Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,314,255 T→C 78.3% H170H (CAT→CAC)  hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,314,2550TC78.3% 258.4 / 64.2 134H170H (CAT→CAC) hisBfused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (12/17);  new base C (47/57);  total (60/74)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 134 total aligned reads displayed.

CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC  >  minE/1314186‑1314321
                                                                     |                                                                  
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg                                                                   <  1:1035984/71‑1 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg                                                                   >  1:5284348/1‑71 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg                                                                   >  1:5290793/1‑71 (MQ=255)
   cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt                                                                  <  1:3019115/69‑1 (MQ=255)
     aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTg                                                              >  1:5393828/1‑71 (MQ=255)
      ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc                                                             <  1:5486157/71‑1 (MQ=255)
        gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                                                           <  1:3418228/71‑1 (MQ=255)
        gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                                                           <  1:1436222/71‑1 (MQ=255)
         cgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                                                          <  1:4921825/71‑1 (MQ=255)
          gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa                                                         <  1:697410/71‑1 (MQ=255)
          gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa                                                         <  1:161905/71‑1 (MQ=255)
          gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                                                          <  1:4463972/70‑1 (MQ=255)
           aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                                                         >  1:4019501/1‑70 (MQ=255)
           aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACCCTCACGTAGTGCGTaa                                                         >  1:4887259/1‑70 (MQ=255)
               ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTTACCGTTACGCTCACGTAGTGCGTAATACCa                                                    <  1:1049798/71‑1 (MQ=255)
                   aaCTGGCCAATGATTGGCGAGCAACTCACGAGACGTGACCGTTACGCTCATGTAGTGCGTaa                                                         >  1:1794010/1‑62 (MQ=255)
                   aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                                                  <  1:5418528/69‑1 (MQ=255)
                   aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                                                <  1:5573430/71‑1 (MQ=255)
                   aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                                                <  1:5454323/71‑1 (MQ=255)
                    aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                                                  <  1:3783031/68‑1 (MQ=255)
                        gCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAc                                             <  1:4331655/69‑1 (MQ=255)
                          cAATGATTGGCGAGCAACTCACCAGACGTTACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg                                         <  1:2425948/71‑1 (MQ=255)
                          cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg                                         <  1:452643/71‑1 (MQ=255)
                           aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                                                           <  1:3676277/52‑1 (MQ=255)
                           aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg                                            >  1:3845091/1‑67 (MQ=255)
                            aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt                                       >  1:5554369/1‑71 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa                                                         >  1:4960015/1‑52 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      <  1:3982568/71‑1 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      <  1:2719386/71‑1 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      >  1:2258133/1‑71 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      >  1:4154477/1‑71 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      >  1:4676729/1‑71 (MQ=255)
                             tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      <  1:580656/71‑1 (MQ=255)
                              gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      >  1:1780201/1‑70 (MQ=255)
                              gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                      >  1:1169088/1‑70 (MQ=255)
                              gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt                                       >  1:3577223/1‑69 (MQ=255)
                              gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt                                       >  1:3396886/1‑69 (MQ=255)
                               aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg                                     <  1:3616828/70‑1 (MQ=255)
                               aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg                                     <  1:5613962/70‑1 (MQ=255)
                               aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg                                     <  1:1016402/70‑1 (MQ=255)
                                ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaa                                                   >  1:1744816/1‑55 (MQ=255)
                                 tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAAGCAGATTGACg                                  >  1:522661/1‑71 (MQ=255)
                                   gCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGtt                                >  1:2442696/1‑71 (MQ=255)
                                    cGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc                               <  1:2299890/71‑1 (MQ=255)
                                     gAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                              <  1:647680/71‑1 (MQ=255)
                                       gCAACTCACCAGACGTGACCGTTACGCTCAAGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            >  1:5271006/1‑71 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGtt                                >  1:4729005/1‑64 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc                               >  1:636189/1‑65 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc                               >  1:2513810/1‑65 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc                               >  1:4582466/1‑65 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:1594189/68‑1 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:101533/68‑1 (MQ=255)
                                          aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:4472738/68‑1 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:5612649/67‑1 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:4182052/67‑1 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:1945575/67‑1 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg                        >  1:3233315/1‑71 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg                        >  1:4406199/1‑71 (MQ=255)
                                           cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg                        >  1:3240178/1‑71 (MQ=255)
                                            tCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg                       >  1:3614551/1‑71 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg                                     >  1:2138166/1‑56 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                              >  1:1065203/1‑63 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                              >  1:2544391/1‑63 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                      >  1:4318026/1‑71 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                      >  1:1401442/1‑71 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                      >  1:4563584/1‑71 (MQ=255)
                                             cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                      >  1:2172168/1‑71 (MQ=255)
                                             cACCAGACGTGACAGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                      >  1:2685996/1‑71 (MQ=255)
                                                cAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg                    >  1:2133591/1‑70 (MQ=255)
                                                 aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg                                         >  1:1425381/1‑48 (MQ=255)
                                                 aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa                  <  1:2535897/71‑1 (MQ=255)
                                                  gACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga                                              >  1:3219539/1‑42 (MQ=255)
                                                   aCGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                              <  1:1636999/57‑1 (MQ=255)
                                                    cGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg               <  1:4420331/71‑1 (MQ=255)
                                                     gTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga                                                <  1:1056390/37‑1 (MQ=255)
                                                      tGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg             <  1:3385040/71‑1 (MQ=255)
                                                      tGACCGTTACGCTCACGTAGTGAGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg             <  1:2456677/71‑1 (MQ=255)
                                                        aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa           <  1:4347094/71‑1 (MQ=255)
                                                        aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa            <  1:413611/70‑1 (MQ=255)
                                                          cGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         <  1:755584/71‑1 (MQ=255)
                                                          cGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         <  1:4539499/71‑1 (MQ=255)
                                                          cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         >  1:495521/1‑71 (MQ=255)
                                                          cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         <  1:4255181/71‑1 (MQ=255)
                                                          cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         <  1:1151664/71‑1 (MQ=255)
                                                          cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg         <  1:1551010/71‑1 (MQ=255)
                                                           gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:183651/51‑1 (MQ=255)
                                                           gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:3163709/51‑1 (MQ=255)
                                                           gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:1533976/51‑1 (MQ=255)
                                                            ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa           <  1:1202911/67‑1 (MQ=255)
                                                            ttACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg                            <  1:4106278/50‑1 (MQ=255)
                                                              aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg                             >  1:3697066/1‑47 (MQ=255)
                                                              aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt                         >  1:4201326/1‑51 (MQ=255)
                                                              aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt                         >  1:4765381/1‑51 (MQ=255)
                                                              aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt                         >  1:3174328/1‑51 (MQ=255)
                                                              aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg          >  1:5050649/1‑66 (MQ=255)
                                                               cGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg                             >  1:38727/1‑46 (MQ=255)
                                                               cGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg             <  1:4380693/62‑1 (MQ=255)
                                                                 cTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa           >  1:550617/1‑62 (MQ=255)
                                                                 cTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa           >  1:2711543/1‑62 (MQ=255)
                                                                  tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                              >  1:4030393/1‑42 (MQ=255)
                                                                     |                                                                  
CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC  >  minE/1314186‑1314321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A