Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,200 |
A→C |
28.9% |
G81G (GGT→GGG) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,200 | 0 | A | C | 28.9%
| 82.9
/ 69.0
| 83 | G81G (GGT→GGG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (33/26); new base C (13/11); total (46/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 |
GGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > minE/1934130‑1934269
|
ggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa < 1:4818060/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3923450/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1369846/71‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat < 1:4953092/71‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:4254397/68‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1506555/68‑1 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1500069/68‑1 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:2305793/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:3670634/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:4089260/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5192515/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5635555/1‑71 (MQ=255)
aCGGGCAACGTACAGATGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2103692/1‑68 (MQ=255)
cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:4010170/4‑70 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3261681/65‑1 (MQ=255)
cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5431537/1‑70 (MQ=255)
ttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:1882957/3‑71 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacga < 1:4257391/71‑1 (MQ=255)
gCAACGTACAGTTGAATCCGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2586352/1‑59 (MQ=255)
gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2435601/1‑59 (MQ=255)
gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:3486407/1‑59 (MQ=255)
gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:97317/1‑69 (MQ=255)
cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg > 1:1178934/1‑71 (MQ=255)
aaCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:1086633/59‑1 (MQ=255)
aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:1821742/1‑71 (MQ=255)
aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:3189115/1‑71 (MQ=255)
aCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt < 1:1442921/71‑1 (MQ=255)
cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:2923742/1‑70 (MQ=255)
gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca < 1:2699140/71‑1 (MQ=255)
tACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat < 1:459105/71‑1 (MQ=255)
tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:192176/71‑1 (MQ=255)
tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2695632/1‑71 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg > 1:1351298/1‑61 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2369524/1‑71 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2739834/1‑71 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:5568952/70‑1 (MQ=255)
aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:4756993/71‑1 (MQ=255)
tGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:65613/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2526588/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2287907/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:1549295/1‑70 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:3245899/70‑1 (MQ=255)
aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:3626458/47‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:4012404/70‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:4738092/69‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:5474961/48‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:2251878/48‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:3691850/1‑55 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:5491071/1‑55 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:759894/54‑1 (MQ=255)
tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:63102/54‑1 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:1058439/1‑70 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:4926516/1‑70 (MQ=255)
cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3759086/1‑70 (MQ=255)
gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:932022/1‑68 (MQ=255)
gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:4279799/1‑68 (MQ=255)
gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:5591560/1‑68 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:3067599/1‑40 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1209557/1‑70 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:587097/71‑1 (MQ=255)
aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:3508765/63‑1 (MQ=255)
cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5157556/1‑65 (MQ=255)
cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5169788/1‑65 (MQ=255)
atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:5623845/63‑2 (MQ=255)
atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:4339776/63‑2 (MQ=255)
atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:234300/63‑2 (MQ=255)
ccTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:3715333/62‑2 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3961549/70‑2 (MQ=255)
tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:5539454/1‑70 (MQ=255)
gggTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:5414647/40‑1 (MQ=255)
ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:5377813/71‑1 (MQ=255)
tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:4153184/1‑49 (MQ=255)
tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:5176971/1‑49 (MQ=255)
aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:4978297/1‑71 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3304856/1‑39 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:1099062/1‑48 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:810261/1‑48 (MQ=255)
gAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:2343918/1‑70 (MQ=255)
aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:3316697/1‑71 (MQ=255)
aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2878285/71‑1 (MQ=255)
aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4023622/69‑1 (MQ=255)
ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt > 1:4066856/2‑71 (MQ=255)
gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt < 1:2252670/57‑2 (MQ=255)
gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:2613270/1‑71 (MQ=255)
tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct > 1:3144320/1‑69 (MQ=255)
tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:1295208/54‑1 (MQ=255)
tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:2759822/70‑1 (MQ=255)
aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:776813/70‑1 (MQ=255)
|
GGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > minE/1934130‑1934269
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
