Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I0 R1	2169	63.0	3952800	76.2%	3012033	62.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,595,539	T→C	15.8%	A353A (GCA→GCG)	arsB ←	arsenite/antimonite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,595,539	0	T	C	15.8%	280.7 / 42.4	120	A353A (GCA→GCG)	arsB	arsenite/antimonite transporter
Reads supporting (aligned to +/- strand): ref base T (52/49); new base C (13/6); total (65/55)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 122 total aligned reads displayed.

GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGA  >  minE/2595471‑2595609

gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc                                                                      >  1:3629754/1‑71 (MQ=255)

  gaTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg                                                                    >  1:2310222/2‑71 (MQ=255)

  aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                                    >  1:3548375/1‑71 (MQ=255)

   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt                                                                   >  1:3657593/1‑71 (MQ=255)

   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg                                                                    <  1:3725864/70‑1 (MQ=255)

   aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                   >  1:3045419/1‑71 (MQ=255)

    ttttCGGGCCTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                                                  <  1:281000/71‑2 (MQ=255)

      ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                                                >  1:1834918/1‑71 (MQ=255)

      ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct                                                                >  1:1318215/1‑71 (MQ=255)

       tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                               >  1:1079007/1‑71 (MQ=255)

       tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                                               >  1:1335928/1‑70 (MQ=255)

        cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc                                                               <  1:1014757/70‑2 (MQ=255)

         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                              >  1:1098596/1‑70 (MQ=255)

         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc                                                              <  1:2603902/70‑1 (MQ=255)

         ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc                                                             <  1:2138816/71‑1 (MQ=255)

          gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                            <  1:3081292/71‑1 (MQ=255)

          gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                            <  1:3379826/71‑1 (MQ=255)

           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                                                  <  1:3699881/64‑1 (MQ=255)

           tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg                                                                  <  1:1221579/64‑1 (MQ=255)

            cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg                                                               >  1:3733789/1‑66 (MQ=255)

              cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt                                                                        >  1:1126735/1‑55 (MQ=255)

               aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt                                                       >  1:3493069/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                                 >  1:96736/1‑60 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                                 >  1:3524820/1‑60 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:1919377/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:2405114/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:899769/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      <  1:2865012/71‑1 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:2105044/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      <  1:2090554/71‑1 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:1132875/1‑71 (MQ=255)

                aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:1673561/1‑71 (MQ=255)

                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta                                                                  >  1:743546/1‑58 (MQ=255)

                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                      >  1:1814704/1‑70 (MQ=255)

                aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc                                                      >  1:453421/1‑70 (MQ=255)

                 aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                            >  1:3412466/1‑64 (MQ=255)

                 aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa                                                        <  1:3923911/68‑1 (MQ=255)

                   cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa                                                    >  1:810901/1‑70 (MQ=255)

                     cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa                                                 <  1:2174292/71‑1 (MQ=255)

                      aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc                                                >  1:2383520/1‑69 (MQ=255)

                       gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg                                               <  1:925202/71‑1 (MQ=255)

                        ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg                                               >  1:2386721/1‑70 (MQ=255)

                         gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg                                             <  1:1149530/70‑1 (MQ=255)

                         cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                             >  1:3797257/1‑70 (MQ=255)

                         cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                              <  1:1894529/70‑1 (MQ=255)

                          aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac                                            >  1:782482/1‑69 (MQ=255)

                          aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca                                             <  1:2834251/70‑2 (MQ=255)

                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc                                                          <  1:3800795/57‑1 (MQ=255)

                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                            >  1:3837660/1‑71 (MQ=255)

                          aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                            <  1:301922/71‑1 (MQ=255)

                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc                                                                     <  1:794708/45‑1 (MQ=255)

                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt                                                                   >  1:2633605/1‑47 (MQ=255)

                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa                                                            <  1:1290411/54‑1 (MQ=255)

                           aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                            >  1:2994059/1‑70 (MQ=255)

                            taacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg                                                      >  1:377381/3‑59 (MQ=255)

                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                           >  1:3386963/1‑70 (MQ=255)

                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                           >  1:2944604/1‑70 (MQ=255)

                            tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                           >  1:729627/1‑70 (MQ=255)

                              acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat                                        >  1:831528/1‑70 (MQ=255)

                              acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc                                        <  1:3891851/71‑1 (MQ=255)

                               caTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaatc                                       <  1:960083/71‑3 (MQ=255)

                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                       <  1:1891077/71‑1 (MQ=255)

                               caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                       <  1:573784/71‑1 (MQ=255)

                                 ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                     <  1:1018982/71‑1 (MQ=255)

                                 ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                     <  1:3657448/71‑1 (MQ=255)

                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                     >  1:331902/1‑70 (MQ=255)

                                  tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                     >  1:2753474/1‑70 (MQ=255)

                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa                                                        <  1:3172012/47‑1 (MQ=255)

                                      aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                              <  1:778934/57‑1 (MQ=255)

                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                              >  1:2829819/1‑56 (MQ=255)

                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg                                <  1:1832053/70‑1 (MQ=255)

                                       tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                               >  1:3470445/1‑71 (MQ=255)

                                        aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                              >  1:3243634/1‑71 (MQ=255)

                                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGtgc                                                                 >  1:1411956/1‑35 (MQ=255)

                                         aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                              <  1:62314/70‑1 (MQ=255)

                                              tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                        >  1:1873291/1‑71 (MQ=255)

                                               cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc                                              <  1:1589313/48‑1 (MQ=255)

                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                      >  1:1827212/1‑55 (MQ=255)

                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                       <  1:1738194/70‑1 (MQ=255)

                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                       <  1:1697944/70‑1 (MQ=255)

                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                      >  1:1389065/1‑71 (MQ=255)

                                                gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                      <  1:1414431/71‑1 (MQ=255)

                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                     <  1:2641752/71‑1 (MQ=255)

                                                 cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                     >  1:1063083/1‑71 (MQ=255)

                                                  ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                  <  1:280419/57‑1 (MQ=255)

                                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                   <  1:3518380/70‑1 (MQ=255)

                                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                    <  1:2901691/69‑1 (MQ=255)

                                                    cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                    <  1:1413438/69‑1 (MQ=255)

                                                       tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                 <  1:1517361/69‑1 (MQ=255)

                                                        gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata               <  1:2228124/70‑1 (MQ=255)

                                                         aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat             <  1:501691/71‑1 (MQ=255)

                                                         aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                >  1:3220389/1‑68 (MQ=255)

                                                          tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa              >  1:3037491/1‑69 (MQ=255)

                                                           aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                 >  1:1638895/1‑49 (MQ=255)

                                                               ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                      >  1:2593501/1‑40 (MQ=255)

                                                                cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa         >  1:1563804/1‑68 (MQ=255)

                                                                 aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga       >  1:1388102/1‑69 (MQ=255)

                                                                 aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga     <  1:3061942/71‑1 (MQ=255)

                                                                  gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc                  >  1:2859084/1‑57 (MQ=255)

                                                                    tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa  <  1:50266/71‑1 (MQ=255)

GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGA  >  minE/2595471‑2595609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A