mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | ||||||
|---|---|---|---|---|---|---|
| evidence | position | mutation | freq | annotation | gene | description |
| RA | 28,911 | T→C | 6.5% | I139I (ATT→ATC) | carB → | carbamoyl‑phosphate synthase large subunit |
| RA | 28,923 | C→T | 6.8% | T143T (ACC→ACT) | carB → | carbamoyl‑phosphate synthase large subunit |
| RA | 30,369 | T→C | 7.0% | D625D (GAT→GAC) | carB → | carbamoyl‑phosphate synthase large subunit |
| RA | 42,526 | C→T | 15.9% | G223G (GGC→GGT) | fixC → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| RA | 61,959 | A→G | 12.5% | Y500Y (TAT→TAC) | polB ← | DNA polymerase II |
| RA | 61,962 | A→G | 12.3% | F499F (TTT→TTC) | polB ← | DNA polymerase II |
| RA | 61,968 | A→G | 10.6% | N497N (AAT→AAC) | polB ← | DNA polymerase II |
| RA | 61,980 | T→C | 12.3% | K493K (AAA→AAG) | polB ← | DNA polymerase II |
| RA | 64,795 | G→T | 8.4% | G270G (GGC→GGA) | leuC ← | 3‑isopropylmalate isomerase subunit, dehydratase component |
| RA | 86,476 | T→A | 27.6% | T324T (ACT→ACA) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| RA | 86,479 | T→G | 25.0% | G325G (GGT→GGG) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| RA | 108,281 | C→G | 10.7% | L175L (CTC→CTG) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 108,284 | T→C | 10.7% | S176S (TCT→TCC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 108,287 | C→G | 9.8% | S177S (TCC→TCG) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 108,290 | T→C | 10.0% | Y178Y (TAT→TAC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 108,314 | A→C | 6.8% | E186D (GAA→GAC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 109,695 | C→T | 14.7% | H647Y (CAC→TAC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| RA | 123,547 | C→T | 12.5% | intergenic (+174/+28) | cueO → / ← gcd | multicopper oxidase (laccase)/glucose dehydrogenase |
| RA | 123,550 | A→G | 12.6% | intergenic (+177/+25) | cueO → / ← gcd | multicopper oxidase (laccase)/glucose dehydrogenase |
| RA | 143,709 | T→C | 12.5% | Y53C (TAC→TGC) | pcnB ← | poly(A) polymerase I |
| RA | 168,689 | G→T | 13.7% | intergenic (‑358/‑10) | map ← / → rpsB | methionine aminopeptidase/30S ribosomal subunit protein S2 |
| RA | 168,773 | G→A | 19.2% | P25P (CCG→CCA) | rpsB → | 30S ribosomal subunit protein S2 |
| RA | 168,776 | A→G | 16.5% | K26K (AAA→AAG) | rpsB → | 30S ribosomal subunit protein S2 |
| RA | 168,780 | A→G | 16.8% | K28E (AAG→GAG) ‡ | rpsB → | 30S ribosomal subunit protein S2 |
| RA | 168,781 | A→C | 16.9% | K28T (AAG→ACG) ‡ | rpsB → | 30S ribosomal subunit protein S2 |
| RA | 187,851 | G→A | 6.0% | A136T (GCG→ACG) | accA → | acetylCoA carboxylase, carboxytransferase, alpha subunit |
| RA | 204,511 | C→T | 100% | intergenic (+111/‑73) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| RA | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| RA | 214,925 | A→C | 89.2% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| RA | 214,931 | A→C | 89.1% | E14A (GAA→GCA) | dnaQ → | DNA polymerase III epsilon subunit |
| RA | 217,959 | A→G | 23.9% | intergenic (‑51/‑55) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| RA | 217,968 | C→T | 24.4% | intergenic (‑60/‑46) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| RA | 217,996 | A→T | 18.5% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| RA | 275,767 | C→G | 13.5% | T136T (ACG→ACC) | cyoE ← | protoheme IX farnesyltransferase |
| RA | 275,782 | G→A | 13.9% | H131H (CAC→CAT) | cyoE ← | protoheme IX farnesyltransferase |
| RA | 275,785 | G→A | 13.5% | R130R (CGC→CGT) | cyoE ← | protoheme IX farnesyltransferase |
