Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,076 |
C→T |
5.7% |
Q331Q (CAG→CAA) ‡ |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,076 | 0 | C | T | 5.7%
| 228.4
/ 2.1
| 69 | Q331Q (CAG→CAA) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (47/18); new base T (2/2); total (49/20) |
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGG > minE/1015007‑1015139
|
tcCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt > 1:691334/1‑71 (MQ=255)
cccGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCt > 1:3036100/1‑70 (MQ=255)
cGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:2917931/1‑71 (MQ=255)
cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3614383/71‑1 (MQ=255)
aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:3142280/1‑71 (MQ=255)
aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:1833381/1‑71 (MQ=255)
gCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTg < 1:2010917/58‑1 (MQ=255)
tCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:66194/1‑71 (MQ=255)
tCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1476044/1‑71 (MQ=255)
ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCGCTGACGGTTGATCTTCGCttt < 1:1265051/70‑1 (MQ=255)
ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:1140110/59‑1 (MQ=255)
ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:284169/59‑1 (MQ=255)
ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1505645/70‑1 (MQ=255)
ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:180891/70‑1 (MQ=255)
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGatat < 1:3536867/70‑1 (MQ=255)
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:951932/71‑1 (MQ=255)
atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:730401/1‑49 (MQ=255)
atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:2805781/1‑49 (MQ=255)
tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:2682209/1‑71 (MQ=255)
ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:1602294/1‑46 (MQ=255)
tGCCTGGATTATGTTCCAGTTTTTCGATCAAGATCCCCTGaag > 1:1031139/1‑41 (MQ=38)
tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:202822/1‑41 (MQ=255)
tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:3570709/1‑41 (MQ=255)
tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:447447/1‑41 (MQ=255)
tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2317259/1‑68 (MQ=255)
gATTATGTTCCAGTTTTTGGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:1421823/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2602334/71‑1 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:463143/1‑70 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3226318/1‑70 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2092071/70‑1 (MQ=255)
ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGAt > 1:1871937/1‑41 (MQ=255)
tgtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag > 1:519123/3‑71 (MQ=255)
tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:1293695/1‑51 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:2833994/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:3240928/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:788041/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3470646/71‑1 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2387993/1‑60 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2123218/1‑58 (MQ=255)
cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:2236462/1‑66 (MQ=255)
cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:1528039/1‑66 (MQ=255)
cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:1390731/42‑1 (MQ=19)
aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:1435428/47‑1 (MQ=255)
aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:1878441/1‑44 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1161778/39‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2638609/39‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa < 1:2873524/71‑1 (MQ=255)
ttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:3178995/65‑1 (MQ=255)
tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:485722/1‑71 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAt < 1:1496480/70‑1 (MQ=255)
tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:2360816/1‑61 (MQ=255)
tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:3622661/1‑71 (MQ=255)
cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg < 1:2870580/62‑1 (MQ=255)
cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:2623169/1‑70 (MQ=255)
gATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATac > 1:2664655/1‑68 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc > 1:2033016/1‑62 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1527639/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:858511/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:3142814/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:790025/1‑71 (MQ=255)
tCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgacc > 1:916533/1‑71 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3496109/1‑37 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3629084/1‑37 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1643423/1‑71 (MQ=255)
aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:1920539/1‑71 (MQ=255)
aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:304036/1‑71 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:519633/1‑35 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1458843/1‑39 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2240153/1‑59 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2147126/1‑40 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:251179/1‑40 (MQ=255)
|
TCCCGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGG > minE/1015007‑1015139
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
