Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,315 |
G→T |
39.9% |
L11L (CTG→CTT) |
narH → |
nitrate reductase 1, beta (Fe‑S) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,315 | 0 | G | T | 39.9%
| 146.5
/ 125.6
| 135 | L11L (CTG→CTT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (41/40); new base T (29/25); total (70/65) |
| Fisher's exact test for biased strand distribution p-value = 8.61e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 136 total aligned reads displayed.
GATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGA > minE/835245‑835383
|
gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:236991/71‑1 (MQ=255)
aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa < 1:2242913/71‑1 (MQ=255)
gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:1585040/71‑1 (MQ=255)
gAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc < 1:5349937/71‑1 (MQ=255)
aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:3902747/71‑1 (MQ=255)
ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4381332/71‑1 (MQ=255)
aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4613436/1‑71 (MQ=255)
aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4002944/1‑71 (MQ=255)
aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGGGCTGAATCTCGATaa < 1:5613257/70‑1 (MQ=255)
aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1670486/1‑70 (MQ=255)
tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3005167/2‑71 (MQ=255)
tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:2954352/2‑71 (MQ=255)
ccAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:3122629/70‑1 (MQ=255)
cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:556670/1‑71 (MQ=255)
cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc > 1:2118422/1‑71 (MQ=255)
aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:1446184/1‑71 (MQ=255)
aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2580379/1‑63 (MQ=255)
aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2366678/1‑63 (MQ=255)
aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACaaat > 1:2155698/1‑63 (MQ=255)
gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4453539/59‑1 (MQ=255)
aCAGGAGAGCG‑TAAAATGAGAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:2227744/1‑71 (MQ=255)
aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:1495309/1‑71 (MQ=255)
gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcac < 1:3211809/71‑1 (MQ=255)
agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:3287573/1‑48 (MQ=255)
agCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACAcc < 1:104920/71‑1 (MQ=255)
cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:3072600/1‑40 (MQ=255)
cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa > 1:2775739/1‑40 (MQ=255)
aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:892720/69‑1 (MQ=255)
aaaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGt < 1:2601292/69‑1 (MQ=255)
tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt < 1:829840/71‑1 (MQ=255)
aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:458403/2‑61 (MQ=255)
aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:1924013/2‑61 (MQ=255)
aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGc > 1:124944/2‑61 (MQ=255)
aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:4462691/70‑1 (MQ=255)
aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:3431545/70‑1 (MQ=255)
aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTAt > 1:3815755/1‑53 (MQ=255)
aaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc > 1:2363345/1‑71 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3377056/1‑37 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3621258/1‑37 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGa < 1:5326309/36‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:4103550/1‑43 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:485090/1‑43 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2027004/45‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:5139248/45‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:4720835/45‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:4634673/48‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:3425474/48‑1 (MQ=255)
aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4398764/70‑1 (MQ=255)
aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:4297083/1‑37 (MQ=38)
aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1661258/1‑37 (MQ=38)
aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTaac > 1:1654626/1‑37 (MQ=38)
aaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTcg > 1:2178191/1‑70 (MQ=255)
aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:2977072/49‑2 (MQ=255)
aaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATgt < 1:1707439/49‑2 (MQ=255)
aTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGt > 1:2942982/1‑71 (MQ=255)
tGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:2007373/67‑1 (MQ=255)
tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:3807655/46‑1 (MQ=255)
tGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATg < 1:1345615/46‑1 (MQ=255)
aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255)
aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:2988737/1‑71 (MQ=255)
aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5576613/40‑3 (MQ=255)
aaaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGaca < 1:5583323/40‑3 (MQ=255)
aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:3033300/1‑71 (MQ=255)
aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:844935/1‑40 (MQ=255)
aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:1941724/1‑40 (MQ=255)
aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:4641487/1‑40 (MQ=255)
aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg > 1:31596/1‑40 (MQ=255)
aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAcc < 1:5463800/70‑1 (MQ=255)
aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:4538003/1‑44 (MQ=255)
aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:2679041/1‑44 (MQ=255)
aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAg > 1:5531578/1‑64 (MQ=255)
ttCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGt < 1:1202460/71‑1 (MQ=255)
tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGtg < 1:2521365/63‑2 (MQ=255)
ccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTg > 1:4929890/2‑69 (MQ=255)
gTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:39445/71‑1 (MQ=255)
gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:3824596/70‑1 (MQ=255)
gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:2735648/70‑1 (MQ=255)
ttCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1244187/56‑1 (MQ=255)
ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaa < 1:2080557/70‑1 (MQ=255)
tCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc < 1:2813408/45‑1 (MQ=255)
cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCca > 1:5225096/1‑46 (MQ=255)
cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc > 1:2548851/1‑71 (MQ=255)
acaAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACg > 1:3183088/1‑71 (MQ=255)
caAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:807893/52‑1 (MQ=255)
aaGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaaa > 1:5685662/1‑66 (MQ=255)
aGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCt > 1:503056/1‑71 (MQ=255)
tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:5621635/71‑1 (MQ=255)
ggCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAc < 1:4918992/71‑1 (MQ=255)
aTGGTGCTGAATCTCTATAAGTGCATCGGCTGCCACAcc < 1:1000773/39‑1 (MQ=255)
tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt < 1:1798066/42‑1 (MQ=255)
tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc < 1:2512733/43‑1 (MQ=255)
tGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTc < 1:836334/43‑1 (MQ=255)
tGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:4865397/43‑1 (MQ=255)
gTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:4608998/1‑62 (MQ=255)
gTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCg < 1:696788/71‑1 (MQ=255)
gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:921225/1‑50 (MQ=255)
gCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTACCTGTaa < 1:757831/51‑1 (MQ=255)
gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255)
cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4223772/1‑69 (MQ=255)
cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:4804319/70‑1 (MQ=255)
|
GATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGA > minE/835245‑835383
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