| RA | 373,682 | G→T | 6.3% | A178S (GCG→TCG) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
| RA | 385,795 | T→C | 6.7% | C335C (TGT→TGC) | cstA → | carbon starvation protein |
| RA | 385,801 | G→C | 8.0% | A337A (GCG→GCC) | cstA → | carbon starvation protein |
| RA | 385,804 | G→C | 7.9% | V338V (GTG→GTC) | cstA → | carbon starvation protein |
| RA | 385,807 | T→G | 6.8% | S339S (TCT→TCG) | cstA → | carbon starvation protein |
| RA | 385,816 | T→C | 7.0% | H342H (CAT→CAC) | cstA → | carbon starvation protein |
| RA | 484,413 | C→T | 12.4% | R73R (CGC→CGT) | tolB → | periplasmic protein |
| RA | 484,420 | C→T | 12.9% | L76L (CTG→TTG) | tolB → | periplasmic protein |
| RA | 499,328 | C→T | 13.9% | V306I (GTT→ATT) | modF ← | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| RA | 518,462 | T→C | 8.1% | A162A (GCT→GCC) | bioC → | predicted methltransferase, enzyme of biotin synthesis |
| RA | 550,934 | T→C | 9.1% | K4K (AAA→AAG) | ybiV ← | predicted hydrolase |
| RA | 572,219 | A→G | 13.4% | I176I (ATT→ATC) | aqpZ ← | aquaporin |
| RA | 710,033 | G→C | 18.7% | intergenic (‑159/+102) | yceJ ← / ← yceO | predicted cytochrome b561/hypothetical protein |
| RA | 710,036 | G→C | 17.3% | intergenic (‑162/+99) | yceJ ← / ← yceO | predicted cytochrome b561/hypothetical protein |
| RA | 710,041 | G→A | 15.5% | intergenic (‑167/+94) | yceJ ← / ← yceO | predicted cytochrome b561/hypothetical protein |
| RA | 719,427 | C→T | 6.4% | A254V (GCC→GTC) | mviN → | predicted inner membrane protein |
| RA | 773,925 | C→T | 9.0% | L94L (TTG→TTA) | ymfC ← | 23S rRNA pseudouridine synthase |
| RA | 775,580 | A→G | 8.8% | K401K (AAA→AAG) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| RA | 775,607 | C→A | 9.4% | D410E (GAC→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| RA | 809,202 | T→C | 5.0% | K275K (AAA→AAG) | ychF ← | predicted GTP‑binding protein |
| RA | 826,746 | T→A | 11.0% | S381S (TCT→TCA) | ychP → | predicted invasin |
| RA | 949,284 | T→A | 5.1% | Y518F (TAT→TTT) | dos ← | cAMP phosphodiesterase, heme‑regulated |
| RA | 1,036,505 | G→A | 100% | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
| RA | 1,080,862 | A→G | 11.9% | N220N (AAT→AAC) | ydhV ← | predicted oxidoreductase |
| RA | 1,185,005 | G→A | 13.4% | V324I (GTT→ATT) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,185,007 | T→C | 13.3% | V324V (GTT→GTC) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,185,010 | C→T | 10.6% | Y325Y (TAC→TAT) | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,185,032 | T→C | 9.8% | L333L (TTG→CTG) | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,185,038 | A→G | 8.4% | I335V (ATT→GTT) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,185,040 | T→G | 8.4% | I335M (ATT→ATG) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| RA | 1,191,074 | A→C | 9.6% | intergenic (+415/+485) | yeaJ → / ← rnd | predicted diguanylate cyclase/ribonuclease D |
| RA | 1,223,810 | T→C | 11.1% | S795S (AGT→AGC) | yebT → | conserved hypothetical protein |
| RA | 1,305,362 | T→C | 15.1% | T233A (ACG→GCG) | yeeE ← | predicted inner membrane protein |
| RA | 1,305,959 | A→G | 6.7% | Y34H (TAT→CAT) | yeeE ← | predicted inner membrane protein |
| RA | 1,305,960 | C→T | 6.4% | M33I (ATG→ATA) | yeeE ← | predicted inner membrane protein |
| RA | 1,305,967 | C→T | 6.4% | R31H (CGC→CAC) | yeeE ← | predicted inner membrane protein |
| RA | 1,314,255 | T→C | 9.1% | H170H (CAT→CAC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| RA | 1,315,169 | C→T | 13.7% | N119N (AAC→AAT) | hisH → | imidazole glycerol phosphate synthase, glutamine amidotransferase subunit |
