Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,191,560 |
T→C |
69.8% |
*116W (TGA→TGG) |
rnd ← |
ribonuclease D |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,191,560 | 0 | T | C | 69.8%
| 101.0
/ 54.2
| 76 | *116W (TGA→TGG) | rnd | ribonuclease D |
| Reads supporting (aligned to +/- strand): ref base T (13/10); new base C (29/24); total (42/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 |
AGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCA > minE/1191493‑1191629
|
agTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCAt < 1:1695743/71‑1 (MQ=255)
agTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCAt < 1:1286880/71‑1 (MQ=255)
ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACa < 1:1116261/71‑1 (MQ=255)
ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACa > 1:3435791/1‑71 (MQ=255)
gtgtGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt < 1:3979065/71‑1 (MQ=255)
gTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt < 1:2902803/71‑1 (MQ=255)
tGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt < 1:5683018/70‑1 (MQ=255)
gcgcGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCg > 1:5558734/1‑71 (MQ=255)
cgcgATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCgg < 1:694846/71‑1 (MQ=255)
cgcgATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCgg > 1:3338439/1‑71 (MQ=255)
gATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCg > 1:5660801/1‑71 (MQ=255)
gATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCg > 1:5055130/1‑71 (MQ=255)
aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt > 1:2609941/1‑71 (MQ=255)
aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt > 1:2189164/1‑71 (MQ=255)
aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt > 1:4860554/1‑71 (MQ=255)
ttCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTc > 1:5271823/1‑71 (MQ=255)
tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTcc > 1:3696132/1‑71 (MQ=255)
tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGa > 1:327485/1‑59 (MQ=255)
tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGa > 1:305567/1‑59 (MQ=255)
tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc > 1:3557863/1‑71 (MQ=255)
tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc > 1:1595948/1‑71 (MQ=255)
cACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCg > 1:1966608/1‑71 (MQ=255)
aCTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCg > 1:885320/1‑70 (MQ=255)
ctTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg > 1:3705913/1‑70 (MQ=255)
gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCg > 1:1779109/1‑60 (MQ=255)
gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa < 1:1913818/68‑1 (MQ=255)
gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa < 1:3630306/68‑1 (MQ=255)
gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa < 1:1248167/68‑1 (MQ=255)
tCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgc < 1:4957590/71‑1 (MQ=255)
ccAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg < 1:3247608/65‑1 (MQ=255)
aGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGgcagc < 1:385931/71‑1 (MQ=255)
cGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgcagc > 1:2093728/1‑69 (MQ=255)
gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAg < 1:5166510/71‑1 (MQ=255)
gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg > 1:5430549/1‑71 (MQ=255)
gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg > 1:2734450/1‑71 (MQ=255)
aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCa > 1:3141957/1‑38 (MQ=255)
aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCa > 1:4276948/1‑56 (MQ=255)
aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGa < 1:2336051/70‑1 (MQ=255)
aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAt > 1:3874343/1‑71 (MQ=255)
ccGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg < 1:5148212/71‑1 (MQ=255)
aaTACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg < 1:2196711/50‑1 (MQ=255)
tACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc < 1:3846381/71‑1 (MQ=255)
ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtg < 1:5388374/67‑1 (MQ=255)
ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaa > 1:4890908/1‑71 (MQ=255)
ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca < 1:5055779/70‑1 (MQ=255)
tctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtg < 1:4542072/66‑1 (MQ=255)
ttCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc < 1:5054368/61‑1 (MQ=255)
tCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa < 1:2020294/43‑1 (MQ=255)
tCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa < 1:1651101/43‑1 (MQ=255)
ccaccaTGGAAGCGAAACCCCATGACATCGGGCGTTCGCAg > 1:124873/1‑41 (MQ=38)
caccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGTGTGTCAATCAAg < 1:5235365/70‑1 (MQ=255)
accaTGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAggg > 1:1039116/1‑71 (MQ=255)
ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc < 1:4147045/61‑1 (MQ=255)
ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGt < 1:2197162/71‑1 (MQ=255)
ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGt < 1:372372/71‑1 (MQ=255)
caTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGtt < 1:5414120/71‑1 (MQ=255)
aTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTtg > 1:1244167/1‑71 (MQ=255)
tGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc < 1:4956068/53‑1 (MQ=255)
tGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc < 1:4962434/53‑1 (MQ=255)
tGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg < 1:3096740/47‑1 (MQ=255)
ggAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc < 1:1310722/52‑1 (MQ=255)
gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa > 1:445553/1‑35 (MQ=255)
gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa > 1:4091739/1‑35 (MQ=255)
gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa > 1:1961488/1‑35 (MQ=255)
gAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTgg > 1:5073327/1‑71 (MQ=255)
gAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTgg > 1:5068180/1‑71 (MQ=255)
aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAtt > 1:1495634/1‑46 (MQ=255)
aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg > 1:374586/1‑48 (MQ=255)
aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg > 1:5247547/1‑48 (MQ=255)
aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg > 1:3264401/1‑48 (MQ=255)
aGCGAAACCTCAAGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg > 1:48360/1‑48 (MQ=255)
aGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGt > 1:4701558/1‑70 (MQ=255)
gAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg > 1:3840118/1‑40 (MQ=255)
gAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg > 1:2360993/1‑40 (MQ=255)
aCCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGc > 1:1830921/1‑71 (MQ=255)
cTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCa < 1:2128028/71‑1 (MQ=255)
|
AGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCA > minE/1191493‑1191629
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
