Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,014,659 |
C→A |
12.9% |
V470V (GTG→GTT) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,014,659 | 0 | C | A | 12.9%
| 169.4
/ 7.7
| 54 | V470V (GTG→GTT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (22/25); new base A (4/3); total (26/28) |
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 |
CGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACA > minE/1014590‑1014728
|
cgcCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTc > 1:266624/1‑70 (MQ=255)
cgcCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCa > 1:3186191/1‑71 (MQ=255)
tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:3255236/1‑68 (MQ=255)
cAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctgg < 1:398530/71‑1 (MQ=255)
cAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctgg < 1:495580/71‑1 (MQ=255)
gCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTgctggc < 1:1049621/67‑1 (MQ=255)
gCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCg < 1:3287757/71‑1 (MQ=255)
cGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCg < 1:2535967/69‑1 (MQ=255)
ggACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCg < 1:2467512/68‑1 (MQ=255)
ggACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGtt < 1:3375748/71‑1 (MQ=255)
ggACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGtt < 1:3619571/71‑1 (MQ=255)
gccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgc > 1:3591259/2‑55 (MQ=255)
gccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCt > 1:320690/2‑70 (MQ=255)
accGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCtt < 1:3607258/71‑1 (MQ=255)
ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCttt > 1:3542282/1‑71 (MQ=255)
ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCttt > 1:1037621/1‑71 (MQ=255)
ccGATACTGCCAATGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:705339/1‑52 (MQ=255)
ccGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:1518011/1‑52 (MQ=255)
cGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTc < 1:2295753/71‑1 (MQ=255)
aTACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCt < 1:1736244/66‑1 (MQ=255)
aTACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc < 1:1788725/71‑1 (MQ=255)
tACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGcc < 1:1873513/71‑1 (MQ=255)
cTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAg < 1:448507/71‑1 (MQ=255)
cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGcc < 1:1702727/65‑1 (MQ=255)
aaGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTg < 1:1357149/55‑1 (MQ=255)
aGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGAt > 1:1908248/1‑71 (MQ=255)
ggTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATc < 1:1931665/71‑1 (MQ=255)
tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctg < 1:2254137/38‑1 (MQ=255)
gaGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCgg < 1:1323498/47‑1 (MQ=255)
gAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg < 1:2703189/70‑2 (MQ=255)
cgTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTgccgccg < 1:3049470/71‑2 (MQ=255)
gtgtTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTgccgccg < 1:1691351/70‑2 (MQ=255)
ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcg > 1:3358151/1‑71 (MQ=255)
tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCt > 1:2549758/1‑67 (MQ=255)
tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCt > 1:1178129/1‑67 (MQ=255)
ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCt > 1:2443948/1‑71 (MQ=255)
ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCt > 1:117427/1‑71 (MQ=255)
ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCt > 1:1725149/1‑71 (MQ=255)
tttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgc > 1:66361/1‑67 (MQ=255)
tttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCtt > 1:3504993/1‑71 (MQ=255)
ttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCtt < 1:63543/70‑1 (MQ=255)
aCAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:3463334/1‑69 (MQ=255)
aCAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:1779442/1‑69 (MQ=255)
gCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:1115746/71‑1 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:256349/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:688963/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCACCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1528744/1‑70 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTAGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:1630231/1‑71 (MQ=255)
tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACtt < 1:842147/48‑1 (MQ=255)
tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:3298645/1‑71 (MQ=255)
tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:2316786/1‑71 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCg < 1:3010248/70‑1 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGa < 1:96148/71‑1 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTc > 1:3073524/1‑46 (MQ=255)
tCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGtt < 1:1729560/63‑1 (MQ=255)
cACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:1543197/1‑70 (MQ=255)
|
CGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACA > minE/1014590‑1014728
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
