Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,458,921 |
T→C |
42.6% |
K6K (AAA→AAG) |
nusG ← |
transcription termination factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,458,921 | 0 | T | C | 42.6%
| 30.0
/ 85.2
| 68 | K6K (AAA→AAG) | nusG | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base T (27/12); new base C (14/15); total (41/27) |
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAT > W3110S.gb/3458853‑3458989
|
tGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:4332719/71‑1 (MQ=255)
tGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt < 1:464384/70‑1 (MQ=255)
cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttttt > 1:3877885/1‑69 (MQ=255)
cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttttt > 1:1562635/1‑69 (MQ=255)
aCGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAg > 1:3137916/1‑68 (MQ=255)
aCGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAg > 1:3131710/1‑68 (MQ=255)
gCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:3393248/1‑66 (MQ=255)
gCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4373115/1‑66 (MQ=255)
cAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:2785390/71‑1 (MQ=255)
gACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGa > 1:835629/1‑71 (MQ=255)
aCGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGAc < 1:2920771/71‑1 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:3742858/1‑54 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:5073908/1‑54 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4147067/1‑55 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:2611097/1‑55 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:3919600/1‑70 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:3483874/1‑70 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:1539442/1‑70 (MQ=255)
gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACAtct > 1:4348803/1‑70 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGaa > 1:1840327/1‑71 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGaa < 1:1027038/71‑1 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGa < 1:3468602/70‑1 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAg < 1:4108184/69‑1 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGaa > 1:3326013/1‑71 (MQ=255)
cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGa < 1:3003086/70‑1 (MQ=255)
ccTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt > 1:3352055/1‑69 (MQ=255)
cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAg < 1:4348064/54‑1 (MQ=255)
ttCAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:4327800/42‑1 (MQ=255)
ttCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCa < 1:1950566/52‑1 (MQ=255)
tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGAc > 1:1791910/1‑54 (MQ=255)
tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4325674/1‑71 (MQ=255)
tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4746088/1‑71 (MQ=255)
tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:1183883/1‑71 (MQ=255)
tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:2728983/1‑71 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4917658/1‑42 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4711135/1‑70 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAgg > 1:634743/1‑43 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAg > 1:4186903/1‑45 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAg > 1:4635951/1‑45 (MQ=255)
cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGa > 1:3791125/1‑52 (MQ=255)
aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:2378759/1‑40 (MQ=255)
aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGcc < 1:3615988/70‑1 (MQ=255)
aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGcc < 1:3645875/70‑1 (MQ=255)
aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:3942340/1‑69 (MQ=255)
aCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTg < 1:2063311/71‑1 (MQ=255)
aCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAgg < 1:4271497/39‑1 (MQ=255)
ggAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa < 1:1915742/71‑1 (MQ=255)
aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt > 1:1592595/1‑67 (MQ=255)
aaaCGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg < 1:3340241/71‑1 (MQ=255)
aaaCGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:2668778/1‑71 (MQ=255)
aaCGCCAGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:4584/1‑70 (MQ=255)
ccTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg < 1:4832532/66‑1 (MQ=255)
tGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:2634571/70‑1 (MQ=255)
acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt > 1:3106490/1‑42 (MQ=255)
acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGaa > 1:3248535/1‑71 (MQ=255)
acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGaa > 1:1809168/1‑71 (MQ=255)
tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCg < 1:4700710/66‑1 (MQ=255)
tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:62103/71‑1 (MQ=255)
tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:1095103/71‑1 (MQ=255)
tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:4006862/71‑1 (MQ=255)
tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:104278/71‑1 (MQ=255)
ccAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATa > 1:179340/1‑57 (MQ=255)
ccAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGc > 1:3382160/1‑63 (MQ=255)
ccAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:5144942/70‑1 (MQ=255)
ccgcgcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:3371355/67‑1 (MQ=255)
cAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGa > 1:4246580/1‑54 (MQ=255)
cAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:5139877/69‑1 (MQ=255)
aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa > 1:4047698/1‑70 (MQ=255)
cgcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATa > 1:4842895/1‑70 (MQ=255)
gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:4622559/71‑1 (MQ=255)
gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:2901116/71‑1 (MQ=255)
gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:3608590/71‑1 (MQ=255)
gcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAc > 1:754848/1‑70 (MQ=255)
tttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAt < 1:103448/69‑1 (MQ=255)
|
TGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAT > W3110S.gb/3458853‑3458989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
