Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R2	251	90.0	5200563	86.9%	4519289	59.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,614,492	T→C	29.6%	intergenic (‑117/+100)	eutH ← / ← eutG	predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,614,492	0	T	C	29.6%	44.5 / 17.2	27	intergenic (‑117/+100)	eutH/eutG	predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand): ref base T (8/11); new base C (3/5); total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCT  >  minE/1614422‑1614559

aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                                     >  1:3753546/1‑71 (MQ=38)

aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                                     >  1:270339/1‑71 (MQ=38)

   gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                                  <  1:3494483/71‑1 (MQ=255)

   gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                                  <  1:638910/71‑1 (MQ=255)

   gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                                  <  1:400448/71‑1 (MQ=255)

   gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                                  <  1:4344863/71‑1 (MQ=255)

     cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc                                                                  <  1:645112/69‑1 (MQ=11)

     cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc                                                                  <  1:284218/69‑1 (MQ=11)

     cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                                <  1:1768806/71‑1 (MQ=255)

     cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                                <  1:3801480/71‑1 (MQ=255)

     cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                                <  1:3337926/71‑1 (MQ=255)

     cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                                >  1:677866/1‑71 (MQ=255)

        aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc                                                             >  1:1454422/1‑71 (MQ=255)

        aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg                                                              >  1:4521302/1‑70 (MQ=255)

           cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc                                                           <  1:1875160/70‑1 (MQ=255)

                 aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                    >  1:3971104/1‑71 (MQ=255)

                  cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                                   >  1:711761/1‑71 (MQ=14)

                  cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                                   >  1:3481713/1‑71 (MQ=14)

                  cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                                   >  1:2517284/1‑71 (MQ=14)

                  cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                    <  1:2539557/70‑1 (MQ=255)

                                 aGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                                     <  1:322710/70‑1 (MQ=255)

                                      ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                               <  1:2912512/71‑1 (MQ=18)

                                      ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                               <  1:4770001/71‑1 (MQ=18)

                                      ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                               <  1:863468/71‑1 (MQ=18)

                                               gcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa                      >  1:1899153/1‑71 (MQ=255)

                                                       cGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCa              <  1:2609667/71‑1 (MQ=255)

                                                                cGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACACCTTATCCGCCCTACATGTGCAATCCCGTAg     >  1:3072994/1‑71 (MQ=255)

                                                                   cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc   >  1:4821198/1‑70 (MQ=255)

                                                                   cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt  >  1:3364875/1‑71 (MQ=255)

AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCT  >  minE/1614422‑1614559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A