Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,187,540 |
G→A |
34.9% |
G10R (GGG→AGG) |
yeaI → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,187,540 | 0 | G | A | 34.9%
| 44.3
/ 39.2
| 43 | G10R (GGG→AGG) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (12/16); new base A (6/9); total (18/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCT > minE/1187471‑1187604
|
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATggg < 1:2343599/71‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATggg < 1:1911940/71‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATgg < 1:2600082/70‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATgg < 1:153846/70‑1 (MQ=255)
cTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGt < 1:1917963/71‑1 (MQ=255)
aaTTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCtt > 1:581265/1‑70 (MQ=255)
aTTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCttt < 1:14028/70‑1 (MQ=255)
ccAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAttt < 1:3433460/70‑1 (MQ=255)
cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt < 1:2327924/70‑1 (MQ=255)
cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt < 1:3545269/70‑1 (MQ=255)
cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt < 1:3455125/70‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:1749685/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:2565536/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:3128597/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:1896080/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:1569540/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:1126344/54‑1 (MQ=255)
aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGCTTa < 1:1387982/54‑1 (MQ=255)
aaaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTAt < 1:898574/70‑1 (MQ=255)
aaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg > 1:2159134/1‑71 (MQ=255)
aaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg < 1:2747606/71‑1 (MQ=255)
aCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg < 1:582071/70‑1 (MQ=255)
aCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa < 1:1421674/49‑1 (MQ=255)
aaTCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTa < 1:1854261/71‑1 (MQ=255)
cATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtt < 1:1732225/57‑1 (MQ=255)
tCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGaa < 1:934417/70‑1 (MQ=255)
aaaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa > 1:1774024/1‑71 (MQ=255)
aaaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa < 1:1186799/71‑1 (MQ=255)
aaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa > 1:2910027/1‑70 (MQ=255)
aaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGAt > 1:1893100/1‑71 (MQ=255)
tGATTCAGTCAACACGTATTTCCATGGGGTTATTCt > 1:1159983/1‑36 (MQ=255)
tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt > 1:2501842/1‑37 (MQ=255)
tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt > 1:2104378/1‑37 (MQ=255)
tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt > 1:1554628/1‑37 (MQ=255)
tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt > 1:1168397/1‑37 (MQ=255)
aGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg < 1:1846575/47‑1 (MQ=255)
aGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCaa < 1:1529208/71‑1 (MQ=255)
tCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGtt > 1:811984/1‑47 (MQ=255)
tCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGtt > 1:881547/1‑47 (MQ=255)
acacGTATTTCCATGAGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACta < 1:1513902/71‑1 (MQ=255)
cacGTATTTCCATGAGGTTATTCTTTAAATAtttttt > 1:912957/1‑37 (MQ=255)
acGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATc > 1:93423/1‑56 (MQ=255)
gTATTTCCATGAGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACtatata > 1:3296496/1‑71 (MQ=255)
tttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACt < 1:1249647/63‑1 (MQ=255)
tttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATAtct > 1:1307647/1‑71 (MQ=255)
ttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATAtctc > 1:3428896/1‑71 (MQ=255)
tCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATctct > 1:1927544/1‑71 (MQ=255)
|
ACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCT > minE/1187471‑1187604
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
