Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,746 G→A 33.3% I284I (ATC→ATT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7460GA33.3% 135.6 / 99.1 120I284I (ATC→ATT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (41/39);  new base A (23/17);  total (64/56)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 131 total aligned reads displayed.

CCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGA  >  minE/2595678‑2595813
                                                                    |                                                                   
ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                                   <  1:328670/71‑1 (MQ=255)
ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                                   <  1:5096957/71‑1 (MQ=255)
 cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                                   >  1:4179180/1‑70 (MQ=255)
 cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                                   <  1:3390875/70‑1 (MQ=255)
  aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                                                 <  1:3853428/71‑1 (MQ=255)
      aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                             >  1:5290268/1‑71 (MQ=255)
          atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                                   >  1:4202109/1‑61 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                        <  1:5425102/71‑1 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                        <  1:2489466/71‑1 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:3432388/70‑1 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:639752/70‑1 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         >  1:4215380/1‑69 (MQ=255)
             ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                                      >  1:3361955/1‑71 (MQ=255)
             ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc                                                         >  1:1502745/1‑68 (MQ=255)
              cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                       <  1:2437817/69‑1 (MQ=255)
                 tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                  >  1:2040723/1‑71 (MQ=255)
                  aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                  <  1:5190734/70‑1 (MQ=255)
                  aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                  <  1:1520409/70‑1 (MQ=255)
                    tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                        <  1:4721886/62‑1 (MQ=255)
                    tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                 <  1:177354/69‑1 (MQ=255)
                    tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                 <  1:553044/69‑1 (MQ=255)
                    tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                 <  1:395201/69‑1 (MQ=255)
                      cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                                                           <  1:4706119/57‑1 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                            >  1:5226937/1‑54 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                            >  1:1661599/1‑54 (MQ=255)
                         cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                            >  1:4682640/1‑53 (MQ=255)
                         cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                            >  1:5413957/1‑53 (MQ=255)
                         cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                           <  1:4448805/70‑1 (MQ=255)
                         cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                           >  1:311521/1‑70 (MQ=255)
                         cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                           >  1:1359999/1‑70 (MQ=255)
                         cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                           >  1:5508891/1‑70 (MQ=255)
                           ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                                     <  1:5193117/42‑1 (MQ=255)
                           ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                        <  1:4337317/71‑1 (MQ=255)
                            tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                        >  1:2682276/1‑70 (MQ=255)
                            tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                        >  1:4959400/1‑70 (MQ=255)
                            tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:4538676/47‑1 (MQ=255)
                            tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:1414141/47‑1 (MQ=255)
                            tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:4774993/47‑1 (MQ=255)
                            tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:5031492/47‑1 (MQ=255)
                             gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                                             >  1:495566/1‑48 (MQ=255)
                             gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                            >  1:5028236/1‑49 (MQ=255)
                             gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                                   >  1:5300690/1‑57 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                                   >  1:4180699/1‑42 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                              >  1:1478264/1‑47 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                              >  1:5657266/1‑47 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                            >  1:838677/1‑49 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                            >  1:3537021/1‑49 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                      >  1:5474520/1‑71 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                      >  1:3144657/1‑71 (MQ=255)
                                cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                                   >  1:117966/1‑71 (MQ=255)
                                 aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                                   <  1:5676939/70‑1 (MQ=255)
                                   gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                                >  1:3936944/1‑71 (MQ=255)
                                    ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                                 >  1:3117455/1‑68 (MQ=255)
                                    ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                                 >  1:176605/1‑68 (MQ=255)
                                    ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:5257019/39‑1 (MQ=255)
                                    ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:4000200/39‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                     >  1:1163787/1‑48 (MQ=255)
                                      aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                     <  1:1097056/63‑1 (MQ=255)
                                      aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                     <  1:3387899/63‑1 (MQ=255)
                                      aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                     <  1:192852/63‑1 (MQ=255)
                                      aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                     <  1:4301593/63‑1 (MQ=255)
                                      aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg                             <  1:906572/71‑3 (MQ=255)
                                      aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:880141/43‑1 (MQ=255)
                                      aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:3889169/43‑1 (MQ=255)
                                      aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:3435103/43‑1 (MQ=255)
                                      aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                                      >  1:5338130/1‑46 (MQ=255)
                                       tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                  <  1:4049902/49‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                               <  1:1484990/36‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         <  1:3462397/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                              <  1:299542/69‑1 (MQ=255)
                                        aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                             >  1:2089697/1‑37 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                              >  1:1532783/1‑51 (MQ=255)
                                          accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt                         <  1:719264/71‑1 (MQ=255)
                                           ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         >  1:457586/1‑38 (MQ=255)
                                           ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         >  1:1349049/1‑38 (MQ=255)
                                           ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                         >  1:1025090/1‑38 (MQ=255)
                                            caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                       <  1:257140/71‑1 (MQ=255)
                                              ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc                     <  1:2879642/71‑1 (MQ=255)
                                               caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                    <  1:3722434/71‑1 (MQ=255)
                                                aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                        >  1:1036405/1‑50 (MQ=255)
                                                  aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                       >  1:2385229/1‑65 (MQ=255)
                                                  aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                   <  1:5510319/69‑1 (MQ=255)
                                                  aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt                 <  1:1668657/71‑1 (MQ=255)
                                                   tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt                 <  1:1845789/70‑1 (MQ=255)
                                                     cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                           >  1:4378702/1‑57 (MQ=255)
                                                     cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                           >  1:5689818/1‑57 (MQ=255)
                                                     cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc              >  1:180481/1‑71 (MQ=255)
                                                     cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc              >  1:1060550/1‑71 (MQ=255)
                                                      aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                     <  1:2724969/47‑1 (MQ=255)
                                                       tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                        <  1:2975736/59‑1 (MQ=255)
                                                       tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                  <  1:4282590/49‑1 (MQ=255)
                                                       tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                  <  1:4840439/49‑1 (MQ=255)
                                                        gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                      >  1:3895040/1‑44 (MQ=255)
                                                         ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg          <  1:2361138/71‑1 (MQ=255)
                                                          cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt         <  1:4160915/71‑1 (MQ=255)
                                                                agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACtt                                   >  1:3309099/1‑39 (MQ=19)
                                                                 gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt        >  1:189617/1‑65 (MQ=255)
                                                                 gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt        >  1:2317579/1‑65 (MQ=255)
                                                                 gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga  >  1:713254/1‑71 (MQ=255)
                                                                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc           >  1:1017289/1‑61 (MQ=255)
                                                                    |                                                                   
CCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGA  >  minE/2595678‑2595813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A