=255)
caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc > 1:110598/1‑71 (MQ=255)
aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:470834/1‑71 (MQ=255)
aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:3412815/69‑1 (MQ=255)
aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:2816182/69‑1 (MQ=255)
aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:3946538/1‑68 (MQ=255)
aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:244248/1‑68 (MQ=255)
aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:2123610/37‑1 (MQ=255)
aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCg > 1:900169/1‑70 (MQ=255)
acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:1270859/71‑1 (MQ=255)
acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:551815/71‑1 (MQ=255)
caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:1122091/1‑70 (MQ=255)
caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:2450722/1‑70 (MQ=255)
caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:982072/71‑1 (MQ=255)
caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:1360995/71‑1 (MQ=255)
ggcaggGTCATGATAATCCCTGTACGGAAGTAATATc > 1:3410296/1‑37 (MQ=255)
ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:505196/1‑71 (MQ=255)
ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:3790670/1‑71 (MQ=255)
caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt > 1:2703927/1‑71 (MQ=255)
aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg < 1:3383163/46‑1 (MQ=255)
aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg > 1:1778612/1‑71 (MQ=255)
|
AGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTG > minE/2595299‑2595436
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
