Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,164 |
A→G |
9.7% |
G93G (GGT→GGC) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,164 | 0 | A | G | 9.7%
| 248.0
/ 14.2
| 93 | G93G (GGT→GGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (46/38); new base G (6/3); total (52/41) |
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAA > minE/1934097‑1934234
|
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAcc > 1:143681/1‑70 (MQ=255)
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:869084/1‑71 (MQ=255)
aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:770755/1‑71 (MQ=255)
tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:3141339/1‑70 (MQ=255)
tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:1670600/1‑70 (MQ=255)
gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:1929597/70‑1 (MQ=255)
gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt > 1:753054/1‑71 (MQ=255)
aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt < 1:305061/70‑1 (MQ=255)
tgatCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTg > 1:2110839/4‑71 (MQ=255)
tGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg > 1:427440/1‑71 (MQ=255)
cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg < 1:78737/69‑1 (MQ=255)
cTCGTTATAAACGCCTTCCGGGAGATCCTCACGGGCAACTTACAGCTGAATGCGACCGCCAACGTCCTg < 1:1628814/69‑1 (MQ=255)
tCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa < 1:3229031/70‑1 (MQ=255)
tCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCaa > 1:3533858/1‑70 (MQ=255)
cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:605132/1‑71 (MQ=255)
ttATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt < 1:3138423/71‑1 (MQ=255)
tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:84639/1‑70 (MQ=255)
ataAACGCCTTCCGGGAGATTGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:1146836/1‑71 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1110733/71‑1 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:414129/71‑1 (MQ=255)
aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1251231/71‑1 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAcc < 1:3948456/47‑1 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:2067766/1‑49 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCaa > 1:3085135/1‑52 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCaa > 1:20534/1‑52 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCaa > 1:2360674/1‑52 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCt > 1:1558403/1‑58 (MQ=255)
aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2757308/1‑70 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:1195468/1‑69 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2351674/1‑69 (MQ=255)
cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:3482992/1‑69 (MQ=255)
cTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc > 1:3822468/1‑71 (MQ=255)
tCCGGGAGGTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:1264911/1‑55 (MQ=255)
tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:1887158/1‑55 (MQ=255)
tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:4883/1‑55 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGTATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:1339389/70‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:1693437/69‑1 (MQ=255)
ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:1586997/1‑71 (MQ=255)
gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3897061/61‑1 (MQ=255)
gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt > 1:2888995/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:3931835/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:1706607/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAAAGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:436605/71‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2413681/70‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATACGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa > 1:3835260/1‑71 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1893285/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:3817657/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1682024/57‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:46477/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:654561/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3872507/71‑1 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1667779/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1019782/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:2058786/1‑70 (MQ=255)
gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1479895/1‑67 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:2661970/46‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:2197201/52‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:874430/1‑70 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2383178/1‑70 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3682362/1‑52 (MQ=255)
cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3636070/1‑52 (MQ=255)
aCGTGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1721183/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTa > 1:801775/1‑46 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa > 1:314147/1‑52 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2946840/71‑1 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3568748/71‑1 (MQ=255)
cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:1342083/4‑70 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata > 1:2664461/1‑65 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATTATacga < 1:3212337/71‑1 (MQ=255)
gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAAc > 1:373295/1‑44 (MQ=255)
gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg < 1:1001202/71‑1 (MQ=255)
cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1176573/47‑1 (MQ=255)
cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc < 1:3945102/71‑1 (MQ=255)
cGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:2175408/1‑50 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:1685103/1‑46 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:3091605/1‑70 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2562071/1‑70 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1020139/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1719053/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:146099/70‑1 (MQ=255)
aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCTATAATACGACGGGTcatcat < 1:1842042/71‑1 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:2098882/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:1474238/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcat < 1:3442194/68‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACggg < 1:2773660/62‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3422759/70‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3424822/70‑1 (MQ=255)
tGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:2419705/58‑1 (MQ=255)
gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:613890/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2435959/1‑71 (MQ=255)
aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg < 1:1966525/70‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:1549006/70‑1 (MQ=255)
aTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc < 1:3845564/70‑1 (MQ=255)
aCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:1676818/71‑1 (MQ=255)
|
ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAA > minE/1934097‑1934234
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
