Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,014,698 |
A→G |
19.3% |
S457S (AGT→AGC) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,014,698 | 0 | A | G | 19.3%
| 167.5
/ 33.7
| 83 | S457S (AGT→AGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (30/37); new base G (9/7); total (39/44) |
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAG > minE/1014630‑1014765
|
aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCt < 1:530231/71‑1 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg > 1:4858543/1‑71 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:4932291/71‑1 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5149872/71‑1 (MQ=255)
tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcgc > 1:3348219/1‑71 (MQ=255)
tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:118167/1‑70 (MQ=255)
tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg < 1:2412482/70‑1 (MQ=255)
cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc < 1:3500963/69‑1 (MQ=255)
cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa < 1:1257073/70‑1 (MQ=255)
cATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1866316/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:785037/1‑71 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3708461/1‑44 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3604764/1‑44 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5604358/48‑1 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5680458/48‑1 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa > 1:72032/1‑61 (MQ=255)
aCCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACAt > 1:2767763/1‑70 (MQ=255)
gctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa > 1:3249287/1‑71 (MQ=255)
gctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGag > 1:472056/1‑70 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:349343/57‑1 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGa < 1:3328560/69‑1 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCAACATAAGa < 1:2458362/69‑1 (MQ=255)
ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa > 1:5578970/1‑66 (MQ=255)
ggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc > 1:538187/1‑50 (MQ=255)
gactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc > 1:2740101/3‑70 (MQ=255)
ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccat > 1:919753/1‑71 (MQ=255)
ctgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccat < 1:635382/71‑1 (MQ=255)
tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCAcgt < 1:5649444/60‑3 (MQ=255)
tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccata > 1:33357/1‑70 (MQ=255)
gCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:4892227/1‑71 (MQ=255)
gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca < 1:3370554/65‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:1075610/44‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:5237625/44‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:4838559/44‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:4813594/44‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAg < 1:469002/44‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtcc > 1:2943055/1‑71 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCATGCAg < 1:4093900/44‑1 (MQ=255)
taccTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:2604965/3‑70 (MQ=255)
tGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:2437893/1‑71 (MQ=255)
gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg > 1:153873/1‑70 (MQ=255)
gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgt < 1:4345819/67‑1 (MQ=255)
accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3405713/2‑69 (MQ=255)
accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:5384115/2‑69 (MQ=255)
accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg > 1:3270859/2‑69 (MQ=255)
accTTTCGCCAGCATGATCATGCCGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct > 1:5142767/2‑69 (MQ=255)
cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggcg > 1:2273606/1‑71 (MQ=255)
tttCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtcca < 1:4624087/59‑1 (MQ=255)
ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:3373890/1‑71 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:1007613/1‑47 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:2347324/1‑47 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt < 1:1482870/70‑1 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:4617686/1‑71 (MQ=255)
gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCggtggt < 1:208187/71‑1 (MQ=255)
gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGtg < 1:2335407/69‑2 (MQ=255)
gCCAGCATGATCATACTTCCGCCCTGCGCTAGCAGTTGATCGACATAAGaa > 1:179943/1‑51 (MQ=255)
ccAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGtt < 1:5185377/71‑1 (MQ=255)
ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatc < 1:3114174/52‑1 (MQ=255)
ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccg < 1:5585939/58‑1 (MQ=255)
ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:3198675/70‑1 (MQ=255)
aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg > 1:1936462/1‑71 (MQ=255)
aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg < 1:3163240/71‑1 (MQ=255)
cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:5507567/66‑1 (MQ=255)
cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2295277/1‑71 (MQ=255)
tgttCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCa < 1:3217070/68‑1 (MQ=255)
tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:469015/40‑1 (MQ=255)
tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:2032453/40‑1 (MQ=255)
tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATa < 1:4895398/40‑1 (MQ=255)
tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1444583/1‑66 (MQ=255)
tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:4603298/70‑1 (MQ=255)
gATCATACTTCCGCCCTGTGCTTGCAGTTGATCGACATAAGaa < 1:1828306/43‑1 (MQ=255)
gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:2871881/64‑1 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:3933305/1‑49 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc > 1:2968097/1‑49 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:3905659/43‑1 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:430467/43‑1 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:4334084/43‑1 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:520481/1‑68 (MQ=255)
tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:5372804/39‑1 (MQ=255)
tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1640576/39‑1 (MQ=255)
tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg < 1:1201293/39‑1 (MQ=255)
tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg > 1:4616898/1‑60 (MQ=255)
aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAagag > 1:5288592/1‑70 (MQ=255)
aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:276670/1‑35 (MQ=255)
aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:917123/1‑35 (MQ=255)
|
AAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAG > minE/1014630‑1014765
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
