Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	836,047	G→A	30.4%	Q255Q (CAG→CAA)	narH →	nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	836,047	0	G	A	30.4%	92.2 / 47.9	69	Q255Q (CAG→CAA)	narH	nitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand): ref base G (17/31); new base A (10/11); total (27/42)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGAC  >  minE/835981‑836116

cTTCAACTGGAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAAcc                                                                     <  1:3325358/69‑1 (MQ=255)

   cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAg                                                                       >  1:688396/1‑64 (MQ=255)

   cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAg                                                                       >  1:1748580/1‑64 (MQ=255)

   cAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGAGGGTCAg                                                                       >  1:5459720/1‑64 (MQ=255)

    aaCTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg                                                                <  1:1274678/70‑1 (MQ=255)

        ggAAAAGCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGc                                                           >  1:1181434/1‑71 (MQ=255)

         gAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc                                                           <  1:2373275/70‑1 (MQ=255)

              gCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGaa                                                     <  1:3124529/71‑1 (MQ=255)

              gCGGCAAGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGaa                                                     <  1:2603101/71‑1 (MQ=255)

                 gTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCg                                                                <  1:3512480/57‑1 (MQ=255)

                  tAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGTGTCAGCCGACCGTGTGCTCAGAAACCt                                                 <  1:3052700/71‑1 (MQ=255)

                    aGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCtgt                                               >  1:1209816/1‑71 (MQ=255)

                    aGTCAGAAAAATGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCtgc                                               >  1:1711302/1‑70 (MQ=255)

                      tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc                                                           <  1:4415156/57‑1 (MQ=255)

                      tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc                                                           <  1:3871235/57‑1 (MQ=255)

                      tCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGc                                                           <  1:3714398/57‑1 (MQ=255)

                          agaAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcggt                                         <  1:4389993/71‑1 (MQ=255)

                          agaAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTgtcgg                                          <  1:508103/70‑1 (MQ=255)

                            aaatGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCgtgggtc                                        >  1:777092/4‑70 (MQ=255)

                             aatGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGaa                                                     <  1:1557606/54‑1 (MQ=255)

                             aatGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGaa                                                     <  1:4917612/54‑1 (MQ=255)

                             aatGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCgtgggtcgc                                      <  1:1656730/69‑2 (MQ=255)

                             aGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGGCGGTCGt                                      <  1:523969/71‑1 (MQ=255)

                              atGCATCTTCTGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCgtgggtcgca                                     >  1:3327909/2‑69 (MQ=255)

                               tGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTAt                                    <  1:401398/71‑1 (MQ=255)

                                gCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAAcc                                                  <  1:1254123/56‑1 (MQ=255)

                                  aTCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATcct                                 <  1:5139350/71‑2 (MQ=255)

                                  aTCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCg                                 <  1:4443711/71‑1 (MQ=255)

                                  aTCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCg                                 <  1:1523005/71‑1 (MQ=255)

                                  aTCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAAACTGTGTCGGTCGTATCCg                                 <  1:566046/71‑1 (MQ=255)

                                   tcttctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGtgtg                                                            <  1:2499695/43‑1 (MQ=255)

                                    cttctGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGa                                                      <  1:4473373/48‑1 (MQ=255)

                                     ttctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTa                              >  1:5082527/1‑71 (MQ=255)

                                      tctGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTAt                             >  1:3191624/1‑71 (MQ=255)

                                      tctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTAt                             >  1:58117/1‑71 (MQ=255)

                                       ctGTTACCCACGAATTGAGTCCGGTCAACCGACCGTGTGCTCAGAAACCTGCgtgggtcgca                                     <  1:2475869/62‑3 (MQ=255)

                                       ctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGtt                               <  1:675981/68‑1 (MQ=255)

                                       ctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGtt                               <  1:5263314/68‑1 (MQ=255)

                                       ctGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTa                              <  1:5161340/69‑1 (MQ=255)

                                          cTATCCGCGTATTTAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCt                           <  1:4448824/69‑1 (MQ=255)

                                             tCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCtt                          <  1:2319512/67‑1 (MQ=255)

                                             tCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCtt                          <  1:2269130/67‑1 (MQ=255)

                                               cgcgTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGt                     <  1:1879847/70‑1 (MQ=255)

                                                gcgTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGc                   >  1:2062906/1‑71 (MQ=255)

                                                 cgTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCt                  >  1:208666/1‑71 (MQ=255)

                                                    aTTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCt                  >  1:2992793/1‑68 (MQ=255)

                                                    aTTGAAGCGGGTCAGACGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgtt               >  1:2292953/1‑71 (MQ=255)

                                                     ttGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              >  1:5677282/1‑71 (MQ=255)

                                                      tGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              >  1:866778/1‑70 (MQ=255)

                                                      tGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              <  1:2321273/70‑1 (MQ=255)

                                                      tGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              >  1:4447281/1‑70 (MQ=255)

                                                      tGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              >  1:3838078/1‑70 (MQ=255)

                                                      tGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCtgttg              >  1:3607389/1‑70 (MQ=255)

                                                       gAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGAGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTa            >  1:269440/1‑71 (MQ=255)

                                                        aaGCGTGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTa            <  1:3936445/70‑1 (MQ=255)

                                                         aGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACg          <  1:4309061/71‑1 (MQ=255)

                                                          tccggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCt                  <  1:4354187/59‑1 (MQ=255)

                                                          tccggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCt                  <  1:1297308/59‑1 (MQ=255)

                                                          tccggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCTGCTTTACGa         >  1:276075/4‑71 (MQ=255)

                                                          gCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGa         >  1:4667558/1‑71 (MQ=255)

                                                            cggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCtgc                >  1:3915422/2‑61 (MQ=255)

                                                            cggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCtgc                >  1:5670470/2‑61 (MQ=255)

                                                            cggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCtgc                >  1:1297300/2‑61 (MQ=255)

                                                            cggTCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCTGCTTTACGac        <  1:812112/69‑2 (MQ=255)

                                                              gTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTAc           <  1:2662269/65‑1 (MQ=255)

                                                               tCAACCGACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCGGTATCTGGGCGTGCTGCTTTACGACGCCg    <  1:5551407/71‑1 (MQ=255)

                                                               tCAACCGACCGTGTGCTCAGAAACCTGCATGGGTCGCATCCGGTATCTGGGCGTGCTGCTTTACGACGcc     >  1:2781159/1‑70 (MQ=255)

                                                                cAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGa   >  1:1801949/1‑71 (MQ=255)

                                                                 aGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGc                       <  1:3612705/50‑1 (MQ=255)

                                                                 aGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGc                       <  1:2213539/50‑1 (MQ=255)

                                                                 aGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGAc  >  1:4856313/1‑71 (MQ=255)

                                                                 aGCAGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGAc  >  1:4834711/1‑71 (MQ=255)

                                                                  gCCGACCGTGTGATCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGcc     <  1:1669589/67‑1 (MQ=255)

CTTCAACTGGAAGAGCGGTAAGTCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCAGAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGAC  >  minE/835981‑836116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A