Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,823,561 |
A→C |
intergenic (+306/+652) |
nrfG → / ← pheU |
heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,823,561 | 0 | A | C | 100.0%
| 304.8
/ NA
| 82 | intergenic (+306/+652) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (42/40); total (42/40) |
AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > minE/2823494‑2823627
|
aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc > 1:1070739/1‑70 (MQ=255)
aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc > 1:197823/1‑70 (MQ=255)
aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc > 1:68122/1‑70 (MQ=255)
aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc > 1:2914839/1‑70 (MQ=255)
aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:3180138/71‑1 (MQ=255)
aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgggg < 1:2977473/71‑1 (MQ=255)
aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc < 1:2227090/65‑1 (MQ=255)
aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa < 1:1609463/71‑1 (MQ=255)
aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa < 1:2510090/71‑1 (MQ=255)
aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa < 1:2529280/71‑1 (MQ=255)
tCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAAc > 1:1686007/1‑70 (MQ=255)
tCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACg > 1:1486597/1‑71 (MQ=255)
tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGgc > 1:247020/1‑71 (MQ=255)
tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGgc > 1:761760/1‑71 (MQ=255)
tACAAAACAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGgc > 1:411571/1‑71 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACg < 1:1038682/67‑1 (MQ=255)
cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACg < 1:2374111/67‑1 (MQ=255)
aaCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTc > 1:1865893/1‑71 (MQ=255)
cAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTcc > 1:1741086/1‑69 (MQ=255)
cAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAt > 1:3117562/1‑71 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTcc < 1:389160/68‑1 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTcc < 1:518679/68‑1 (MQ=255)
aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:2047172/71‑1 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg < 1:2289737/71‑1 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg < 1:297510/71‑1 (MQ=255)
aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg < 1:831/71‑1 (MQ=255)
cGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTgt < 1:66878/71‑2 (MQ=255)
cAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:1495402/67‑1 (MQ=255)
cAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:194292/1‑70 (MQ=255)
acacAATTCATGCCCTGGCAGTATCTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg > 1:1294841/1‑69 (MQ=255)
cacaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAg > 1:58262/1‑71 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:108365/1‑71 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca < 1:1292833/71‑1 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:1074635/1‑71 (MQ=255)
aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:1312729/1‑71 (MQ=255)
tGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata < 1:219588/71‑1 (MQ=255)
cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1752741/1‑70 (MQ=255)
cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1970038/1‑70 (MQ=255)
ccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATT‑AGGAAGTCATTCataa > 1:564364/1‑68 (MQ=255)
cTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:61903/1‑68 (MQ=255)
tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCa < 1:2238524/48‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:341522/69‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:1368784/69‑1 (MQ=255)
gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:143987/69‑1 (MQ=255)
cAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa > 1:1502577/1‑35 (MQ=255)
cAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa > 1:390926/1‑35 (MQ=255)
cAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa > 1:2649859/1‑35 (MQ=255)
cAGTATGTCACGTTCTCGCGTTTCTGCACGGGGaa > 1:741206/1‑35 (MQ=255)
aGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGgcgc < 1:1727228/40‑1 (MQ=255)
aTGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcattca > 1:345137/1‑57 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTc < 1:2650176/36‑1 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:1915101/1‑43 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:3063999/1‑43 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:2850734/1‑43 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:241762/1‑43 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:451345/1‑43 (MQ=255)
tCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCg < 1:2484160/71‑1 (MQ=255)
tCACGTTCTAGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg > 1:2400107/1‑43 (MQ=255)
aCGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca < 1:998794/48‑1 (MQ=255)
aCGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaa < 1:1534777/71‑1 (MQ=255)
cGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:985402/71‑1 (MQ=255)
gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:2926202/1‑48 (MQ=255)
gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:830935/1‑48 (MQ=255)
gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:1407566/1‑48 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:1249023/39‑1 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:1621195/39‑1 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:1898904/39‑1 (MQ=255)
ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGa < 1:3158657/67‑1 (MQ=255)
tctcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:722396/38‑1 (MQ=255)
tctcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:2927743/1‑71 (MQ=255)
tctcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:2277146/1‑71 (MQ=255)
ctcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:2323747/1‑43 (MQ=255)
tcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:1413764/53‑1 (MQ=255)
tcGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:2692051/53‑1 (MQ=255)
cgcgTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGaa > 1:1563141/1‑71 (MQ=255)
gcgTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGa < 1:2331576/69‑1 (MQ=255)
gcgTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAat > 1:1556047/1‑71 (MQ=255)
gTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga < 1:1837071/71‑1 (MQ=255)
tCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatgatg < 1:2029679/70‑1 (MQ=255)
cTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc < 1:1174866/70‑1 (MQ=255)
cTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc > 1:387710/1‑70 (MQ=255)
cTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAATCGAAAAGGGAATGATGc < 1:445153/70‑1 (MQ=255)
|
AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > minE/2823494‑2823627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