| RA | 1,319,252 | G→A | 12.2% | L214L (CTG→TTG) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,256 | G→A | 11.2% | N212N (AAC→AAT) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,262 | G→A | 10.1% | Y210Y (TAC→TAT) ‡ | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,263 | T→A | 9.7% | Y210F (TAC→TTC) ‡ | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,265 | C→T | 10.0% | A209A (GCG→GCA) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,268 | C→A | 9.9% | V208V (GTG→GTT) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,271 | G→T | 10.0% | R207R (CGC→CGA) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,319,277 | C→A | 9.3% | A205A (GCG→GCT) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| RA | 1,336,081 | G→A | 10.4% | G280G (GGC→GGT) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| RA | 1,336,113 | G→A | 10.4% | L270L (CTG→TTG) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| RA | 1,393,077 | T→C | 6.1% | K241E (AAA→GAA) | fruK ← | fructose‑1‑phosphate kinase |
| RA | 1,403,006 | G→A | 11.1% | P283P (CCG→CCA) | rtn → | conserved hypothetical protein |
| RA | 1,418,305 | T→C | 11.2% | I85T (ATC→ACC) | narP → | DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| RA | 1,488,632 | A→G | 8.5% | L132L (CTT→CTC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| RA | 1,488,647 | A→C | 9.5% | V127V (GTT→GTG) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| RA | 1,488,650 | G→A | 9.3% | G126G (GGC→GGT) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| RA | 1,488,719 | T→G | 10.0% | L103L (CTA→CTC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| RA | 1,498,221 | T→C | 13.8% | K541K (AAA→AAG) | nuoC ← | NADH:ubiquinone oxidoreductase, chain C,D |
| RA | 1,502,847 | A→T | 11.3% | intergenic (‑220/‑700) | lrhA ← / → yfbQ | DNA‑binding transcriptional repressor/predicted aminotransferase |
| RA | 1,515,674 | A→G | 7.8% | A114A (GCT→GCC) | cvpA ← | membrane protein required for colicin V production |
| RA | 1,614,452 | C→T | 7.2% | intergenic (‑77/+140) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,453 | G→C | 7.2% | intergenic (‑78/+139) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,454 | T→C | 7.3% | intergenic (‑79/+138) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,455 | A→T | 8.8% | intergenic (‑80/+137) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,459 | T→C | 10.6% | intergenic (‑84/+133) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,466 | G→A | 16.7% | intergenic (‑91/+126) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,480 | C→T | 24.2% | intergenic (‑105/+112) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,614,492 | T→C | 29.6% | intergenic (‑117/+100) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| RA | 1,635,083 | T→C | 13.4% | D407D (GAT→GAC) | acrD → | aminoglycoside/multidrug efflux system |
| RA | 1,635,086 | C→T | 12.4% | D408D (GAC→GAT) | acrD → | aminoglycoside/multidrug efflux system |
| RA | 1,635,104 | A→G | 10.5% | E414E (GAA→GAG) | acrD → | aminoglycoside/multidrug efflux system |
| RA | 1,658,871 | G→A | 12.7% | P357P (CCG→CCA) | ppx → | exopolyphosphatase |
| RA | 1,664,022 | A→G | 7.8% | R400R (CGT→CGC) | guaA ← | GMP synthetase |
| RA | 1,664,028 | C→G | 6.9% | L398L (CTG→CTC) ‡ | guaA ← | GMP synthetase |
| RA | 1,664,030 | G→C | 6.4% | L398V (CTG→GTG) ‡ | guaA ← | GMP synthetase |
| RA | 1,664,040 | C→G | 7.1% | P394P (CCG→CCC) | guaA ← | GMP synthetase |
| RA | 1,664,058 | A→G | 6.8% | G388G (GGT→GGC) | guaA ← | GMP synthetase |
| RA | 1,664,061 | A→G | 7.4% | I387I (ATT→ATC) | guaA ← | GMP synthetase |
| RA | 1,664,067 | A→G | 8.1% | R385R (CGT→CGC) | guaA ← | GMP synthetase |
| RA | 1,664,079 | T→C | 10.3% | K381K (AAA→AAG) | guaA ← | GMP synthetase |
