Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,164 |
A→G |
25.0% |
G93G (GGT→GGC) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,164 | 0 | A | G | 25.0%
| 179.6
/ 65.6
| 112 | G93G (GGT→GGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (42/42); new base G (24/4); total (66/46) |
| Fisher's exact test for biased strand distribution p-value = 8.09e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01 |
Only 100 of 114 total aligned reads displayed.
ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAG > minE/1934097‑1934231
|
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:296627/1‑69 (MQ=255)
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2943895/1‑71 (MQ=255)
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2809910/1‑71 (MQ=255)
aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:4767942/1‑70 (MQ=255)
aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:2388906/1‑70 (MQ=255)
aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:3405972/1‑70 (MQ=255)
aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:3426560/1‑70 (MQ=255)
tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:418436/1‑70 (MQ=255)
tttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:530027/1‑71 (MQ=255)
ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcc > 1:4939613/1‑67 (MQ=255)
ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3316314/1‑70 (MQ=255)
ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3243048/1‑70 (MQ=255)
tttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCa < 1:2318093/70‑1 (MQ=255)
taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:3441751/3‑70 (MQ=255)
taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:2601234/3‑70 (MQ=255)
gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:50296/70‑1 (MQ=255)
gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:1604905/70‑1 (MQ=255)
aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc < 1:2636232/71‑1 (MQ=255)
aCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATcc > 1:3362005/1‑71 (MQ=255)
cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg < 1:2561669/69‑1 (MQ=255)
cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:4000042/1‑71 (MQ=255)
gTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:1469185/71‑1 (MQ=255)
tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:4041612/65‑1 (MQ=255)
tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:218029/1‑70 (MQ=255)
tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:4648808/1‑70 (MQ=255)
ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:4515001/1‑71 (MQ=255)
ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:3361187/1‑71 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTCCAGCTGAATGCGACCGCCa > 1:1493144/1‑52 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc > 1:1558446/1‑55 (MQ=255)
ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:3751635/55‑1 (MQ=255)
tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa > 1:2600601/1‑39 (MQ=255)
tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa > 1:1827217/1‑39 (MQ=255)
tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:2419192/1‑71 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:4034452/1‑39 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:30936/1‑39 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTTCAGGGTAACGAAAGACGc < 1:4037873/70‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:315032/70‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:3594207/1‑71 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:43532/1‑71 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:830366/1‑71 (MQ=255)
gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt < 1:2600185/71‑1 (MQ=255)
ggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa < 1:4818060/71‑1 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAAc > 1:5595980/1‑40 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1369846/71‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat < 1:4953092/71‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1506555/68‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:4254397/68‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1500069/68‑1 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:2967745/1‑44 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGa > 1:3729867/1‑55 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:4089260/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:2305793/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5635555/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:3670634/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5192515/1‑71 (MQ=255)
aCGGGCAACGTACAGATGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2103692/1‑68 (MQ=255)
cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:4010170/4‑70 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5206876/49‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1083991/49‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3553945/49‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5608964/49‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3261681/65‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5431537/1‑70 (MQ=255)
ttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:1882957/3‑71 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacga < 1:4257391/71‑1 (MQ=255)
gCAACGTACAGTTGAATCCGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2586352/1‑59 (MQ=255)
gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:3486407/1‑59 (MQ=255)
gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2435601/1‑59 (MQ=255)
cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg > 1:1178934/1‑71 (MQ=255)
aaCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:1086633/59‑1 (MQ=255)
aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:3189115/1‑71 (MQ=255)
aCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt < 1:1442921/71‑1 (MQ=255)
cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:2923742/1‑70 (MQ=255)
gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:2922051/43‑1 (MQ=255)
gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5027833/41‑1 (MQ=255)
tACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat < 1:459105/71‑1 (MQ=255)
tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:3633276/46‑1 (MQ=255)
tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2695632/1‑71 (MQ=255)
tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:192176/71‑1 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg > 1:1351298/1‑61 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:1692253/1‑46 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2369524/1‑71 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2739834/1‑71 (MQ=255)
aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:4756993/71‑1 (MQ=255)
tGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:65613/1‑71 (MQ=255)
gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt < 1:2181078/43‑1 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2526588/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:1549295/1‑70 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:3245899/70‑1 (MQ=255)
aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:3626458/47‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:4012404/70‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:5474961/48‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:2251878/48‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:5491071/1‑55 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:3691850/1‑55 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:63102/54‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:759894/54‑1 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:4926516/1‑70 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3759086/1‑70 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:1058439/1‑70 (MQ=255)
|
ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAG > minE/1934097‑1934231
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
