Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,548 G→C 16.5% G350G (GGC→GGG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5480GC16.5% 225.3 / 34.4 97G350G (GGC→GGG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (37/44);  new base C (9/7);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 101 total aligned reads displayed.

TCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGG  >  minE/2595478‑2595612
                                                                      |                                                                
tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                                  >  1:1079007/1‑71 (MQ=255)
  ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                                 <  1:2603902/70‑1 (MQ=255)
  ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                                 >  1:1098596/1‑70 (MQ=255)
  ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc                                                                <  1:2138816/71‑1 (MQ=255)
   gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                               <  1:3081292/71‑1 (MQ=255)
   gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                               <  1:3379826/71‑1 (MQ=255)
     cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                                  >  1:3733789/1‑66 (MQ=255)
     cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt                                                              >  1:556/1‑70 (MQ=255)
        aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                          >  1:2797251/1‑71 (MQ=255)
        aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                          >  1:3493069/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:1673561/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:1919377/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         <  1:2865012/71‑1 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         <  1:2090554/71‑1 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:2105044/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:899769/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:1132875/1‑71 (MQ=255)
         aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:2405114/1‑71 (MQ=255)
         aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                         >  1:1814704/1‑70 (MQ=255)
         aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                         >  1:453421/1‑70 (MQ=255)
          aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                               >  1:3412466/1‑64 (MQ=255)
          aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                                           <  1:3923911/68‑1 (MQ=255)
            cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                       >  1:810901/1‑70 (MQ=255)
              cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                                    <  1:744751/71‑1 (MQ=255)
              cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                                    <  1:2174292/71‑1 (MQ=255)
               aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc                                                   >  1:2383520/1‑69 (MQ=255)
               aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc                                                   <  1:1588070/71‑1 (MQ=255)
                gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                                                  <  1:925202/71‑1 (MQ=255)
                 ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCa                                                            <  1:1114362/60‑1 (MQ=255)
                 ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg                                                  >  1:2386721/1‑70 (MQ=255)
                  gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg                                                <  1:1149530/70‑1 (MQ=255)
                  cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                                >  1:3797257/1‑70 (MQ=255)
                  cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                 <  1:1894529/70‑1 (MQ=255)
                   aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac                                               >  1:782482/1‑69 (MQ=255)
                   aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                                <  1:2834251/70‑2 (MQ=255)
                   aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                             <  1:3800795/57‑1 (MQ=255)
                   aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                               <  1:301922/71‑1 (MQ=255)
                   aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                               >  1:3837660/1‑71 (MQ=255)
                    aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                               <  1:1290411/54‑1 (MQ=255)
                    aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                               >  1:2994059/1‑70 (MQ=255)
                     taacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:377381/3‑59 (MQ=255)
                     tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                              >  1:3386963/1‑70 (MQ=255)
                     tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                         >  1:2580474/1‑59 (MQ=255)
                     tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                              >  1:2944604/1‑70 (MQ=255)
                     tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                              >  1:729627/1‑70 (MQ=255)
                       acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat                                           >  1:831528/1‑70 (MQ=255)
                       acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc                                           <  1:3891851/71‑1 (MQ=255)
                        caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                          <  1:1891077/71‑1 (MQ=255)
                        caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                          <  1:3866880/71‑1 (MQ=255)
                        caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                          <  1:573784/71‑1 (MQ=255)
                         aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                         <  1:2081315/71‑1 (MQ=255)
                          ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                        <  1:1018982/71‑1 (MQ=255)
                          ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                        <  1:3657448/71‑1 (MQ=255)
                           tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                        >  1:331902/1‑70 (MQ=255)
                           tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                        >  1:283908/1‑70 (MQ=255)
                           tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                        >  1:2753474/1‑70 (MQ=255)
                               aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                           <  1:3172012/47‑1 (MQ=255)
                               aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                           <  1:3334613/47‑1 (MQ=255)
                               aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                 <  1:778934/57‑1 (MQ=255)
                                tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                 >  1:2829819/1‑56 (MQ=255)
                                tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg                                   <  1:1832053/70‑1 (MQ=255)
                                tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                  >  1:3470445/1‑71 (MQ=255)
                                 aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                 >  1:3243634/1‑71 (MQ=255)
                                  aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                 <  1:62314/70‑1 (MQ=255)
                                      aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                           >  1:1860656/1‑56 (MQ=255)
                                       tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                           >  1:1873291/1‑71 (MQ=255)
                                        cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                                 <  1:1589313/48‑1 (MQ=255)
                                         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                         >  1:1827212/1‑55 (MQ=255)
                                         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                          <  1:1697944/70‑1 (MQ=255)
                                         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                          <  1:1738194/70‑1 (MQ=255)
                                         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                         <  1:1414431/71‑1 (MQ=255)
                                         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                         >  1:1389065/1‑71 (MQ=255)
                                          cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                <  1:349717/47‑1 (MQ=255)
                                          cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                        <  1:2641752/71‑1 (MQ=255)
                                          cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                        >  1:1063083/1‑71 (MQ=255)
                                           ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                     <  1:280419/57‑1 (MQ=255)
                                             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                      <  1:3518380/70‑1 (MQ=255)
                                             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                       <  1:1413438/69‑1 (MQ=255)
                                             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                       <  1:2901691/69‑1 (MQ=255)
                                             cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                      >  1:3001570/1‑70 (MQ=255)
                                              tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCa                    >  1:1299074/1‑71 (MQ=255)
                                                tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                    <  1:1517361/69‑1 (MQ=255)
                                                 gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                  <  1:2228124/70‑1 (MQ=255)
                                                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat                <  1:501691/71‑1 (MQ=255)
                                                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                   >  1:1404703/1‑68 (MQ=255)
                                                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                   >  1:3220389/1‑68 (MQ=255)
                                                   tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata               <  1:3771082/71‑1 (MQ=255)
                                                   tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                 >  1:3037491/1‑69 (MQ=255)
                                                    aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                    >  1:1638895/1‑49 (MQ=255)
                                                     aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag              <  1:888457/70‑1 (MQ=255)
                                                        ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                         >  1:2593501/1‑40 (MQ=255)
                                                         cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa            >  1:1563804/1‑68 (MQ=255)
                                                          aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga          >  1:1388102/1‑69 (MQ=255)
                                                          aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga        <  1:3061942/71‑1 (MQ=255)
                                                           gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc                     >  1:2859084/1‑57 (MQ=255)
                                                            tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATAtt                         <  1:2355834/50‑1 (MQ=255)
                                                             tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa     <  1:50266/71‑1 (MQ=255)
                                                               ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa    <  1:3224139/70‑1 (MQ=255)
                                                                cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg  <  1:2904511/71‑3 (MQ=255)
                                                                cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg  <  1:201724/71‑3 (MQ=255)
                                                                      |                                                                
TCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGG  >  minE/2595478‑2595612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A