Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,429,321 |
T→C |
39.2% |
M88V (ATG→GTG) |
wzzE ← |
Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,429,321 | 0 | T | C | 39.2%
| 56.5
/ 90.2
| 79 | M88V (ATG→GTG) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base T (25/23); new base C (19/12); total (44/35) |
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 |
GCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGTT > minE/2429257‑2429391
|
gCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTg < 1:547316/70‑1 (MQ=255)
cAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAAc < 1:2622734/71‑1 (MQ=255)
cAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAAc < 1:2447942/71‑1 (MQ=255)
aGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACg < 1:924303/71‑1 (MQ=255)
aGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACg < 1:185071/71‑1 (MQ=255)
ttGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAAc < 1:2895678/68‑1 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:2504740/1‑68 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3315960/1‑68 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3500917/1‑68 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:3525196/1‑68 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACgg > 1:540473/1‑68 (MQ=255)
gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa > 1:1263212/1‑71 (MQ=255)
aTAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATc < 1:714988/71‑1 (MQ=255)
tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATcc < 1:3246396/71‑1 (MQ=255)
tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATc > 1:31935/1‑70 (MQ=255)
tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATcc < 1:3119291/71‑1 (MQ=255)
tAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATcc < 1:2455715/71‑1 (MQ=255)
aCAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACg > 1:1346540/1‑62 (MQ=255)
cAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCAt > 1:1156060/1‑52 (MQ=255)
cAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAgg < 1:1849367/71‑1 (MQ=255)
aaaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:1696006/1‑71 (MQ=255)
aaaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa > 1:3593754/1‑64 (MQ=255)
aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:3556421/1‑70 (MQ=255)
aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGt > 1:644498/1‑70 (MQ=255)
aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTa < 1:3196334/71‑1 (MQ=255)
aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTa > 1:1944787/1‑71 (MQ=255)
aCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:1759875/1‑71 (MQ=255)
ctctTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTAc < 1:3303564/71‑1 (MQ=255)
ctctTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTAc > 1:834540/1‑71 (MQ=255)
tttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGc > 1:2010807/1‑70 (MQ=255)
ttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCa > 1:1551769/1‑70 (MQ=255)
tataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa > 1:3559689/1‑70 (MQ=255)
ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGtt > 1:1630024/1‑45 (MQ=255)
ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGtt > 1:1584301/1‑45 (MQ=255)
ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaa > 1:704229/1‑70 (MQ=255)
taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:1812497/1‑71 (MQ=255)
taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:708006/1‑71 (MQ=255)
taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaaa < 1:2379172/70‑1 (MQ=255)
ggCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAAt < 1:3306147/70‑1 (MQ=255)
gCTTCGTCCATGACC‑ATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:3628642/71‑1 (MQ=255)
cTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:2915930/71‑1 (MQ=255)
cTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgc < 1:2384419/71‑1 (MQ=255)
ttCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgct > 1:3555059/1‑71 (MQ=255)
ggccATGACCGATGGGTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:3533881/69‑1 (MQ=255)
gTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:644628/71‑1 (MQ=255)
gTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct < 1:1325029/71‑1 (MQ=255)
ccATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:2673022/1‑53 (MQ=255)
ccATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa > 1:2687893/1‑53 (MQ=255)
cATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgctgc > 1:3116106/1‑70 (MQ=255)
cATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCg < 1:2207106/71‑1 (MQ=255)
aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:2757347/1‑54 (MQ=255)
aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:3713097/1‑54 (MQ=255)
aTGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGc > 1:2131458/1‑54 (MQ=255)
tGACCGATGGTTGATCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa > 1:2770613/1‑55 (MQ=255)
gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1499991/1‑70 (MQ=255)
gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1880210/1‑70 (MQ=255)
gACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg > 1:1747700/1‑70 (MQ=255)
aCCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGt < 1:567342/70‑1 (MQ=255)
aCCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGt < 1:3116165/70‑1 (MQ=255)
aCCGATGGTTGGACGGCAGAAGCCATGTTTGAACgg < 1:583762/36‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:354504/42‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2365807/42‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:339099/42‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:3649841/42‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2757902/42‑1 (MQ=255)
gtCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCaa < 1:2105912/42‑1 (MQ=255)
gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTa < 1:562441/60‑1 (MQ=255)
gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc > 1:3438588/1‑69 (MQ=255)
gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc > 1:2553210/1‑69 (MQ=255)
ggCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCa > 1:3049481/1‑71 (MQ=255)
gCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCa > 1:14983/1‑70 (MQ=255)
cAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata < 1:1961737/71‑1 (MQ=255)
cAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata > 1:972938/1‑71 (MQ=255)
gAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGa < 1:3232260/50‑1 (MQ=255)
aaGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCAGCGAGTAATATCCCCCCAGCATATTc < 1:647217/71‑1 (MQ=255)
gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:2122894/1‑35 (MQ=255)
gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:2377163/1‑35 (MQ=255)
gCCACGTTTGAACGGACATCCAGGTTACGCaaaaa > 1:3249031/1‑35 (MQ=255)
aTGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGt > 1:2420187/1‑71 (MQ=255)
aTGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGt > 1:2457498/1‑71 (MQ=255)
tGTTTGAAGGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGtt > 1:3131841/1‑71 (MQ=255)
|
GCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACCGTT > minE/2429257‑2429391
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
