Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,823,561 |
A→C |
60.6% |
intergenic (+306/+652) |
nrfG → / ← pheU |
heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,823,561 | 0 | A | C | 60.6%
| 139.7
/ 100.2
| 109 | intergenic (+306/+652) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (15/28); new base C (26/40); total (41/68) |
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 120 total aligned reads displayed.
TTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > minE/2823492‑2823627
|
aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc < 1:1570985/70‑1 (MQ=255)
aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:1036278/71‑1 (MQ=255)
aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:196280/71‑1 (MQ=255)
aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:1953000/71‑1 (MQ=255)
aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:426767/1‑70 (MQ=255)
aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:1523505/71‑1 (MQ=255)
aaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa < 1:459520/71‑1 (MQ=255)
aaaTCTACAAACCAACGCAACACAATTCATGCCCAGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:3275463/1‑69 (MQ=255)
aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa < 1:2489265/70‑1 (MQ=255)
aTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAAc > 1:2913080/1‑71 (MQ=255)
tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc < 1:1174270/61‑1 (MQ=255)
tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg < 1:2206791/64‑1 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:3434228/68‑1 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:2557499/68‑1 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:3130077/68‑1 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc < 1:3179479/71‑1 (MQ=255)
aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTc > 1:473221/1‑70 (MQ=255)
aCCAACGCAACACAATTCATGCACTGGCAGTATGTCACGTTCTCGCGTTTCTCaa > 1:898567/1‑55 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:3590782/1‑52 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1056391/1‑52 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:1928345/71‑1 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:2664479/71‑1 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:3331176/71‑1 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:3149285/1‑51 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:916323/1‑51 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1544231/1‑51 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTg < 1:580328/71‑1 (MQ=255)
gCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg < 1:3108039/71‑1 (MQ=255)
gCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg > 1:798001/1‑51 (MQ=255)
aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCt > 1:1356321/1‑61 (MQ=255)
aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCt > 1:2136624/1‑61 (MQ=255)
aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAgg > 1:3133376/1‑70 (MQ=255)
acacAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:3683298/49‑1 (MQ=255)
acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgggg > 1:3270017/1‑49 (MQ=255)
acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgggg > 1:985826/1‑49 (MQ=255)
acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:1032228/1‑48 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGa < 1:2094606/66‑1 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGa < 1:2002410/66‑1 (MQ=255)
aTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:876964/44‑1 (MQ=255)
ttCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:2125330/1‑71 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:2145697/1‑39 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:268267/1‑39 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:687826/1‑39 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1297122/1‑39 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:845450/62‑1 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGATCGGGGAACGGCGCTCCATTGa < 1:1304714/60‑1 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGa < 1:2405933/60‑1 (MQ=255)
tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGa < 1:2730958/60‑1 (MQ=255)
aTGCCCTGGCAGTATGTCACGTTCTCGGGTTTCTGCACGGGGAACGGCGCTCCATTGAGGaa > 1:2283553/1‑62 (MQ=255)
aTGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGaa > 1:3715896/1‑62 (MQ=255)
aTGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcattcat < 1:2212346/71‑1 (MQ=255)
gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:1862397/50‑1 (MQ=255)
gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:1494944/50‑1 (MQ=255)
cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1394440/1‑70 (MQ=255)
cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1081670/1‑70 (MQ=255)
ccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcattca < 1:2670769/66‑1 (MQ=255)
ccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:3281668/1‑69 (MQ=255)
ccTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:533356/1‑69 (MQ=255)
tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACgg < 1:92054/41‑1 (MQ=255)
tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg > 1:393815/1‑51 (MQ=255)
tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg > 1:1204238/1‑51 (MQ=255)
ggCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa > 1:2431735/1‑47 (MQ=255)
ggCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa > 1:2359772/1‑70 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:964902/1‑60 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca < 1:2897445/58‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGaa < 1:2209736/70‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:736684/69‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:1827862/69‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGt < 1:2594546/56‑1 (MQ=255)
gTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtc < 1:864108/54‑1 (MQ=255)
gTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtc < 1:830625/54‑1 (MQ=255)
tATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCa < 1:291537/71‑1 (MQ=255)
tATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCa < 1:2854270/71‑1 (MQ=255)
aTGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAt > 1:2561140/1‑71 (MQ=255)
gTCACGTTCTCGCGTTTCTGCACGTGGAACGGCGCTCCATTGAGGAAGtc < 1:2616992/50‑1 (MQ=255)
cACGTTCTCGCGTTTGTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:2176642/1‑50 (MQ=255)
cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:2271518/1‑50 (MQ=255)
cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:1073937/1‑50 (MQ=255)
cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:1189566/1‑50 (MQ=255)
cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:416504/1‑49 (MQ=255)
cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg < 1:2308150/59‑1 (MQ=255)
aCGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaa < 1:69990/71‑1 (MQ=255)
cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:1646152/71‑1 (MQ=255)
cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:1738819/71‑1 (MQ=255)
gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaaa < 1:151660/71‑1 (MQ=255)
gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaaa < 1:2682894/71‑1 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:196758/39‑1 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAg < 1:2463737/71‑1 (MQ=255)
ctcGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:1985728/1‑70 (MQ=255)
ctcGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:2573960/1‑70 (MQ=255)
gcgTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAggg < 1:2072724/68‑1 (MQ=255)
gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:1243953/1‑71 (MQ=255)
gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:1265365/1‑71 (MQ=255)
gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:2173912/1‑71 (MQ=255)
tCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatgatg < 1:3364975/70‑1 (MQ=255)
cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg > 1:842225/1‑44 (MQ=255)
cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc < 1:2663418/70‑1 (MQ=255)
cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc < 1:1964246/70‑1 (MQ=255)
tGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata < 1:2612864/39‑1 (MQ=255)
tGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata < 1:2495626/39‑1 (MQ=255)
|
TTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > minE/2823492‑2823627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
