Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,091,167 |
T→C |
52.1% |
E178E (GAA→GAG) |
sufB ← |
component of SufBCD complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,091,167 | 0 | T | C | 52.1%
| 1.5
/ 67.1
| 48 | E178E (GAA→GAG) | sufB | component of SufBCD complex |
| Reads supporting (aligned to +/- strand): ref base T (12/11); new base C (15/10); total (27/21) |
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 |
CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAT > minE/1091100‑1091237
|
ccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTcc < 1:2071346/71‑1 (MQ=255)
gcATTAAGCGCGTCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg < 1:2060041/71‑1 (MQ=255)
gcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCgg < 1:2625242/71‑1 (MQ=255)
gcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg < 1:1359093/71‑1 (MQ=255)
aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGAt > 1:676362/1‑71 (MQ=255)
aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGAt > 1:225160/1‑71 (MQ=255)
cgcgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGt < 1:2702694/71‑1 (MQ=255)
cgcgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGt < 1:1617315/71‑1 (MQ=255)
cAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATc < 1:2078114/71‑1 (MQ=255)
cAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATc < 1:1978844/71‑1 (MQ=255)
agaagTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGcc > 1:3530986/1‑71 (MQ=255)
tGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCAcc > 1:2514481/1‑70 (MQ=255)
gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCg > 1:2676701/1‑55 (MQ=255)
gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCgg > 1:1744903/1‑48 (MQ=255)
tCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGAt < 1:1047754/52‑1 (MQ=255)
aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAgg < 1:1419729/71‑1 (MQ=255)
aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGAt < 1:3503460/57‑1 (MQ=255)
aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAg < 1:2451826/70‑1 (MQ=255)
ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCa > 1:1770516/1‑63 (MQ=255)
ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCa > 1:1812430/1‑63 (MQ=255)
ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGa < 1:1128103/71‑1 (MQ=255)
cGGCACCGTGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAGCAgg > 1:2015725/1‑70 (MQ=255)
cGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGa < 1:1068632/71‑1 (MQ=255)
caccacGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaaaa < 1:2514225/71‑1 (MQ=255)
gtggTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCaa < 1:3203599/53‑1 (MQ=255)
acGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataat > 1:494505/1‑71 (MQ=255)
cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt > 1:1030784/1‑71 (MQ=255)
ggTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCAcc > 1:1490792/1‑51 (MQ=255)
ggTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTc > 1:1011953/1‑71 (MQ=255)
tGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc < 1:1328921/57‑1 (MQ=255)
tGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTccc < 1:3258934/71‑1 (MQ=255)
cGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc < 1:2494855/54‑1 (MQ=255)
gagaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCt > 1:3232024/1‑71 (MQ=255)
gagaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCt > 1:535695/1‑71 (MQ=255)
gaTATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTcc > 1:930958/1‑71 (MQ=255)
gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:2012162/1‑51 (MQ=255)
gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:366854/1‑51 (MQ=255)
gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc > 1:1761208/1‑51 (MQ=255)
gaTATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTcc > 1:974280/1‑71 (MQ=255)
atatTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg > 1:231193/1‑71 (MQ=255)
tttACGCACCAGCTCCGGGTGATCGTGGATCGCCTCa > 1:3258570/1‑37 (MQ=255)
tACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGt > 1:43620/1‑71 (MQ=255)
aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:3016528/1‑40 (MQ=255)
aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:3304539/1‑40 (MQ=255)
aCGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg > 1:836133/1‑40 (MQ=255)
gCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGtttt < 1:344388/71‑1 (MQ=255)
ccAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt < 1:2719848/50‑1 (MQ=255)
cAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc > 1:11333/1‑71 (MQ=255)
ttCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt < 1:3595851/71‑1 (MQ=255)
ttCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt < 1:1907374/71‑1 (MQ=255)
|
CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAT > minE/1091100‑1091237
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
