Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,892,177 |
G→A |
47.6% |
D278N (GAC→AAC) |
ytfQ → |
predicted sugar transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,892,177 | 0 | G | A | 47.6%
| 8.4
/ 87.3
| 63 | D278N (GAC→AAC) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/16); new base A (21/9); total (38/25) |
| Fisher's exact test for biased strand distribution p-value = 1.98e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC > minE/2892111‑2892242
|
gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGACGAATATGGCAGGTCCCGCCTTCAAcgc > 1:253698/1‑71 (MQ=255)
gatgatGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCAAATATGGCAGGTCCCGCCTTCGACgcgc < 1:1458203/71‑1 (MQ=255)
gatggatgGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTg > 1:2263692/1‑70 (MQ=255)
tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:3170661/71‑1 (MQ=255)
tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2950030/71‑1 (MQ=255)
tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2722233/71‑1 (MQ=255)
tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag < 1:2312324/71‑1 (MQ=255)
gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga > 1:3109195/1‑70 (MQ=255)
gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga > 1:1016659/1‑70 (MQ=255)
gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGTTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1062231/70‑1 (MQ=255)
gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:3466392/70‑1 (MQ=255)
gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1036881/70‑1 (MQ=255)
gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa < 1:1723644/70‑1 (MQ=255)
gaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACa > 1:383313/1‑70 (MQ=255)
agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaa > 1:3735498/1‑70 (MQ=255)
agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa > 1:3716202/1‑70 (MQ=255)
gcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaa < 1:3578902/71‑1 (MQ=255)
gaaCCCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa > 1:3252216/1‑68 (MQ=255)
aCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGAc > 1:1028507/1‑71 (MQ=255)
cGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACg > 1:900112/1‑71 (MQ=255)
cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:487186/1‑71 (MQ=255)
cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:371540/1‑71 (MQ=255)
aaGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCa > 1:2622618/2‑71 (MQ=255)
aGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc < 1:1465471/71‑1 (MQ=255)
gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:1022778/1‑70 (MQ=255)
gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2944146/1‑70 (MQ=255)
gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2757459/1‑70 (MQ=255)
gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc > 1:2419946/1‑70 (MQ=255)
tgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATAc < 1:1588034/55‑1 (MQ=255)
ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGATAAATACAAAAAAGACGGCACCAt < 1:1927494/70‑1 (MQ=255)
gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGc > 1:2199687/1‑70 (MQ=255)
gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAAt > 1:3733289/1‑49 (MQ=255)
gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:114306/1‑71 (MQ=255)
gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:2829525/1‑71 (MQ=255)
aCTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt > 1:2616260/1‑70 (MQ=255)
cTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc > 1:155899/1‑68 (MQ=255)
aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa > 1:1359732/1‑51 (MQ=255)
aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa > 1:143461/1‑51 (MQ=255)
aCGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa < 1:602871/69‑1 (MQ=255)
gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaaaaa > 1:1591633/1‑49 (MQ=255)
gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:2686568/1‑71 (MQ=255)
gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:457196/1‑71 (MQ=255)
gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:2507619/1‑71 (MQ=255)
gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc > 1:3516704/1‑71 (MQ=255)
ccGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCt < 1:2175328/71‑1 (MQ=255)
gAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa > 1:16642/1‑71 (MQ=255)
tatGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt < 1:169628/71‑1 (MQ=255)
tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt < 1:962460/70‑1 (MQ=255)
tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa < 1:2248280/71‑1 (MQ=255)
gCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTaa < 1:3676005/70‑1 (MQ=255)
ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc > 1:1770742/1‑69 (MQ=255)
ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa > 1:2413012/1‑70 (MQ=255)
gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa < 1:2890479/71‑1 (MQ=255)
gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa < 1:2326991/71‑1 (MQ=255)
cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGAc > 1:1794505/1‑59 (MQ=255)
cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg > 1:2261450/1‑60 (MQ=255)
cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg > 1:1722575/1‑60 (MQ=255)
ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa > 1:3190382/1‑52 (MQ=255)
ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa > 1:435538/1‑52 (MQ=255)
ccGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc < 1:2360818/70‑1 (MQ=255)
gCCTTCGACGCGCTGGAGAAAAACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATacac < 1:1675265/71‑1 (MQ=255)
ccTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAcc < 1:2498058/71‑1 (MQ=255)
ttCGACGCGCTGGAGAAATACAAAAAAGACGGCAc < 1:558214/35‑1 (MQ=255)
|
GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC > minE/2892111‑2892242
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