| RA | 1,755,953 | G→A | 10.6% | D181N (GAC→AAC) | pssA → | phosphatidylserine synthase |
| RA | 1,755,958 | T→A | 9.7% | I182I (ATT→ATA) | pssA → | phosphatidylserine synthase |
| RA | 1,763,540 | A→G | 34.7% | noncoding (304/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,763,556 | T→C | 33.8% | noncoding (288/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,763,558 | G→C | 18.2% | noncoding (286/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,763,559 | G→T | 18.1% | noncoding (285/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,763,580 | G→T | 11.2% | noncoding (264/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,763,636 | A→G | 14.6% | noncoding (208/1542 nt) | rrsG ← | 16S ribosomal RNA |
| RA | 1,768,269 | A→G | 22.4% | T143T (ACT→ACC) | rluD ← | 23S rRNA pseudouridine synthase |
| RA | 1,804,090 | G→A | 84.1% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| RA | 1,829,982 | A→T | 9.8% | G186G (GGA→GGT) | pphB → | serine/threonine‑specific protein phosphatase 2 |
| RA | 1,830,030 | T→A | 6.5% | I202I (ATT→ATA) | pphB → | serine/threonine‑specific protein phosphatase 2 |
| RA | 1,844,947 | A→G | 8.5% | R46R (CGT→CGC) | cysN ← | sulfate adenylyltransferase, subunit 1 |
| RA | 1,844,959 | A→G | 7.8% | T42T (ACT→ACC) | cysN ← | sulfate adenylyltransferase, subunit 1 |
| RA | 1,858,847 | C→T | 18.6% | K245K (AAG→AAA) | cysI ← | sulfite reductase, beta subunit, NAD(P)‑binding |
| RA | 1,876,422 | A→G | 5.5% | G334G (GGT→GGC) | eno ← | enolase |
| RA | 1,876,425 | T→C | 5.5% | K333K (AAA→AAG) | eno ← | enolase |
| RA | 1,876,428 | T→C | 5.5% | E332E (GAA→GAG) ‡ | eno ← | enolase |
| RA | 1,876,430 | C→G | 5.7% | E332Q (GAA→CAA) ‡ | eno ← | enolase |
| RA | 1,876,434 | A→G | 6.4% | G330G (GGT→GGC) | eno ← | enolase |
| RA | 1,876,440 | T→C | 6.4% | K328K (AAA→AAG) | eno ← | enolase |
| RA | 1,876,461 | T→G | 6.0% | V321V (GTA→GTC) | eno ← | enolase |
| RA | 1,884,163 | G→A | 12.2% | A107V (GCA→GTA) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| RA | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| RA | 2,007,627 | T→A | 5.5% | S92C (AGC→TGC) | hybA ← | hydrogenase 2 4Fe‑4S ferredoxin‑type component |
| RA | 2,064,986 | C→T | 7.4% | N409N (AAC→AAT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,064,992 | T→C | 9.3% | G411G (GGT→GGC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,001 | A→G | 9.3% | K414K (AAA→AAG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,007 | T→G | 8.2% | V416V (GTT→GTG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,013 | A→G | 5.2% | K418K (AAA→AAG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,025 | C→T | 9.1% | R422R (CGC→CGT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,028 | T→C | 8.8% | R423R (CGT→CGC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,031 | T→C | 5.5% | G424G (GGT→GGC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,065,034 | C→T | 7.7% | Y425Y (TAC→TAT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| RA | 2,068,677 | A→G | 5.3% | N371N (AAT→AAC) | aer ← | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| RA | 2,155,529 | A→G | 14.3% | intergenic (+40/+146) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| RA | 2,155,567 | A→T | 7.3% | intergenic (+78/+108) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| RA | 2,253,051 | A→G | 41.7% | T1329T (ACT→ACC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,253,057 | C→T | 37.9% | E1327E (GAG→GAA) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,253,072 | C→G | 31.3% | A1322A (GCG→GCC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,253,075 | G→C | 