Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,234,526 |
T→C |
19.1% |
T28A (ACG→GCG) |
dsbB ← |
oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,234,526 | 0 | T | C | 19.1%
| 100.2
/ 20.4
| 47 | T28A (ACG→GCG) | dsbB | oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base T (21/17); new base C (6/3); total (27/20) |
| Fisher's exact test for biased strand distribution p-value = 7.13e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 |
ACGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAT > W3110S.gb/1234457‑1234587
|
aCGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:3245820/71‑1 (MQ=255)
cgcAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:270278/68‑1 (MQ=255)
cgcAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTc < 1:264196/68‑1 (MQ=255)
cAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTc < 1:3593372/71‑1 (MQ=255)
gTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGt > 1:858988/1‑71 (MQ=255)
aTAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCa > 1:1380600/1‑59 (MQ=255)
aaaTACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGt < 1:929932/65‑1 (MQ=255)
cAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGt < 1:3229437/71‑1 (MQ=255)
cAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGt < 1:1566813/71‑1 (MQ=255)
agagCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTa < 1:3088359/71‑1 (MQ=255)
cACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACg > 1:1091464/1‑71 (MQ=255)
cGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGc > 1:4677647/1‑70 (MQ=255)
aaGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:4738394/71‑1 (MQ=255)
aaGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:4737814/71‑1 (MQ=255)
aGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATc > 1:4818023/1‑70 (MQ=255)
ggTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:1617288/1‑71 (MQ=255)
ggTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:3827136/1‑71 (MQ=255)
gTTTCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTaa > 1:1430598/1‑60 (MQ=255)
tttCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACa < 1:3996088/71‑1 (MQ=255)
ttCAGTAACATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTa < 1:100281/57‑1 (MQ=255)
aaCATCACATGCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTaaa > 1:30600/1‑53 (MQ=255)
tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGccc > 1:3186209/1‑71 (MQ=255)
tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGccc > 1:4561736/1‑71 (MQ=255)
tCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCAT‑AACAGCCACGCGcccc > 1:4648613/1‑71 (MQ=255)
acaTGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCa > 1:1169389/1‑62 (MQ=255)
acaTGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCg > 1:4306991/1‑71 (MQ=255)
tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTa < 1:752101/42‑1 (MQ=255)
tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc < 1:3198423/71‑1 (MQ=255)
tGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGcc < 1:4896957/71‑1 (MQ=255)
gCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:5033711/1‑71 (MQ=255)
gCTGGAACCACAGCGCCGCCTGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:2758001/1‑71 (MQ=255)
gCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:1187675/1‑71 (MQ=255)
gCTGGAACCACAGCGCCGCCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt > 1:5005557/1‑71 (MQ=255)
cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTaaa > 1:1087280/1‑42 (MQ=255)
cTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTaaa > 1:4605975/1‑42 (MQ=255)
tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACTCCATCAACAGCCACGCGCCCCGGCCt < 1:2508341/69‑1 (MQ=255)
tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt < 1:3736456/69‑1 (MQ=255)
tGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCt < 1:3636711/69‑1 (MQ=255)
ggAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATc < 1:2946194/47‑1 (MQ=255)
gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtgtg < 1:686516/71‑1 (MQ=255)
gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtg > 1:2343013/1‑69 (MQ=255)
gAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTtg > 1:2020500/1‑69 (MQ=255)
aaCCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCaa > 1:606930/1‑47 (MQ=255)
acaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGaa > 1:753903/1‑68 (MQ=255)
acaGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAt > 1:2042176/1‑71 (MQ=255)
caGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGcc > 1:122744/1‑54 (MQ=255)
caGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAt > 1:3536106/1‑70 (MQ=255)
tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:4354522/42‑1 (MQ=255)
tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1912415/42‑1 (MQ=255)
tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1889001/42‑1 (MQ=255)
tCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCAcg < 1:1878546/42‑1 (MQ=255)
|
ACGCGCAGCGTTCATAAATACAGAGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACAT > W3110S.gb/1234457‑1234587
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
