Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,097 |
G→A |
5.8% |
I324I (ATC→ATT) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,097 | 0 | G | A | 5.8%
| 224.5
/ 3.1
| 69 | I324I (ATC→ATT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (43/22); new base A (2/2); total (45/24) |
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
ATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCA > minE/1015032‑1015161
|
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGatat < 1:3536867/70‑1 (MQ=255)
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:951932/71‑1 (MQ=255)
tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:2682209/1‑71 (MQ=255)
tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2317259/1‑68 (MQ=255)
gATTATGTTCCAGTTTTTGGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:1421823/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2602334/71‑1 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:463143/1‑70 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3226318/1‑70 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2092071/70‑1 (MQ=255)
tgtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag > 1:519123/3‑71 (MQ=255)
tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:1293695/1‑51 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:3240928/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:788041/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:2833994/1‑69 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3470646/71‑1 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2387993/1‑60 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2123218/1‑58 (MQ=255)
cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:1528039/1‑66 (MQ=255)
cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:2236462/1‑66 (MQ=255)
aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:1435428/47‑1 (MQ=255)
aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:1878441/1‑44 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa < 1:2873524/71‑1 (MQ=255)
ttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:3178995/65‑1 (MQ=255)
tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:485722/1‑71 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAt < 1:1496480/70‑1 (MQ=255)
tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:2360816/1‑61 (MQ=255)
tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:3622661/1‑71 (MQ=255)
cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg < 1:2870580/62‑1 (MQ=255)
cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:2623169/1‑70 (MQ=255)
gATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATac > 1:2664655/1‑68 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc > 1:2033016/1‑62 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:790025/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1527639/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:858511/1‑71 (MQ=255)
aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:3142814/1‑71 (MQ=255)
tCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgacc > 1:916533/1‑71 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3629084/1‑37 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3496109/1‑37 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1643423/1‑71 (MQ=255)
aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:1920539/1‑71 (MQ=255)
aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:304036/1‑71 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:519633/1‑35 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1458843/1‑39 (MQ=255)
tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2240153/1‑59 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:251179/1‑40 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2147126/1‑40 (MQ=255)
tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga > 1:2903237/1‑58 (MQ=255)
tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga > 1:1248502/1‑58 (MQ=255)
tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:943717/1‑71 (MQ=255)
ggTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:653342/1‑66 (MQ=255)
ggTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCg > 1:1964882/1‑71 (MQ=255)
gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt < 1:1594562/71‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga < 1:1819366/53‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga < 1:2799486/53‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga < 1:1562135/53‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:1409692/66‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc < 1:1999935/66‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGcc < 1:973160/69‑1 (MQ=255)
gTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGcc < 1:3526707/69‑1 (MQ=255)
ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:3225770/1‑40 (MQ=255)
ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:1798943/1‑70 (MQ=255)
ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:1145489/1‑70 (MQ=255)
tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt < 1:1945554/69‑1 (MQ=255)
cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac < 1:3016320/48‑1 (MQ=255)
cTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTg > 1:2465474/1‑71 (MQ=255)
ttCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAgg < 1:1187418/55‑1 (MQ=255)
cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg < 1:3726445/69‑1 (MQ=255)
cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTgg < 1:356847/69‑1 (MQ=255)
gCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:2406213/70‑1 (MQ=255)
|
ATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCA > minE/1015032‑1015161
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
