Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,539 T→C 24.8% A353A (GCA→GCG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5390TC24.8% 304.0 / 109.1 181A353A (GCA→GCG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (71/65);  new base C (32/13);  total (103/78)
Fisher's exact test for biased strand distribution p-value = 3.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 181 total aligned reads displayed.

TAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAG  >  minE/2595472‑2595607
                                                                   |                                                                    
tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                                   <  1:848510/71‑1 (MQ=255)
  aTTTTCGGTCCCAAATCTCAGCCAATCACATTGGCATAAACAATCTATTCTTTGATAACGCCAGATGcc                                                                   >  1:5660729/1‑69 (MQ=255)
   ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                 <  1:3674096/70‑1 (MQ=255)
   ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                 <  1:2214288/70‑1 (MQ=255)
    tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGACAGATGCCGtg                                                                <  1:1286167/70‑1 (MQ=255)
      tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                                              >  1:803359/1‑70 (MQ=255)
           cctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc                                                        <  1:2033291/68‑1 (MQ=255)
           cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                                   >  1:50638/1‑60 (MQ=255)
            ctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc                                                        >  1:2891717/3‑70 (MQ=255)
            ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                        <  1:3367170/70‑1 (MQ=255)
            ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                        <  1:3949922/70‑1 (MQ=255)
             taaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                                      >  1:3565030/2‑71 (MQ=255)
             cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                      <  1:3422265/71‑1 (MQ=255)
              aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                               >  1:1087065/1‑61 (MQ=255)
              aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                     <  1:833011/71‑1 (MQ=255)
              aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                     >  1:4870513/1‑71 (MQ=255)
              aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                     <  1:4455755/71‑1 (MQ=255)
               aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                                  <  1:131323/57‑1 (MQ=255)
               aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                    <  1:911476/71‑2 (MQ=255)
               aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                     >  1:2903195/1‑70 (MQ=255)
                   gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                 <  1:3386210/70‑1 (MQ=255)
                   gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                 <  1:4587681/70‑1 (MQ=255)
                   gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc                                                 <  1:871666/70‑1 (MQ=255)
                   acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGAc                                                 <  1:3159032/69‑1 (MQ=255)
                   acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACa                                                <  1:214923/70‑1 (MQ=255)
                     aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                 <  1:2764875/52‑1 (MQ=255)
                      gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                                             >  1:4631505/1‑71 (MQ=255)
                      gCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg                                             <  1:2923227/71‑1 (MQ=255)
                       ccAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgc                                            <  1:3262228/71‑1 (MQ=255)
                       ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                               >  1:3954659/1‑52 (MQ=255)
                       ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                               >  1:416460/1‑52 (MQ=255)
                       ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                            >  1:4701257/1‑71 (MQ=255)
                          aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                  >  1:66522/1‑62 (MQ=255)
                          aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                  >  1:595489/1‑62 (MQ=255)
                          aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                  >  1:2176540/1‑62 (MQ=255)
                          aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                  >  1:1608917/1‑62 (MQ=255)
                          aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                         <  1:2079645/71‑1 (MQ=255)
                           tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa                                                  >  1:2221376/1‑61 (MQ=255)
                           tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCa                                        <  1:2781269/71‑1 (MQ=255)
                            cacaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                                                >  1:2658977/1‑45 (MQ=255)
                            cacaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                                                >  1:2939914/1‑45 (MQ=255)
                            cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                            >  1:1351659/1‑50 (MQ=255)
                            cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                       >  1:464410/1‑71 (MQ=255)
                            cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                       >  1:5013667/1‑71 (MQ=255)
                             acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                           >  1:3832121/1‑65 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                       <  1:466127/70‑1 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      <  1:5472608/71‑1 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      <  1:1037860/71‑1 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      >  1:3459158/1‑71 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      <  1:3254161/71‑1 (MQ=255)
                             acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      >  1:2308503/1‑71 (MQ=255)
                              caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                     <  1:1634087/71‑1 (MQ=255)
                              caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                      <  1:1966156/70‑1 (MQ=255)
                               aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                  >  1:168534/1‑57 (MQ=255)
                               aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                  >  1:5175147/1‑57 (MQ=255)
                               aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                    <  1:4849085/71‑1 (MQ=255)
                                ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                                      >  1:79368/1‑52 (MQ=255)
                                ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                     >  1:2988091/1‑53 (MQ=255)
                                ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                     >  1:5379357/1‑53 (MQ=255)
                                ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                   <  1:3909527/71‑1 (MQ=255)
                                 tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                      >  1:2678707/1‑51 (MQ=255)
                                 tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                  >  1:1689067/1‑55 (MQ=255)
                                  ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                      >  1:3142306/1‑50 (MQ=255)
                                  ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                      >  1:3308327/1‑50 (MQ=255)
                                  ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                  <  1:1472548/70‑1 (MQ=255)
                                  ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                  <  1:4262823/70‑1 (MQ=255)
                                   gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                 <  1:2396974/70‑1 (MQ=255)
                                      tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                 <  1:2747557/35‑1 (MQ=255)
                                      tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                               >  1:5394825/1‑53 (MQ=255)
                                      tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                             >  1:1124572/1‑71 (MQ=255)
                                       aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                >  1:1249439/1‑67 (MQ=255)
                                       aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                            >  1:2560031/1‑71 (MQ=255)
                                        aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                           >  1:2718291/1‑55 (MQ=255)
                                        aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                           >  1:3736814/1‑55 (MQ=255)
                                        aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                            <  1:4005571/70‑1 (MQ=255)
                                        aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                           >  1:1911825/1‑71 (MQ=255)
                                        aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc                                     >  1:4243777/1‑61 (MQ=255)
                                           cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                        <  1:769114/39‑1 (MQ=255)
                                           cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                        <  1:4131968/39‑1 (MQ=255)
                                            aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                      >  1:4003632/1‑40 (MQ=255)
                                               gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat                     >  1:300896/4‑70 (MQ=255)
                                               gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt                                                     >  1:5639749/1‑38 (MQ=255)
                                               gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                    <  1:4176290/71‑1 (MQ=255)
                                                   cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                  <  1:1743118/69‑1 (MQ=255)
                                                    tttGATAACGCCAGATGCCGTGCTGCCATCAATGGACa                                                >  1:2753541/1‑38 (MQ=255)
                                                    tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                 <  1:1311838/69‑1 (MQ=255)
                                                     ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                          >  1:1388473/1‑43 (MQ=255)
                                                     ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                          >  1:2577139/1‑43 (MQ=255)
                                                      tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                             >  1:389031/1‑55 (MQ=255)
                                                        aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                  >  1:5610185/1‑64 (MQ=255)
                                                         cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc                <  1:2852098/63‑1 (MQ=255)
                                                           aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat              <  1:5301797/65‑1 (MQ=255)
                                                           aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga        <  1:1448564/71‑1 (MQ=255)
                                                           aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag         <  1:364533/70‑1 (MQ=255)
                                                             gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca            >  1:1893540/1‑63 (MQ=255)
                                                             gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                    >  1:1560367/1‑41 (MQ=255)
                                                             gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                    >  1:2004573/1‑41 (MQ=255)
                                                             gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag      <  1:1003764/71‑1 (MQ=255)
                                                              ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                               <  1:2483012/45‑1 (MQ=255)
                                                                 gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag  <  1:5283863/71‑1 (MQ=255)
                                                                   |                                                                    
TAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAG  >  minE/2595472‑2595607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A