mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 13,094 | A→T | D311V (GAT→GTT) | dnaK → | chaperone Hsp70, co‑chaperone with DnaJ |
| RA | 17,199 | G→T | intergenic (+3/‑57) | nhaA → / → nhaR | sodium‑proton antiporter/DNA‑binding transcriptional activator |
| RA | 21,090 | A→G | D341G (GAC→GGC) | ileS → | isoleucyl‑tRNA synthetase |
| RA | 62,679 | A→G | F260F (TTT→TTC) | polB ← | DNA polymerase II |
| RA | 120,663 | C→T | C181Y (TGC→TAC) | speE ← | spermidine synthase |
| RA | 145,530 | T→A | T233S (ACA→TCA) | sfsA ← | predicted DNA‑binding transcriptional regulator |
| RA | 151,149 | C→T | S560S (AGC→AGT) | mrcB → | fused glycosyl transferase and transpeptidase |
| RA | 204,556 | A→G | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| RA | 291,204 | T→C | I274T (ATC→ACC) | ppiD → | peptidyl‑prolyl cis‑trans isomerase |
| RA | 406,585 | A→G | D8D (GAT→GAC) | ybeB ← | hypothetical protein |
| RA | 421,280 | G→A | A400V (GCA→GTA) | asnB ← | asparagine synthetase B |
| RA | 530,638 | T→C | E105E (GAA→GAG) | ybhP ← | predicted DNase |
| RA | 709,001 | A→G | Y102H (TAT→CAT) | yceI ← | hypothetical protein |
| RA | 753,855 | T→C | N189S (AAC→AGC) | ycfT ← | predicted inner membrane protein |
| RA | 780,667 | T→C | D125D (GAT→GAC) | ycgM → | predicted isomerase/hydrolase |
| RA | 801,414 | A→G | S186S (AGT→AGC) | dhaL ← | dihydroxyacetone kinase, C‑terminal domain |
| RA | 826,223 | G→T | G207V (GGT→GTT) | ychP → | predicted invasin |
| RA | 965,646 | C→T | G83D (GGC→GAC) | ydeM ← | conserved hypothetical protein |
| RA | 1,019,797 | T→C | Y249C (TAT→TGT) | uidC ← | predicted outer membrane porin protein |
| RA | 1,036,505 | G→A | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
| RA | 1,038,735 | G→C | V73L (GTG→CTG) | rsxE → | predicted inner membrane NADH‑quinone reductase |
| RA | 1,091,167 | T→C | E178E (GAA→GAG) | sufB ← | component of SufBCD complex |
| RA | 1,093,658 | T→C | T907A (ACA→GCA) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| RA | 1,153,878 | G→A | T246I (ACA→ATA) | astE ← | succinylglutamate desuccinylase |
| RA | 1,216,702 | T→C | Q677Q (CAA→CAG) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| RA | 1,230,834 | +A | coding (1922/2061 nt) | ptrB ← | protease II |
| RA | 1,246,787 | C→T | G70G (GGC→GGT) | znuC → | zinc transporter subunit |
| RA | 1,302,608 | T→C | K158R (AAG→AGG) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
| RA | 1,427,154 | C→T | R220H (CGC→CAC) | napG ← | ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB) |
| RA | 1,434,773 | T→C | A465A (GCA→GCG) | yojI ← | fused predicted multidrug transport subunits and membrane component and ATP‑binding component of ABC superfamily |
| RA | 1,522,743 | T→C | I121V (ATC→GTC) | pdxB ← | erythronate‑4‑phosphate dehydrogenase |
| RA | 1,695,639 | T→C | E124G (GAG→GGG) | yfhQ ← | predicted methyltransferase |
| RA | 1,735,602 | T→C | E157E (GAA→GAG) | era ← | membrane‑associated, 16S rRNA‑binding GTPase |
| RA | 1,738,791 | G→A | C340C (TGC→TGT) | lepA ← | GTP binding membrane protein |
| RA | 1,739,943 | T→C | intergenic (‑133/+65) | lepA ← / ← rseC | GTP binding membrane protein/RseC protein involved in reduction of the SoxR iron‑sulfur cluster |
| RA | 1,744,552 | G→A | V483I (GTC→ATC) | nadB → | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| RA | 1,751,256 | G→A | intergenic (‑42/‑165) | yfiF ← / → trxC | predicted methyltransferase/thioredoxin 2 |
| RA | 1,813,392 | G→A | R595R (CGC→CGT) | alaS ← | alanyl‑tRNA synthetase |
