Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	385,180	G→T	23.2%	V130V (GTG→GTT)	cstA →	carbon starvation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	385,180	0	G	T	23.2%	124.2 / 22.8	56	V130V (GTG→GTT)	cstA	carbon starvation protein
Reads supporting (aligned to +/- strand): ref base G (20/23); new base T (3/10); total (23/33)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGA  >  minE/385112‑385248

gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg                                                                      >  1:1625793/1‑69 (MQ=255)

   gcgcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAg                                                                   <  1:2642605/69‑1 (MQ=255)

    cgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGac                                                                <  1:4336456/71‑2 (MQ=255)

    cgcAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGac                                                                <  1:3892571/71‑2 (MQ=255)

     gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                                                               >  1:5187398/1‑71 (MQ=255)

      cagaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActt                                                              <  1:4055455/68‑1 (MQ=255)

      cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                              <  1:1112480/71‑1 (MQ=255)

        gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                            <  1:5612495/70‑3 (MQ=255)

        gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                            >  1:3683789/2‑69 (MQ=255)

        gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                            <  1:1917284/70‑3 (MQ=255)

          tGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATg                                                          <  1:4973445/71‑1 (MQ=255)

          tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTAGCCGGTGCGGTGCAGGATTTCATg                                                          >  1:5038970/1‑71 (MQ=255)

               tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt                                                     >  1:268300/1‑71 (MQ=255)

               tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt                                                     >  1:2429487/1‑71 (MQ=255)

                 ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                                   <  1:3102020/71‑1 (MQ=255)

                  cTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtt                                                  >  1:4242997/1‑71 (MQ=255)

                     ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                              <  1:808785/56‑1 (MQ=255)

                     ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                               >  1:4970713/1‑71 (MQ=255)

                       ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                                            >  1:3114377/1‑56 (MQ=255)

                       ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                                            >  1:890358/1‑56 (MQ=255)

                         ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                           <  1:416204/71‑1 (MQ=255)

                         ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                           <  1:3221073/71‑1 (MQ=255)

                          gatgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                           <  1:356398/70‑1 (MQ=255)

                             gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAcgc                                       >  1:4146158/1‑71 (MQ=255)

                              atCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                              <  1:3860205/47‑1 (MQ=255)

                              atCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                              <  1:3721332/47‑1 (MQ=255)

                                 tGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGTTGCAGGAtt                                                               <  1:3479728/43‑1 (MQ=255)

                                   gctgctGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                  <  1:1542498/70‑1 (MQ=255)

                                   gctgctGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                  <  1:3090677/70‑1 (MQ=255)

                                   gctgctGGCGGGGGGCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                  <  1:500199/70‑1 (MQ=255)

                                   gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                    <  1:4305117/68‑1 (MQ=255)

                                   gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                  <  1:2458152/70‑1 (MQ=255)

                                   gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                  <  1:4418362/70‑1 (MQ=255)

                                   gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                  <  1:3200192/70‑1 (MQ=255)

                                    ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCtgttt                                                 <  1:1922410/54‑1 (MQ=255)

                                         cGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                           >  1:5490728/1‑71 (MQ=255)

                                          gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcg                          >  1:3046235/1‑71 (MQ=255)

                                             ggggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                            <  1:5310234/66‑1 (MQ=255)

                                              gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                       <  1:1595512/70‑1 (MQ=255)

                                               ggtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctcgc                     >  1:3698662/1‑71 (MQ=255)

                                                gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                    <  1:1056214/71‑1 (MQ=255)

                                                   gtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTa                                          <  1:1259021/46‑1 (MQ=255)

                                                     tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                >  1:1874767/1‑70 (MQ=255)

                                                     tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                >  1:4414246/1‑70 (MQ=255)

                                                     tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                >  1:4562062/1‑70 (MQ=255)

                                                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTa                                          >  1:5014995/1‑43 (MQ=255)

                                                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                >  1:2785190/1‑69 (MQ=255)

                                                      ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg               <  1:614695/70‑1 (MQ=255)

                                                       tcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTc                                            <  1:365446/40‑1 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgtt                                              >  1:372742/1‑36 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                             >  1:2593068/1‑37 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                             >  1:400031/1‑37 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                             >  1:2460449/1‑37 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTc                                            <  1:5469044/38‑1 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGc            <  1:2879691/70‑1 (MQ=255)

                                                         gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCt           >  1:2012251/1‑71 (MQ=255)

                                                          ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg          <  1:5132589/71‑1 (MQ=255)

                                                            ggtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGt        >  1:3763399/1‑71 (MQ=255)

                                                             gtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTc       <  1:1909713/71‑1 (MQ=255)

                                                              tgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACgg                             <  1:2536175/48‑1 (MQ=255)

                                                                 ggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag   <  1:1055205/71‑1 (MQ=255)

                                                                  gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGCTCGCTCGCTGGGTGAGCTGGTCAaaga  <  1:1892611/71‑1 (MQ=255)

GCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGA  >  minE/385112‑385248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A