31.3% | S1321S (TCC→TCG) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,740 | A→G | 10.8% | G766G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,746 | A→C | 12.2% | R764R (CGT→CGG) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,764 | T→C | 13.7% | P758P (CCA→CCG) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,767 | C→G | 13.5% | T757T (ACG→ACC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,770 | T→C | 15.1% | E756E (GAA→GAG) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,779 | G→C | 16.1% | S753S (TCC→TCG) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,254,785 | A→G | 15.9% | D751D (GAT→GAC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,256,930 | A→G | 40.0% | G36G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| RA | 2,266,165 | G→A | 16.4% | I93I (ATC→ATT) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,174 | T→C | 15.1% | K90K (AAA→AAG) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,177 | A→G | 15.9% | V89V (GTT→GTC) ‡ | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,179 | C→T | 15.9% | V89I (GTT→ATT) ‡ | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,180 | A→G | 16.1% | Y88Y (TAT→TAC) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,192 | C→G | 22.2% | G84G (GGG→GGC) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,195 | C→T | 22.2% | P83P (CCG→CCA) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,266,204 | T→A | 13.9% | V80V (GTA→GTT) | tufB ← | protein chain elongation factor EF‑Tu |
| RA | 2,314,904 | A→G | 7.6% | Q74Q (CAA→CAG) | glpK → | glycerol kinase |
| RA | 2,343,092 | C→T | 24.3% | H273H (CAC→CAT) | glnL → | sensory kinase in two‑component regulatory system with GlnG |
| RA | 2,359,867 | G→A | 45.3% | noncoding (1228/2905 nt) | rrlA ← | 23S ribosomal RNA |
| RA | 2,359,876 | C→T | 27.6% | noncoding (1219/2905 nt) | rrlA ← | 23S ribosomal RNA |
| RA | 2,385,964 | A→T | 13.4% | L56L (CTA→CTT) | metR → | DNA‑binding transcriptional activator, homocysteine‑binding |
| RA | 2,396,393 | T→G | 11.4% | V265V (GTA→GTC) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,394 | A→G | 11.7% | V265A (GTA→GCA) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,396 | C→G | 11.9% | V264V (GTG→GTC) | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,399 | G→C | 12.4% | S263S (TCC→TCG) | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,402 | T→G | 9.6% | V262V (GTA→GTC) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,403 | A→G | 10.6% | V262A (GTA→GCA) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,417 | T→C | 9.5% | K257K (AAA→AAG) | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,426 | G→C | 9.3% | R254R (CGC→CGG) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,427 | C→T | 9.2% | R254H (CGC→CAC) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,428 | G→T | 10.1% | R254S (CGC→AGC) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,435 | C→G | 8.8% | E251D (GAG→GAC) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,396,437 | C→T | 9.1% | E251K (GAG→AAG) ‡ | corA ← | magnesium/nickel/cobalt transporter |
| RA | 2,446,556 | A→C | 8.8% | pseudogene (1500/1647 nt) | ilvG ← | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| RA | 2,447,032 | C→A | 27.7% | pseudogene (1024/1647 nt) | ilvG ← | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| RA | 2,494,739 | G→A | 5.6% | P74P (CCG→CCA) | ibpB → | heat shock chaperone |
| RA | 2,518,526 | T→C | 16.0% | E183E (GAA→GAG) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 2,526,132 | T→C | 6.5% | A142A (GCT→GCC) | pyrE → | orotate phosphoribosyltransferase |
| RA | 2,526,156 | A→G | 6.6% | V150V (GTA→GTG) | pyrE → | orotate phosphoribosyltransferase |
