Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,747,012 |
A→G |
34.1% |
intergenic (+96/+113) |
srmB → / ← yfiE |
ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,747,012 | 0 | A | G | 34.1%
| 108.6
/ 69.5
| 82 | intergenic (+96/+113) | srmB/yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (18/36); new base G (13/15); total (31/51) |
| Fisher's exact test for biased strand distribution p-value = 3.37e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
ATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATA > minE/1746942‑1747073
|
aTTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAata > 1:2821384/1‑71 (MQ=255)
aaaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatatat < 1:3474954/70‑1 (MQ=255)
aaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTg < 1:796947/71‑1 (MQ=255)
actgatgacaaaggtaaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGa < 1:2292355‑M2/51‑1 (MQ=255)
actgatgacaaaggtaaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGa < 1:2818381‑M2/51‑1 (MQ=255)
gTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatata < 1:2024131/66‑1 (MQ=255)
gTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGaa < 1:2751358/71‑1 (MQ=255)
gTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGa < 1:3473074/70‑1 (MQ=255)
tGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt < 1:5074793/71‑1 (MQ=255)
cTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAttt > 1:1283976/1‑71 (MQ=255)
tgacaaaggtaaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTg < 1:1024386‑M2/56‑1 (MQ=255)
gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGc < 1:1178496/70‑1 (MQ=255)
gCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGc < 1:4691263/70‑1 (MQ=255)
aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGa > 1:3973708/1‑70 (MQ=255)
aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAt > 1:1613768/1‑71 (MQ=255)
aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAt > 1:4283624/1‑71 (MQ=255)
aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAt > 1:1474693/1‑71 (MQ=255)
cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:947765/71‑1 (MQ=255)
cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:5576898/71‑1 (MQ=255)
gtaaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGaa < 1:1234328‑M2/52‑1 (MQ=255)
gtaaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAAttt < 1:289721‑M2/55‑1 (MQ=255)
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCt < 1:3997055/71‑1 (MQ=255)
aaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAAtt > 1:392972‑M2/6‑59 (MQ=255)
aaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGc > 1:4729318‑M2/6‑62 (MQ=255)
aaaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCAc > 1:4841015‑M2/6‑64 (MQ=255)
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:5417556/71‑1 (MQ=255)
aaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGt < 1:3822201‑M2/67‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:1747399/71‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:5319875/71‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATAAATTGTATTTGCACGATCTTGt < 1:2638081/71‑1 (MQ=255)
aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg > 1:1255450/1‑69 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGa < 1:4109525/61‑1 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCAAGa < 1:1051562/61‑1 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTg > 1:1216731/1‑56 (MQ=14)
tGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGc > 1:3313732/1‑57 (MQ=14)
ccTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg < 1:3962906/71‑1 (MQ=255)
ccTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCg < 1:337807/71‑1 (MQ=14)
cTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg > 1:3841755/1‑70 (MQ=255)
tGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:5583573/62‑1 (MQ=14)
gATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGc < 1:3236889/52‑1 (MQ=255)
gATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGa > 1:2652614/1‑70 (MQ=14)
aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4543787/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4336819/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:2012413/70‑1 (MQ=14)
tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa < 1:5656641/70‑1 (MQ=255)
tGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa < 1:3724856/71‑1 (MQ=14)
cgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAg < 1:537265/70‑1 (MQ=255)
cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:1601060/57‑1 (MQ=14)
cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:2101676/57‑1 (MQ=14)
gcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGc < 1:1839796/71‑1 (MQ=14)
cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:211605/71‑1 (MQ=255)
cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:3888979/71‑1 (MQ=255)
cTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGa < 1:5555709/40‑1 (MQ=14)
cTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGa < 1:3492326/40‑1 (MQ=14)
cTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAAt > 1:4402383/1‑42 (MQ=14)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:1050744/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:627051/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:3487589/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:1918981/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:3085331/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAatatat > 1:2099620/1‑35 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAtt < 1:3675104/41‑1 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAtt < 1:1440307/41‑1 (MQ=255)
aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAtt < 1:5037317/41‑1 (MQ=255)
aCGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa > 1:4356994/1‑65 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:3622613/1‑57 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:2093406/1‑57 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3799248/1‑70 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3952435/1‑70 (MQ=14)
gCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTg > 1:325515/1‑41 (MQ=255)
gCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTg > 1:1157651/1‑41 (MQ=255)
cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAc < 1:116695/71‑1 (MQ=255)
ttATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt < 1:1500793/67‑1 (MQ=255)
tATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTAcgc > 1:2397133/1‑71 (MQ=11)
cAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:2219218/1‑70 (MQ=255)
aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3075374/71‑1 (MQ=255)
ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:877077/55‑1 (MQ=255)
ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:5315591/55‑1 (MQ=255)
cTACGTGGCTCATGCAATATATTGAATTTGCACGATc > 1:2937576/1‑37 (MQ=255)
cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc > 1:3182663/1‑52 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4465721/1‑48 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4243018/1‑48 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:4993722/64‑1 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3191174/64‑1 (MQ=255)
tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:4811377/1‑70 (MQ=255)
tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:2646128/1‑70 (MQ=255)
aTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATa > 1:952675/1‑69 (MQ=255)
tGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2940977/50‑1 (MQ=255)
gCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2895432/49‑1 (MQ=255)
cAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:5574907/1‑38 (MQ=255)
aaTATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:1655501/47‑1 (MQ=255)
tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa < 1:2996414/61‑1 (MQ=255)
atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa > 1:3934492/1‑59 (MQ=255)
|
ATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATA > minE/1746942‑1747073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
