Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,521 T→C 23.2% E359E (GAA→GAG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5210TC23.2% 235.9 / 73.5 129E359E (GAA→GAG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (52/47);  new base C (19/11);  total (71/58)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTG  >  minE/2595455‑2595590
                                                                  |                                                                     
    cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa                                                                >  1:3749195/1‑70 (MQ=255)
     tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt                                                               >  1:1270227/1‑70 (MQ=255)
       ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa                                                             <  1:496167/70‑1 (MQ=255)
       ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa                                                             <  1:3592426/70‑1 (MQ=255)
       ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc                                                            >  1:2744289/1‑71 (MQ=255)
       ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc                                                            <  1:686166/71‑1 (MQ=255)
        cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACg                                                           >  1:3597233/1‑71 (MQ=255)
         aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCGATCACATTGGCATAAATCATTGCCTCTTTGACGACGc                                                          >  1:1067913/1‑71 (MQ=255)
         aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc                                                          <  1:2056505/71‑1 (MQ=255)
          aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGcc                                                         <  1:448498/71‑1 (MQ=255)
          aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc                                                          >  1:1826369/1‑70 (MQ=255)
           ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCa                                                        <  1:966097/71‑1 (MQ=255)
           tcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc                                                          >  1:3146126/3‑69 (MQ=255)
              gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc                                                     <  1:1039489/71‑3 (MQ=255)
                gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc                                                   >  1:3629754/1‑71 (MQ=255)
                 tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                  <  1:1716718/71‑1 (MQ=255)
                   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                 <  1:3632583/70‑1 (MQ=255)
                   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                >  1:3045419/1‑71 (MQ=255)
                   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                >  1:2282745/1‑71 (MQ=255)
                   aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt                                                                  >  1:1575010/1‑53 (MQ=255)
                   aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt                                                                  >  1:3659092/1‑53 (MQ=255)
                   aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg                                                       <  1:373726/64‑1 (MQ=255)
                   aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg                                                       <  1:2776004/64‑1 (MQ=255)
                    ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGctt                                                                      <  1:1153299/48‑1 (MQ=255)
                    ttttCGGGCCTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                               <  1:281000/71‑2 (MQ=255)
                      ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                             >  1:1834918/1‑71 (MQ=255)
                       tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                            >  1:1079007/1‑71 (MQ=255)
                       tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                            >  1:1335928/1‑70 (MQ=255)
                        cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                            <  1:1014757/70‑2 (MQ=255)
                         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                           <  1:2603902/70‑1 (MQ=255)
                         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                           >  1:1098596/1‑70 (MQ=255)
                         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc                                          <  1:2138816/71‑1 (MQ=255)
                          gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                         <  1:3081292/71‑1 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg                                                                 <  1:803192/46‑1 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg                                                                 <  1:2643761/46‑1 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg                                                                 <  1:2384325/46‑1 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa                                                                >  1:884215/1‑47 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                               <  1:1221579/64‑1 (MQ=255)
                           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                               <  1:3699881/64‑1 (MQ=255)
                            cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                            >  1:3733789/1‑66 (MQ=255)
                            cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt                                        >  1:556/1‑70 (MQ=255)
                              cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt                                                     >  1:1126735/1‑55 (MQ=255)
                               aaaTCGCAGCCAATCACATTGGCATAAACCATCGctt                                                                      >  1:3549646/1‑37 (MQ=255)
                               aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                    >  1:2797251/1‑71 (MQ=255)
                               aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                    >  1:3493069/1‑71 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                              >  1:96736/1‑60 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                              >  1:3524820/1‑60 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   >  1:899769/1‑71 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   >  1:1132875/1‑71 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   <  1:2865012/71‑1 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   >  1:1919377/1‑71 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   >  1:2405114/1‑71 (MQ=255)
                                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   <  1:2090554/71‑1 (MQ=255)
                                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                               >  1:743546/1‑58 (MQ=255)
                                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                   >  1:1814704/1‑70 (MQ=255)
                                 aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                         >  1:3412466/1‑64 (MQ=255)
                                 aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                     <  1:3923911/68‑1 (MQ=255)
                                   cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                 >  1:810901/1‑70 (MQ=255)
                                     cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                              <  1:744751/71‑1 (MQ=255)
                                     cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                              <  1:2174292/71‑1 (MQ=255)
                                      aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc                             >  1:2383520/1‑69 (MQ=255)
                                      aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc                             <  1:1588070/71‑1 (MQ=255)
                                       gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                            <  1:925202/71‑1 (MQ=255)
                                        ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCa                                      <  1:1114362/60‑1 (MQ=255)
                                         gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg                          <  1:1149530/70‑1 (MQ=255)
                                         cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                          >  1:3797257/1‑70 (MQ=255)
                                         cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                           <  1:1894529/70‑1 (MQ=255)
                                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                       <  1:3800795/57‑1 (MQ=255)
                                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                         <  1:301922/71‑1 (MQ=255)
                                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                         >  1:3837660/1‑71 (MQ=255)
                                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                  <  1:794708/45‑1 (MQ=255)
                                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                 >  1:3140959/1‑46 (MQ=255)
                                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                         <  1:1290411/54‑1 (MQ=255)
                                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                         >  1:2994059/1‑70 (MQ=255)
                                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc                        >  1:3386963/1‑70 (MQ=255)
                                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                   >  1:2580474/1‑59 (MQ=255)
                                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                        >  1:2944604/1‑70 (MQ=255)
                                              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc                     <  1:3891851/71‑1 (MQ=255)
                                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                    <  1:573784/71‑1 (MQ=255)
                                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                    <  1:1891077/71‑1 (MQ=255)
                                                aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                   <  1:2081315/71‑1 (MQ=255)
                                                 ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                  <  1:1018982/71‑1 (MQ=255)
                                                 ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                  <  1:3657448/71‑1 (MQ=255)
                                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                  >  1:331902/1‑70 (MQ=255)
                                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                  >  1:283908/1‑70 (MQ=255)
                                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                  >  1:2753474/1‑70 (MQ=255)
                                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                     <  1:3334613/47‑1 (MQ=255)
                                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                           <  1:778934/57‑1 (MQ=255)
                                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                           >  1:2829819/1‑56 (MQ=255)
                                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg             <  1:1832053/70‑1 (MQ=255)
                                                        aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc           >  1:3243634/1‑71 (MQ=255)
                                                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                              >  1:1411956/1‑35 (MQ=255)
                                                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc           <  1:62314/70‑1 (MQ=255)
                                                             aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                     >  1:1860656/1‑56 (MQ=255)
                                                               cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                           <  1:1589313/48‑1 (MQ=255)
                                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat    <  1:1738194/70‑1 (MQ=255)
                                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat    <  1:1697944/70‑1 (MQ=255)
                                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt   >  1:1389065/1‑71 (MQ=255)
                                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                          <  1:349717/47‑1 (MQ=255)
                                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg  <  1:2641752/71‑1 (MQ=255)
                                                                  |                                                                     
CAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTG  >  minE/2595455‑2595590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A