Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,082,018 |
A→G |
38.3% |
I237I (ATT→ATC) |
yeeA ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,082,018 | 0 | A | G | 38.3%
| 40.8
/ 67.9
| 60 | I237I (ATT→ATC) | yeeA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (18/19); new base G (11/12); total (29/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01 |
ATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTC > W3110S.gb/2081952‑2082085
|
aTGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATg > 1:587093/1‑70 (MQ=255)
cTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTg < 1:3446883/71‑1 (MQ=255)
cgcgTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATAcc > 1:2908305/1‑71 (MQ=255)
cccAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCatat < 1:1329931/71‑1 (MQ=255)
cccAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCatat < 1:4300285/71‑1 (MQ=255)
aTGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCt > 1:3982377/1‑51 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTgg > 1:3930016/1‑52 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGa > 1:1382898/1‑53 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGAt > 1:4364380/1‑70 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATg > 1:2320875/1‑46 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGAtt > 1:639142/1‑71 (MQ=255)
tGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGAtt > 1:1984616/1‑71 (MQ=255)
gCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATc < 1:1660350/66‑1 (MQ=255)
gCATTGATATGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATc < 1:1910241/66‑1 (MQ=255)
cATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGAt > 1:1042584/1‑43 (MQ=255)
cATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGAtttt > 1:3286941/1‑71 (MQ=255)
aTTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTaa > 1:4189918/1‑41 (MQ=255)
aTTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTaa > 1:4142710/1‑41 (MQ=255)
aTTGATTTGCAACTCCACCATACAAACAAGATTGCGGTTa > 1:490076/1‑40 (MQ=38)
ttGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGAttt < 1:4570879/68‑1 (MQ=255)
ttGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGAttt < 1:3287124/68‑1 (MQ=255)
ttGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTgg < 1:4473241/71‑1 (MQ=255)
ttGATTTGCAACTCCAGCATACAAACCAGAATGCGGTTGATGGTCTGGATACCTTCATATATCGAttt < 1:3881698/68‑1 (MQ=255)
gATTTGCAACTCCAGCATACAATCCAGATTGCGGTTa > 1:2336350/1‑37 (MQ=255)
gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTgg > 1:3180871/1‑46 (MQ=255)
gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGa > 1:3278307/1‑47 (MQ=255)
gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGa > 1:1280278/1‑47 (MQ=255)
gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCata > 1:1447414/1‑57 (MQ=255)
gATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa > 1:4299193/1‑71 (MQ=255)
aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa < 1:4173856/70‑1 (MQ=255)
ttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCata > 1:460608/1‑54 (MQ=255)
gCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa < 1:1097624/66‑1 (MQ=255)
aaCTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATa > 1:4342516/1‑66 (MQ=255)
aaCTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATa > 1:4856525/1‑66 (MQ=255)
aCTCCAGCATACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGaa > 1:1869751/1‑63 (MQ=255)
gCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTgg > 1:29767/1‑55 (MQ=255)
gCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTg < 1:4265965/71‑1 (MQ=255)
gCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTg < 1:806156/71‑1 (MQ=255)
aTACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCt < 1:880967/71‑1 (MQ=255)
aTACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCt < 1:2433270/71‑1 (MQ=255)
tACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACgg > 1:4949538/1‑59 (MQ=255)
tACAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCtt < 1:14609/66‑1 (MQ=255)
tACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGAttt < 1:1071624/49‑1 (MQ=255)
aCAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGAttt < 1:641537/48‑1 (MQ=255)
cAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATa > 1:2936819/1‑54 (MQ=255)
cAAACCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGc > 1:405794/1‑67 (MQ=255)
aaCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTggcg < 1:561006/70‑1 (MQ=255)
aCCAGATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTggcgg > 1:1469713/1‑70 (MQ=255)
aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa > 1:4500296/1‑49 (MQ=255)
aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa > 1:3628046/1‑49 (MQ=255)
ccAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGaa < 1:4446135/48‑1 (MQ=255)
gATTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGgcgc < 1:161822/70‑1 (MQ=255)
aTTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTg < 1:1042562/41‑1 (MQ=255)
aTTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTg < 1:2951188/41‑1 (MQ=255)
aTTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTg < 1:3916963/41‑1 (MQ=255)
aTTGCGGTTGATGGTCTGGATACCTTCATATATCGATTTTg < 1:4190907/41‑1 (MQ=255)
ttGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAAt < 1:718574/71‑1 (MQ=255)
cGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCa < 1:1926069/70‑1 (MQ=255)
cGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAg < 1:26516/71‑1 (MQ=255)
cGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAg < 1:4357959/71‑1 (MQ=255)
cGGTTAATGGTCTGGATACCTTCATATATCGATTTTGCaa > 1:4917117/1‑40 (MQ=255)
ggTTGATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGTTTTCtt < 1:3502806/51‑1 (MQ=255)
gTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTc > 1:4144023/1‑71 (MQ=255)
ttAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCttt < 1:1438199/50‑1 (MQ=255)
aaTGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCt > 1:279850/1‑51 (MQ=255)
|
ATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTC > W3110S.gb/2081952‑2082085
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
