Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I0 R2	370	72.1	3738297	91.5%	3420541	62.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,259,349	A→C	6.9%	I525M (ATT→ATG) ‡	torZ ←	trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,259,349	0	A	C	6.9%	186.2 / 9.0	73	I525M (ATT→ATG) ‡	torZ	trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand): ref base A (24/44); new base C (3/2); total (27/46)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

AAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAA  >  minE/1259280‑1259417

aaGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa                                                                     >  1:3409824/1‑71 (MQ=255)

      ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGc                                                                <  1:993649/70‑1 (MQ=255)

        cAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTgg                                                             >  1:748662/1‑71 (MQ=255)

        cAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCt                                                               <  1:2059905/69‑1 (MQ=255)

        cAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCt                                                               <  1:1562751/69‑1 (MQ=255)

        cAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTga                                                             >  1:2350454/1‑70 (MQ=255)

           acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGtt                                                           >  1:2282913/1‑70 (MQ=255)

            cacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc                                                         <  1:2585506/71‑1 (MQ=255)

               gTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTg                                                       >  1:589862/1‑70 (MQ=255)

                 cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa                                                    >  1:2014280/1‑71 (MQ=255)

                    aaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCa                                                 >  1:2416350/1‑71 (MQ=255)

                    aaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCa                                                 <  1:1808705/71‑1 (MQ=255)

                     aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCa                                                 <  1:3049339/70‑1 (MQ=255)

                     aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCa                                                 <  1:3027357/70‑1 (MQ=255)

                     aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc                                                >  1:907653/1‑71 (MQ=255)

                     aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc                                                <  1:1884193/71‑1 (MQ=255)

                      tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc                                               <  1:1971258/71‑1 (MQ=255)

                        gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                              <  1:400416/70‑1 (MQ=255)

                           aCGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCa                                          >  1:1349794/1‑71 (MQ=255)

                              cgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCg                                       <  1:3067759/71‑1 (MQ=255)

                                 ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc                                     >  1:1062939/1‑70 (MQ=255)

                                    aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                                 >  1:2369810/1‑71 (MQ=255)

                                    aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                                 >  1:1570184/1‑71 (MQ=255)

                                    aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                                 >  1:3256275/1‑71 (MQ=255)

                                    aaaTTGAGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                                 >  1:1001083/1‑71 (MQ=255)

                                     aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa                                                     <  1:3103699/50‑1 (MQ=255)

                                     aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa                                                     <  1:3222856/50‑1 (MQ=255)

                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:3577537/71‑1 (MQ=255)

                                          cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc                           >  1:3581823/1‑71 (MQ=255)

                                             aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                                      <  1:1525788/57‑1 (MQ=255)

                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:1903839/62‑1 (MQ=255)

                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:2989869/62‑1 (MQ=255)

                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:2776741/62‑1 (MQ=255)

                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:1822957/62‑1 (MQ=255)

                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                               <  1:1368554/62‑1 (MQ=255)

                                                aaCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                     <  1:119038/71‑1 (MQ=255)

                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                    >  1:3193312/1‑71 (MQ=255)

                                                  cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                   <  1:2683905/71‑1 (MQ=255)

                                                   aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                   >  1:1480278/1‑70 (MQ=255)

                                                      cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg               <  1:1348972/71‑1 (MQ=255)

                                                      cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg               <  1:1837008/71‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                        >  1:598934/1‑45 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:924938/53‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:841889/53‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:2918853/53‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:2881352/53‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:447234/53‑1 (MQ=255)

                                                       tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:2295012/53‑1 (MQ=255)

                                                        gCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGt                                      >  1:993188/1‑46 (MQ=255)

                                                        gCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGt                                      >  1:3194484/1‑46 (MQ=255)

                                                        gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                    <  1:2627535/64‑1 (MQ=255)

                                                        gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                    <  1:1419284/64‑1 (MQ=255)

                                                        gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                    <  1:2335830/64‑1 (MQ=255)

                                                         cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg             <  1:82044/70‑1 (MQ=255)

                                                         cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt              <  1:271044/69‑1 (MQ=255)

                                                          ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt           >  1:3387046/1‑71 (MQ=255)

                                                          ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt           >  1:2932994/1‑71 (MQ=255)

                                                            cATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGa         >  1:170568/1‑71 (MQ=255)

                                                            cATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGa         >  1:2452701/1‑71 (MQ=255)

                                                            cATCGGCACAATATGCTGGTTGATGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGa         >  1:3225044/1‑71 (MQ=255)

                                                             aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGa         >  1:1353028/1‑70 (MQ=255)

                                                             aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt        <  1:2543152/71‑1 (MQ=255)

                                                             aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt        >  1:740711/1‑71 (MQ=255)

                                                             aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt        <  1:787420/71‑1 (MQ=255)

                                                             aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt        <  1:3030026/71‑1 (MQ=255)

                                                               cGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg      >  1:3719212/1‑71 (MQ=255)

                                                                ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg     <  1:800021/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:371297/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:1044946/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:514938/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:2376862/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:2164818/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:78401/71‑1 (MQ=255)

                                                                 gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt    <  1:181228/71‑1 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAg                                  <  1:1327000/38‑1 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAg                                  <  1:3137492/38‑1 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                                <  1:1522335/40‑1 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa  >  1:970728/1‑70 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa  >  1:2208639/1‑70 (MQ=255)

                                                                    caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa  >  1:2526954/1‑70 (MQ=255)

AAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAA  >  minE/1259280‑1259417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A