Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,934,200 |
A→C |
15.5% |
G81G (GGT→GGG) |
lysS ← |
lysine tRNA synthetase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,934,200 | 0 | A | C | 15.5%
| 161.1
/ 33.8
| 84 | G81G (GGT→GGG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (35/36); new base C (6/7); total (41/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 |
GATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTC > minE/1934133‑1934268
|
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:3931835/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:1706607/1‑70 (MQ=255)
gaTCGTCACGGGCAACGTACAGCTGAAAGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:436605/71‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2413681/70‑1 (MQ=255)
aTCGTCACGGGCAACGTACAGCTGAATACGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa > 1:3835260/1‑71 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3872507/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:654561/71‑1 (MQ=255)
tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:46477/71‑1 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1019782/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:2058786/1‑70 (MQ=255)
cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1667779/1‑70 (MQ=255)
gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1479895/1‑67 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:874430/1‑70 (MQ=255)
tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2383178/1‑70 (MQ=255)
aCGTGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1721183/1‑71 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2946840/71‑1 (MQ=255)
aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3568748/71‑1 (MQ=255)
cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:1342083/4‑70 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata > 1:2664461/1‑65 (MQ=255)
gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATTATacga < 1:3212337/71‑1 (MQ=255)
gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg < 1:1001202/71‑1 (MQ=255)
cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc < 1:3945102/71‑1 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:3091605/1‑70 (MQ=255)
aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2562071/1‑70 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1719053/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1020139/70‑1 (MQ=255)
cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:146099/70‑1 (MQ=255)
aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCTATAATACGACGGGTcatcat < 1:1842042/71‑1 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:2098882/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:1474238/2‑69 (MQ=255)
ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcat < 1:3442194/68‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACggg < 1:2773660/62‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3424822/70‑1 (MQ=255)
cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3422759/70‑1 (MQ=255)
tGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:2419705/58‑1 (MQ=255)
gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:613890/1‑71 (MQ=255)
gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2435959/1‑71 (MQ=255)
aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg < 1:1966525/70‑1 (MQ=255)
aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:1549006/70‑1 (MQ=255)
aTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc < 1:3845564/70‑1 (MQ=255)
aCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:1676818/71‑1 (MQ=255)
cGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:3731146/71‑1 (MQ=255)
gcaaaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1476048/4‑71 (MQ=255)
gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAAgg > 1:1291757/1‑71 (MQ=255)
gCCAACGTCCTGCA‑GGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:1437688/71‑1 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata < 1:3426055/42‑1 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:3403473/1‑71 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:2043395/71‑1 (MQ=255)
aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa > 1:1720525/1‑61 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:1275403/1‑39 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3071995/1‑55 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:2494688/1‑55 (MQ=255)
aCGTCCTGCAGGGTAACGAAAGACGATTTACCCataata > 1:879284/1‑39 (MQ=255)
tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2141904/71‑1 (MQ=255)
tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2322979/71‑1 (MQ=255)
tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:2844694/71‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1274658/70‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:2069430/70‑1 (MQ=255)
cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa < 1:2120878/71‑1 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:1776962/70‑2 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3842180/70‑2 (MQ=255)
cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACAGAGACTTcaa > 1:1040718/1‑69 (MQ=255)
tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt < 1:1167715/67‑1 (MQ=255)
cAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat > 1:327039/1‑45 (MQ=255)
aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:258544/1‑71 (MQ=255)
aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:598419/1‑71 (MQ=255)
ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:3160277/71‑1 (MQ=255)
tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1337919/62‑1 (MQ=255)
aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:78977/52‑1 (MQ=255)
aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:3533358/1‑71 (MQ=255)
aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:3665650/1‑62 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:696461/1‑64 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:3529402/1‑66 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt < 1:3849325/71‑1 (MQ=255)
cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:3248566/1‑71 (MQ=255)
aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGAc > 1:1816200/1‑52 (MQ=255)
aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:66336/1‑70 (MQ=255)
gaggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAg > 1:2529101/4‑71 (MQ=255)
gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc < 1:3302059/71‑1 (MQ=255)
gCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttc < 1:3534421/71‑1 (MQ=255)
cTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct < 1:3938307/71‑1 (MQ=255)
ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:2104523/1‑50 (MQ=255)
ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:2433148/1‑50 (MQ=255)
ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc > 1:3348784/1‑71 (MQ=255)
|
GATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTC > minE/1934133‑1934268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