| RA | 2,539,542 | A→G | 5.0% | N23D (AAT→GAT) | rfaJ → | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| RA | 2,545,256 | C→T | 5.9% | A81T (GCG→ACG) | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| RA | 2,545,260 | A→G | 5.1% | R79R (CGT→CGC) ‡ | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| RA | 2,545,261 | C→T | 5.2% | R79H (CGT→CAT) ‡ | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| RA | 2,545,265 | A→G | 5.7% | F78L (TTT→CTT) | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| RA | 2,595,746 | G→A | 7.5% | I284I (ATC→ATT) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,749 | G→A | 6.8% | V283V (GTC→GTT) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,752 | A→G | 7.9% | I282I (ATT→ATC) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,761 | G→T | 12.0% | P279P (CCC→CCA) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,764 | G→C | 12.0% | A278A (GCC→GCG) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,782 | A→T | 13.6% | G272G (GGT→GGA) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,785 | C→G | 12.6% | T271T (ACG→ACC) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,788 | A→G | 12.2% | N270N (AAT→AAC) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,791 | A→G | 11.1% | I269I (ATT→ATC) | arsB ← | arsenite/antimonite transporter |
| RA | 2,595,796 | C→A | 12.0% | A268S (GCG→TCG) | arsB ← | arsenite/antimonite transporter |
| RA | 2,611,527 | A→G | 9.7% | L141P (CTT→CCT) | nikB ← | nickel transporter subunit |
| RA | 2,682,413 | G→A | 15.5% | F433F (TTC→TTT) | yhgF ← | predicted transcriptional accessory protein |
| RA | 2,682,416 | C→T | 14.4% | E432E (GAG→GAA) | yhgF ← | predicted transcriptional accessory protein |
| RA | 2,682,425 | A→T | 14.8% | A429A (GCT→GCA) | yhgF ← | predicted transcriptional accessory protein |
| RA | 2,714,529 | T→C | 5.8% | intergenic (‑169/+93) | nirB ← / ← tsgA | nitrite reductase, large subunit, NAD(P)H‑binding/predicted transporter |
| RA | 2,749,136 | G→C | 8.3% | G403G (GGG→GGC) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,139 | C→G | 8.5% | T404T (ACC→ACG) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,142 | A→G | 8.2% | S405S (TCA→TCG) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,145 | G→C | 7.7% | L406L (CTG→CTC) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,148 | T→G | 6.9% | L407L (CTT→CTG) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,152 | G→A | 7.4% | V409I (GTT→ATT) ‡ | secY → | preprotein translocase membrane subunit |
| RA | 2,749,154 | T→C | 7.7% | V409V (GTT→GTC) ‡ | secY → | preprotein translocase membrane subunit |
| RA | 2,749,157 | T→G | 7.5% | V410V (GTT→GTG) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,160 | C→G | 6.8% | V411V (GTC→GTG) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,165 | T→C | 6.9% | I413T (ATT→ACT) ‡ | secY → | preprotein translocase membrane subunit |
| RA | 2,749,166 | T→G | 6.8% | I413M (ATT→ATG) ‡ | secY → | preprotein translocase membrane subunit |
| RA | 2,749,175 | T→C | 7.6% | F416F (TTT→TTC) | secY → | preprotein translocase membrane subunit |
| RA | 2,749,181 | T→A | 9.7% | A418A (GCT→GCA) | secY → | preprotein translocase membrane subunit |
| RA | 2,761,558 | T→C | 21.8% | L124L (CTT→CTC) | aroE → | dehydroshikimate reductase, NAD(P)‑binding |
| RA | 2,761,561 | C→T | 24.8% | I125I (ATC→ATT) | aroE → | dehydroshikimate reductase, NAD(P)‑binding |
