Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F21 I0 R1
|
148 |
22.8 |
1772010 |
97.0% |
1718849 |
61.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,632,877 |
A→G |
37.5% |
Y294C (TAC→TGC) |
nadR → |
bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,632,877 | 0 | A | G | 37.5%
| 15.9
/ 22.5
| 24 | Y294C (TAC→TGC) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (8/7); new base G (4/5); total (12/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACT > W3110S.gb/4632809‑4632941
|
ccTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATAc < 1:1104823/70‑1 (MQ=255)
atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCg > 1:361957/1‑71 (MQ=255)
atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACAt > 1:775913/1‑61 (MQ=255)
atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGc < 1:1369230/70‑1 (MQ=255)
cATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAatat < 1:1213922/71‑1 (MQ=255)
gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGcc > 1:625207/1‑70 (MQ=255)
cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCa < 1:667072/70‑1 (MQ=255)
cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCa < 1:1667893/70‑1 (MQ=255)
tATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaa < 1:558867/71‑1 (MQ=255)
ttCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGaaa > 1:557982/1‑58 (MQ=255)
cTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtt > 1:1434476/1‑45 (MQ=255)
tGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGa < 1:89570/53‑1 (MQ=255)
tGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGaaa > 1:1631581/1‑55 (MQ=255)
gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTgg > 1:579079/1‑70 (MQ=255)
aCTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGc < 1:382298/70‑1 (MQ=255)
gATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa < 1:966344/58‑1 (MQ=255)
tAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaaa > 1:1633356/1‑58 (MQ=255)
tAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaaa > 1:956/1‑58 (MQ=255)
aaaaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTgg > 1:496074/1‑61 (MQ=255)
gcgcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCACTTTATTGGCAtat < 1:1083208/60‑3 (MQ=25)
gcgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGc > 1:1458178/1‑56 (MQ=255)
gCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGt > 1:143175/1‑70 (MQ=255)
gCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTc < 1:716406/71‑1 (MQ=255)
cTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACt < 1:895410/71‑1 (MQ=255)
|
CCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACT > W3110S.gb/4632809‑4632941
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
