Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R2 370 72.1 3738297 91.5% 3420541 62.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,038,735 G→C 59.8% V73L (GTG→CTG)  rsxE → predicted inner membrane NADH‑quinone reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,038,7350GC59.8% 69.0 / 121.9 107V73L (GTG→CTG) rsxEpredicted inner membrane NADH‑quinone reductase
Reads supporting (aligned to +/- strand):  ref base G (24/19);  new base C (20/44);  total (44/63)
Fisher's exact test for biased strand distribution p-value = 1.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ACGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG  >  minE/1038666‑1038800
                                                                     |                                                                 
aCGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGt                                                                  <  1:2871308/71‑1 (MQ=255)
 cGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGt                                                                  >  1:1060345/1‑70 (MQ=255)
   cTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat                                                               <  1:1763939/71‑1 (MQ=255)
    tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGtgat                                                               >  1:2699325/1‑70 (MQ=255)
    tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat                                                               >  1:2114961/1‑70 (MQ=255)
    tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat                                                               >  1:1812740/1‑70 (MQ=255)
    tGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgat                                                               >  1:3315206/1‑70 (MQ=255)
     gACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgatga                                                             >  1:3384124/1‑71 (MQ=255)
      aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat                                                            >  1:1819989/1‑71 (MQ=255)
      aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat                                                            >  1:582678/1‑71 (MQ=255)
      aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat                                                            >  1:493822/1‑71 (MQ=255)
      aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTgatgat                                                            >  1:2442905/1‑71 (MQ=255)
           ccTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCg                                                       >  1:433561/1‑71 (MQ=255)
           ccTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCg                                                       >  1:1744135/1‑71 (MQ=255)
            cTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGc                                                      >  1:3614882/1‑71 (MQ=255)
            cTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGc                                                      >  1:1249188/1‑71 (MQ=255)
              gACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCt                                                    >  1:3634522/1‑71 (MQ=255)
               aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTc                                                   >  1:3158658/1‑71 (MQ=255)
               aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCt                                                    >  1:945309/1‑70 (MQ=255)
                 cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCgg                                                 <  1:1170167/71‑1 (MQ=255)
                 cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCgg                                                 <  1:2877260/71‑1 (MQ=255)
                 cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:2627061/71‑1 (MQ=255)
                 cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:2030960/71‑1 (MQ=255)
                 cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:2018862/71‑1 (MQ=255)
                  aTTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggt                                                <  1:3456884/71‑1 (MQ=255)
                   tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg                                               >  1:2340059/1‑71 (MQ=255)
                   tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg                                               >  1:2234767/1‑71 (MQ=255)
                   tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg                                               >  1:2709524/1‑71 (MQ=255)
                   tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtg                                               >  1:2136135/1‑71 (MQ=255)
                   tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCg                                                  <  1:1771481/68‑1 (MQ=255)
                      cGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCggtggt                                             <  1:2332988/70‑1 (MQ=255)
                      cGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt                                             <  1:3044074/70‑1 (MQ=255)
                       gACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTc                                            <  1:3177337/70‑1 (MQ=255)
                       gACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTc                                            <  1:598320/70‑1 (MQ=255)
                        aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                <  1:1478488/49‑1 (MQ=255)
                        aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                <  1:866229/49‑1 (MQ=255)
                        aCGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                <  1:1192201/49‑1 (MQ=255)
                          gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt                                             <  1:114355/66‑1 (MQ=255)
                          gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg                                        <  1:1991488/71‑1 (MQ=255)
                          gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg                                        <  1:615538/71‑1 (MQ=255)
                             gCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     >  1:3222801/1‑71 (MQ=255)
                                tCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTAc                                  <  1:54403/71‑1 (MQ=255)
                                 cACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTa                                   >  1:2113378/1‑69 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa                                 <  1:484980/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa                                 <  1:1684116/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa                                 <  1:40407/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa                                 <  1:2837403/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg                                <  1:2705240/71‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGGTGTACa                                 <  1:5394/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACa                                 <  1:82378/70‑1 (MQ=255)
                                  aCTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg                                <  1:3595778/71‑1 (MQ=255)
                                   cTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg                                <  1:2279754/70‑1 (MQ=255)
                                      gACGCCAGCGGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt                              <  1:189393/69‑1 (MQ=255)
                                         gCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGa                         <  1:2086589/71‑1 (MQ=255)
                                          ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        >  1:1940629/1‑71 (MQ=255)
                                          ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        >  1:1549743/1‑71 (MQ=255)
                                          ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        >  1:3164054/1‑71 (MQ=255)
                                           cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       <  1:3348732/71‑1 (MQ=255)
                                           cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       <  1:2415364/71‑1 (MQ=255)
                                           cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       <  1:1780661/71‑1 (MQ=255)
                                            aGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCa                      <  1:609814/71‑1 (MQ=255)
                                            aGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCa                      <  1:2255450/71‑1 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa                     >  1:879754/1‑70 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:3154803/71‑1 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    >  1:1099785/1‑71 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    >  1:1669324/1‑71 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:2861825/71‑1 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:1384132/71‑1 (MQ=255)
                                              ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:2757765/71‑1 (MQ=255)
                                                  gaTCCGCATTCCCATTTACGTGATGATCATCGCCTCggtggt                                             <  1:2781840/42‑1 (MQ=255)
                                                  gaTCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc                 >  1:1733212/1‑70 (MQ=255)
                                                    tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       >  1:3668410/1‑62 (MQ=255)
                                                    tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:505073/1‑70 (MQ=255)
                                                    tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:1568368/1‑70 (MQ=255)
                                                     ccGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctacg             >  1:1475089/1‑71 (MQ=255)
                                                      cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc                 <  1:3254201/66‑1 (MQ=255)
                                                      cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc                 <  1:3519515/66‑1 (MQ=255)
                                                      cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc                 <  1:3070681/66‑1 (MQ=255)
                                                      cGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcc                 <  1:556566/66‑1 (MQ=255)
                                                        cATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctacgcct          <  1:868664/71‑1 (MQ=255)
                                                         aTTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         >  1:2817901/1‑71 (MQ=255)
                                                         aTTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         >  1:1032804/1‑71 (MQ=255)
                                                         aTTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         <  1:535884/71‑1 (MQ=255)
                                                          ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa                     >  1:3524860/1‑58 (MQ=255)
                                                          ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa                     >  1:1159615/1‑58 (MQ=255)
                                                          ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         <  1:3305161/70‑1 (MQ=255)
                                                          ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         <  1:1571124/70‑1 (MQ=255)
                                                             ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        <  1:381315/52‑1 (MQ=255)
                                                             ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCtt         <  1:2599601/67‑1 (MQ=255)
                                                             ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc     <  1:1039573/71‑1 (MQ=255)
                                                             ccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc     <  1:3505055/71‑1 (MQ=255)
                                                              cATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGc     <  1:1312694/70‑1 (MQ=255)
                                                               aTTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       <  1:2023610/51‑1 (MQ=255)
                                                               aTTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATc                       <  1:1339554/51‑1 (MQ=255)
                                                                tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:2949435/1‑58 (MQ=255)
                                                                tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:1888100/1‑58 (MQ=255)
                                                                tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:1179997/1‑58 (MQ=255)
                                                                tttACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg  >  1:3255312/1‑71 (MQ=255)
                                                                tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGa                         <  1:947949/48‑1 (MQ=255)
                                                                tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg  <  1:3601808/71‑1 (MQ=255)
                                                                     |                                                                 
ACGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG  >  minE/1038666‑1038800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A