| RA | 2,780,573 | A→C | 8.9% | P516P (CCA→CCC) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| RA | 2,780,576 | A→G | 8.6% | E517E (GAA→GAG) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| RA | 2,780,579 | T→C | 8.3% | D518D (GAT→GAC) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| RA | 2,807,677 | T→A | 5.7% | intergenic (+496/‑611) | tyrB → / → aphA | tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/acid phosphatase/phosphotransferase, class B, non‑specific |
| RA | 2,810,028 | T→C | 15.0% | P906P (CCA→CCG) | uvrA ← | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| RA | 2,818,696 | C→T | 6.5% | V112V (GTC→GTT) | nrfB → | nitrite reductase, formate‑dependent, penta‑heme cytochrome c |
| RA | 2,859,163 | A→G | 7.8% | L30L (CTA→CTG) | mutL → | methyl‑directed mismatch repair protein |
| RA | 2,880,895 | C→T | 8.4% | L71L (TTG→TTA) | ytfF ← | predicted inner membrane protein |
| RA | 2,935,934 | A→G | 6.4% | intergenic (‑144/‑73) | idnD ← / → idnK | L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive |
| RA | 2,950,841 | T→C | 7.5% | L113S (TTA→TCA) | bglJ → | DNA‑binding transcriptional regulator |
| RA | 2,965,079 | T→C | 11.3% | I187I (ATT→ATC) | deoA → | thymidine phosphorylase |
| RA | 2,965,083 | G→T | 10.0% | A189S (GCG→TCG) ‡ | deoA → | thymidine phosphorylase |
| RA | 2,965,085 | G→C | 10.8% | A189A (GCG→GCC) ‡ | deoA → | thymidine phosphorylase |
| RA | 2,965,091 | A→G | 10.4% | K191K (AAA→AAG) | deoA → | thymidine phosphorylase |
| RA | 2,965,094 | T→G | 8.2% | L192L (CTT→CTG) | deoA → | thymidine phosphorylase |
| RA | 2,965,095 | G→T | 6.7% | A193S (GCG→TCG) | deoA → | thymidine phosphorylase |
| RA | 2,965,099 | A→C | 6.9% | E194A (GAA→GCA) ‡ | deoA → | thymidine phosphorylase |
| RA | 2,965,100 | A→G | 6.9% | E194E (GAA→GAG) ‡ | deoA → | thymidine phosphorylase |
| RA | 2,965,103 | T→C | 7.0% | G195G (GGT→GGC) | deoA → | thymidine phosphorylase |
| RA | 2,965,109 | C→T | 7.4% | D197D (GAC→GAT) | deoA → | thymidine phosphorylase |
| RA | 2,965,130 | A→G | 11.1% | K204K (AAA→AAG) | deoA → | thymidine phosphorylase |
| RA | 2,969,927 | G→A | 17.5% | R161C (CGC→TGC) | lplA ← | lipoate‑protein ligase A |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | minE | 252012 | 252032 | 21 | 10 [7] | [7] 14 | malZ | maltodextrin glucosidase |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | minE | 183922 = | 64 (0.870) | 68 (1.110) +TTATAACA |
14/80 | NT | 59.5% | intergenic (+8/‑29) | rnhB/dnaE | ribonuclease HII, degrades RNA of DNA‑RNA hybrids/DNA polymerase III alpha subunit |
| ? | minE | = 296824 | 47 (0.640) | intergenic (+125/‑28) | cof/ybaO | thiamin pyrimidine pyrophosphate hydrolase/predicted DNA‑binding transcriptional regulator | |||||
| * | ? | minE | = 215622 | 56 (0.760) | 191 (5.950) +27 bp |
41/42 | NT | 87.5% | coding (732/732 nt) | dnaQ | DNA polymerase III epsilon subunit |
| ? | minE | 2705182 = | 69 (0.940) | intergenic (+106/‑1) | damX/dam | hypothetical protein/DNA adenine methylase | |||||
| * | ? | minE | = 242249 | 95 (1.300) | 9 (0.130) | 3/92 | NT | 9.0% | coding (1974/3147 nt) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| ? | minE | = 242319 | 92 (1.310) | coding (1904/3147 nt) | sbcC | exonuclease, dsDNA, ATP‑dependent | |||||
| * | ? | minE | 436259 = | 70 (0.950) | 342 (5.090) | 62/88 | NT | 87.6% | intergenic (‑156/‑29) | ybfF/seqA | conserved hypothetical protein/regulatory protein for replication initiation |
| ? | minE | = 2706018 | 33 (0.490) | coding (836/837 nt) | dam | DNA adenine methylase | |||||
| * | ? | minE | = 436833 | 83 (1.130) | 559 (7.620) | 72/96 | NT | 84.1% | coding (546/546 nt) | seqA | regulatory protein for replication initiation |
| ? | minE | 1803907 = | 128 (1.750) | intergenic (+63/‑28) | ygaH/mprA | predicted inner membrane protein/DNA‑binding transcriptional regulator | |||||