Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,596,157 |
G→T |
28.8% |
A147A (GCC→GCA) |
arsB ← |
arsenite/antimonite transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,596,157 | 0 | G | T | 28.8%
| 100.7
/ 28.2
| 52 | A147A (GCC→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (16/21); new base T (6/9); total (22/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 |
CGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCAT > minE/2596087‑2596225
|
cGAGGCCAAAGAAATCAGCGTAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCg < 1:3030136/71‑1 (MQ=255)
aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:1731717/71‑1 (MQ=255)
gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCaa > 1:2084086/1‑71 (MQ=255)
ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:2061612/71‑1 (MQ=255)
gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:1599426/70‑3 (MQ=255)
aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATaaa < 1:2122001/71‑1 (MQ=255)
aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa > 1:333578/1‑71 (MQ=255)
aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa > 1:3449102/1‑71 (MQ=255)
aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:3510425/1‑71 (MQ=255)
cGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgc > 1:70191/1‑70 (MQ=255)
ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGTTATCGGCAATGAATCCGGCCGCCa > 1:3568100/1‑71 (MQ=255)
gaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAtga < 1:2985791/70‑3 (MQ=255)
aCGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgcc > 1:56520/1‑63 (MQ=255)
aCGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:1297938/71‑1 (MQ=255)
aCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa > 1:2573727/1‑53 (MQ=255)
gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt < 1:2702354/51‑1 (MQ=255)
gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:2419478/71‑3 (MQ=255)
gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca < 1:1542783/70‑2 (MQ=255)
tatTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAAc > 1:2097649/1‑71 (MQ=255)
ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:487693/65‑1 (MQ=255)
tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGaa < 1:3465117/67‑1 (MQ=255)
tCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:3485191/1‑70 (MQ=255)
aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCg < 1:3889235/51‑1 (MQ=255)
aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa < 1:819963/70‑1 (MQ=255)
ccAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATcc > 1:991115/1‑49 (MQ=255)
ccAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgt < 1:307056/71‑2 (MQ=255)
cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgcc < 1:416266/55‑1 (MQ=255)
cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3783559/71‑1 (MQ=255)
aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTgca > 1:3436126/1‑50 (MQ=255)
aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt > 1:2497266/1‑71 (MQ=255)
ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc > 1:3315800/1‑70 (MQ=255)
gTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:1555969/39‑1 (MQ=255)
gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc < 1:955960/36‑1 (MQ=12)
ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAg > 1:1119177/1‑68 (MQ=255)
ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2402450/70‑1 (MQ=255)
gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGc < 1:2267886/70‑1 (MQ=255)
agaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg < 1:58925/42‑1 (MQ=255)
agaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCt < 1:1856390/71‑1 (MQ=255)
aaaCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACATAGGCCAGCGTCGTGccc > 1:548452/3‑70 (MQ=255)
gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:3057455/63‑1 (MQ=255)
aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg < 1:2091714/71‑4 (MQ=255)
aaTAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc < 1:2481318/66‑1 (MQ=255)
aTAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:868664/71‑1 (MQ=255)
gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc < 1:3213076/71‑1 (MQ=255)
gCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:1278145/1‑64 (MQ=255)
gCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAccc > 1:2921311/1‑70 (MQ=255)
cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAcc > 1:1064160/1‑68 (MQ=255)
cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:857920/71‑1 (MQ=255)
cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:988578/71‑1 (MQ=255)
aGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa > 1:2518166/1‑70 (MQ=255)
ggCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa > 1:2130549/1‑69 (MQ=255)
cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:3818098/71‑1 (MQ=255)
cGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcag > 1:2087774/1‑70 (MQ=255)
ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCAt < 1:2851223/69‑1 (MQ=255)
|
CGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCAT > minE/2596087‑2596225
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