| RA | 1,824,288 | G→A | D452D (GAC→GAT) | hypF ← | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| RA | 1,892,019 | +GC | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| RA | 1,954,781 | Δ1 bp | intergenic (‑69/‑99) | ygfB ← / → zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| RA | 2,012,045 | T→C | M63V (ATG→GTG) | yqhA ← | conserved inner membrane protein |
| RA | 2,207,332 | A→G | intergenic (‑22/+408) | tldD ← / ← yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
| RA | 2,281,121 | A→G | F67S (TTT→TCT) | fabR ← | DNA‑binding transcriptional repressor |
| RA | 2,367,537 | C→T | intergenic (‑85/‑105) | pepQ ← / → fadB | proline dipeptidase/fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| RA | 2,429,321 | T→C | M88V (ATG→GTG) | wzzE ← | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| RA | 2,463,313 | G→A | V200V (GTG→GTA) | gidB → | methyltransferase, glucose‑inhibited cell‑division protein |
| RA | 2,512,108 | C→T | C141Y (TGC→TAC) | yicE ← | predicted transporter |
| RA | 2,523,621 | C→T | G32S (GGT→AGT) | dinD ← | DNA‑damage‑inducible protein |
| RA | 2,526,945 | C→T | E13K (GAG→AAG) | ttk ← | division inhibitor |
| RA | 2,546,001 | C→T | M182I (ATG→ATA) | rfaF ← | ADP‑heptose:LPS heptosyltransferase II |
| RA | 2,550,360 | A→G | K77K (AAA→AAG) | tdh → | threonine 3‑dehydrogenase, NAD(P)‑binding |
| RA | 2,605,853 | T→C | V14V (GTT→GTC) | yhiO → | predicted universal stress (ethanol tolerance) protein B |
| RA | 2,625,149 | G→A | R16H (CGT→CAT) | ftsX → | predicted transporter subunit |
| RA | 2,714,239 | C→A | R41L (CGC→CTC) | nirB ← | nitrite reductase, large subunit, NAD(P)H‑binding |
| RA | 2,727,940 | T→C | F59F (TTT→TTC) | kefB → | potassium:proton antiporter |
| RA | 2,736,671 | T→C | F605F (TTT→TTC) | fusA → | protein chain elongation factor EF‑G |
| RA | 2,811,028 | G→A | A573V (GCA→GTA) | uvrA ← | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| RA | 2,823,561 | A→C | intergenic (+306/+652) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| RA | 2,829,664 | T→C | N109S (AAC→AGC) | aspA ← | aspartate ammonia‑lyase |
| RA | 2,892,177 | G→A | D278N (GAC→AAC) | ytfQ → | predicted sugar transporter subunit |
| RA | 2,895,458 | G→A | A164A (GCG→GCA) | yjfF → | predicted sugar transporter subunit |
| RA | 2,921,568 | +T | coding (455/1197 nt) | yjgN → | conserved inner membrane protein |
| RA | 2,973,690 | A→T | K29I (AAA→ATA) | nadR → | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | minE | 252010 | 252032 | 23 | 8 [6] | [7] 8 | malZ | maltodextrin glucosidase |
| * | * | ÷ | minE | 1616734 | 1616757 | 24 | 10 [6] | [5] 9 | eutE | predicted aldehyde dehydrogenase, ethanolamine utilization protein |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | minE | = 203208 | NA (NA) | 6 (0.110) | 5/112 | 2.9 | NA | noncoding (613/1542 nt) | rrsH | 16S ribosomal RNA |
| ? | minE | = 203220 | NA (NA) | noncoding (625/1542 nt) | rrsH | 16S ribosomal RNA | |||||
| * | ? | minE | = 204740 | NA (NA) | 9 (0.170) | 6/104 | 2.4 | NA | noncoding (157/2904 nt) | rrlH | 23S ribosomal RNA |
| ? | minE | = 204751 | NA (NA) | noncoding (168/2904 nt) | rrlH | 23S ribosomal RNA | |||||
| * | ? | minE | 207103 = | NA (NA) | 8 (0.140) | 5/114 | 2.9 | NA | noncoding (2520/2904 nt) | rrlH | 23S ribosomal RNA |
| ? | minE | 207128 = | NA (NA) | noncoding (2545/2904 nt) | rrlH | 23S ribosomal RNA | |||||
| * | ? | minE | = 1611756 | 10 (0.170) | 25 (0.430) +A |
9/118 | 2.1 | 43.1% | coding (1397/1404 nt) | eutA | reactivating factor for ethanolamine ammonia lyase |
| ? | minE | 2083040 = | 57 (0.950) | coding (66/663 nt) | yqjA | conserved inner membrane protein | |||||