Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,614,492 |
T→C |
50.9% |
intergenic (‑117/+100) |
eutH ← / ← eutG |
predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,614,492 | 0 | T | C | 50.9%
| 23.0
/ 77.4
| 59 | intergenic (‑117/+100) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base T (17/12); new base C (14/16); total (31/28) |
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCT > minE/1614422‑1614559
|
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3856953/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:1797315/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt < 1:5201586/71‑1 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:2797065/1‑71 (MQ=38)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:38098/1‑70 (MQ=11)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:236485/1‑70 (MQ=11)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:5672081/1‑70 (MQ=11)
ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:1479959/71‑1 (MQ=14)
ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:3840454/71‑1 (MQ=14)
ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:1914965/1‑71 (MQ=255)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:5187757/1‑64 (MQ=39)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:5126278/1‑69 (MQ=255)
aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACcgc > 1:2750132/1‑71 (MQ=14)
aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc < 1:502711/69‑1 (MQ=255)
cccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc < 1:4959017/71‑1 (MQ=255)
tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:5323426/1‑71 (MQ=255)
tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:3925711/1‑71 (MQ=255)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:3817910/71‑1 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:807848/71‑1 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:1671830/71‑1 (MQ=14)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:4556734/71‑1 (MQ=255)
aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg > 1:4747650/1‑70 (MQ=255)
cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:5055456/71‑1 (MQ=255)
cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgc < 1:2173726/70‑1 (MQ=255)
aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcgg < 1:3088174/71‑1 (MQ=255)
ccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:988845/71‑1 (MQ=11)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:1764484/1‑58 (MQ=255)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:26239/1‑58 (MQ=255)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGt > 1:1779074/1‑70 (MQ=255)
cGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg > 1:4811753/1‑70 (MQ=11)
gtcggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:4506289/62‑1 (MQ=11)
cggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCt > 1:3433141/2‑70 (MQ=14)
ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa > 1:2150067/1‑71 (MQ=255)
ggATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCtt < 1:705783/70‑1 (MQ=14)
gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTg < 1:2918214/47‑1 (MQ=255)
gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:969245/50‑1 (MQ=255)
gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:1463460/1‑71 (MQ=255)
gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:4773168/1‑71 (MQ=255)
aTAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc > 1:2344458/1‑71 (MQ=18)
tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATcc > 1:4978755/1‑71 (MQ=255)
aaaaCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCg > 1:1016883/4‑71 (MQ=18)
aaaaCGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCg > 1:5645550/4‑71 (MQ=18)
gACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc > 1:4877157/1‑70 (MQ=255)
cgcgACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGccc > 1:2658491/1‑70 (MQ=17)
cgGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTAc < 1:5262566/71‑1 (MQ=255)
gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACa > 1:2468543/3‑71 (MQ=18)
aaGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt < 1:3885686/71‑1 (MQ=255)
aaGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgt > 1:606767/1‑71 (MQ=21)
gCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGc < 1:3671484/71‑1 (MQ=21)
gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCa < 1:939301/70‑1 (MQ=255)
ttgCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa < 1:2801232/59‑1 (MQ=255)
gCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAAtt < 1:4596660/70‑2 (MQ=21)
gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATcc > 1:2122357/1‑71 (MQ=255)
aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg < 1:2698853/71‑1 (MQ=255)
tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:4818242/1‑70 (MQ=18)
tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCg > 1:1423884/1‑70 (MQ=18)
tCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAt > 1:762628/1‑48 (MQ=255)
tCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGt < 1:1751831/71‑1 (MQ=21)
cGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTa < 1:2153166/70‑1 (MQ=18)
cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1830344/70‑1 (MQ=255)
cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1247214/70‑1 (MQ=255)
cGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAg < 1:3673076/71‑1 (MQ=18)
gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc > 1:3780197/1‑71 (MQ=255)
cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt > 1:4676485/1‑71 (MQ=255)
|
AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCT > minE/1614422‑1614559
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
