Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R2
|
316 |
65.6 |
5147759 |
97.4% |
5013917 |
60.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,443,410 |
G→A |
31.6% |
L179L (CTG→TTG) |
ytfJ ← |
predicted transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,443,410 | 0 | G | A | 31.6%
| 81.2
/ 45.0
| 57 | L179L (CTG→TTG) | ytfJ | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base A (14/4); total (30/27) |
| Fisher's exact test for biased strand distribution p-value = 1.19e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCA > W3110S.gb/4443341‑4443477
|
tAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag > 1:4582448/1‑70 (MQ=255)
tAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag > 1:2602940/1‑70 (MQ=255)
gATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGcaacag > 1:3863894/1‑71 (MQ=255)
gATAACGATAAGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGcagcag > 1:4253407/1‑71 (MQ=255)
gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCAt < 1:1189093/71‑1 (MQ=255)
gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCAt < 1:3418634/71‑1 (MQ=255)
gATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCAt > 1:3678353/1‑71 (MQ=255)
aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATc < 1:3440673/71‑1 (MQ=255)
aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCAt > 1:4208238/1‑70 (MQ=255)
aTAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATc < 1:5025293/71‑1 (MQ=255)
gAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtgc < 1:1344924/71‑1 (MQ=255)
aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtgctg < 1:3029105/71‑1 (MQ=255)
aaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg > 1:3366899/1‑70 (MQ=255)
ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:125499/63‑1 (MQ=255)
ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:4003263/1‑62 (MQ=255)
ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACt > 1:4657667/1‑63 (MQ=255)
ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCAc < 1:5125062/71‑1 (MQ=255)
ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGAAGCAGGTCCATCACt < 1:1745639/63‑1 (MQ=255)
attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:2031007/61‑1 (MQ=255)
attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACt < 1:4388598/61‑1 (MQ=255)
cAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag < 1:2122106/41‑1 (MQ=255)
aGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgt < 1:1700697/71‑1 (MQ=255)
aaTTTATGTTATTGCCATTATTTGAGCAACTTTTgcagc < 1:2892934/39‑1 (MQ=255)
aaTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt < 1:2845017/71‑1 (MQ=255)
aaTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtg > 1:5118150/1‑70 (MQ=255)
ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:1363280/1‑71 (MQ=255)
ttATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:4119916/1‑71 (MQ=255)
tATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAATtgc > 1:4416050/1‑50 (MQ=255)
ttATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAg > 1:2418824/1‑66 (MQ=255)
tGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAgcg > 1:126621/1‑64 (MQ=255)
ccATTATTTGAGCAACTTTTGCAACAGGTCCATCa > 1:4175008/1‑35 (MQ=255)
ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:1403497/1‑36 (MQ=255)
ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:1371383/1‑36 (MQ=255)
ccATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:3231219/1‑36 (MQ=255)
cATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCttt < 1:3293584/71‑1 (MQ=255)
attattTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg < 1:3573717/40‑1 (MQ=255)
attattTGAGCAACTTTTGCAGCAGGTCCATCACTtgctg < 1:620321/40‑1 (MQ=255)
ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACctct < 1:59518/46‑1 (MQ=255)
ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACctct < 1:408346/46‑1 (MQ=255)
tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCa > 1:2768341/1‑40 (MQ=255)
tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCa > 1:2605365/1‑40 (MQ=255)
attTGAGCAACTTTTGCAGCAGGTCCATCACTtgct > 1:796637/1‑36 (MQ=255)
attTGAGCAACTTTTGCAGCAGGTCCATCACTtgct > 1:2842641/1‑36 (MQ=255)
attTGAGCAAATTTTGCAGCAGGTCCATCACTtgctgc < 1:4447274/38‑1 (MQ=255)
tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:3063958/71‑1 (MQ=255)
tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:3895497/71‑1 (MQ=255)
tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGccc < 1:2809598/71‑1 (MQ=255)
tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGcc < 1:3269259/70‑1 (MQ=255)
ttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCAc > 1:4668892/1‑38 (MQ=255)
cAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCa < 1:687215/71‑1 (MQ=255)
aaCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCAc < 1:2637776/71‑1 (MQ=255)
aCTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCAcg > 1:4915825/1‑71 (MQ=255)
cTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgt < 1:723034/40‑1 (MQ=255)
tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt > 1:1952984/1‑40 (MQ=255)
tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt > 1:649190/1‑40 (MQ=255)
tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:4821292/1‑71 (MQ=255)
tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:5119964/1‑71 (MQ=255)
tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCtt < 1:5089025/54‑1 (MQ=255)
tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGa > 1:3063095/1‑71 (MQ=255)
tttGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCACGTCTTTGGCCCATTGCa > 1:1412369/1‑66 (MQ=255)
tGCAGCGGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:658750/1‑70 (MQ=255)
tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:1643923/1‑70 (MQ=255)
tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGAc > 1:1220122/1‑70 (MQ=255)
cagcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCa < 1:779065/70‑1 (MQ=255)
|
TAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCA > W3110S.gb/4443341‑4443477
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
