Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,919,366 A→G 21.9% G110G (GGT→GGC)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,919,3660AG21.9% 254.0 / 69.5 137G110G (GGT→GGC) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (58/49);  new base G (18/12);  total (76/61)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 143 total aligned reads displayed.

GAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCT  >  minE/2919297‑2919435
                                                                     |                                                                     
gAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGAc                                                                      >  1:1907166/1‑71 (MQ=255)
  aCTCATTGGTCAGGCCATTCCATACCTGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCa                                                                    <  1:58522/71‑1 (MQ=255)
   cTCGTTGGTCAGCCCGTTCCACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGccgt                                                                   <  1:2124962/71‑3 (MQ=255)
     cATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAg                                                                 >  1:1217205/1‑71 (MQ=255)
      aTTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGc                                                                >  1:5674693/1‑71 (MQ=255)
      aTTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGc                                                                >  1:5324878/1‑71 (MQ=255)
          gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                             <  1:4780463/70‑1 (MQ=255)
          gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                             <  1:2973416/70‑1 (MQ=255)
          gTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgc                                                             <  1:2879803/70‑1 (MQ=255)
           tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           <  1:3589598/71‑1 (MQ=255)
           tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           <  1:1858072/71‑1 (MQ=255)
             aGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGAt                                                          <  1:3962260/70‑1 (MQ=255)
              ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           <  1:1413791/68‑1 (MQ=255)
              ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           <  1:241723/68‑1 (MQ=255)
               gCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACt                                                       <  1:1812446/71‑1 (MQ=255)
                ccgttCCACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACt                                                       >  1:306238/4‑70 (MQ=255)
                ccgttCCACACCGGCACGCCCGCATACTGCGCCAGCGGTTCGACCACTTCCTGGCCGTGACCGCGATACTg                                                      <  1:4983946/68‑1 (MQ=255)
                ccATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTg                                                      <  1:1675769/71‑1 (MQ=255)
                 cATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGa                                                     <  1:4742506/71‑1 (MQ=255)
                  gttCCACACCGGCACGCCCGCATACTGCGCCAGCGTATCGACCACTTCCTGGCCGTGACCGCGATACTg                                                      <  1:1508771/68‑1 (MQ=255)
                   ttCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           <  1:3283864/63‑1 (MQ=255)
                     ccATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATAc                                                 >  1:402945/1‑71 (MQ=255)
                     ccATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATAc                                                 >  1:5014182/1‑71 (MQ=255)
                     ccACACCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAAtgc                                                 >  1:3211553/1‑69 (MQ=255)
                      cATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATa                                                  <  1:4792160/69‑1 (MQ=255)
                       aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgcg                                                            <  1:4121044/58‑1 (MQ=255)
                       aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCg                                               <  1:1697541/71‑1 (MQ=255)
                         aCCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGa                                                           >  1:4976226/1‑57 (MQ=255)
                         aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGt                                              >  1:733439/1‑70 (MQ=255)
                         aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTc                                             <  1:333869/71‑1 (MQ=255)
                         aCCGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTc                                             <  1:4298337/71‑1 (MQ=255)
                          ccGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTc                                             <  1:3940701/70‑1 (MQ=255)
                          ccGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                            >  1:4518782/1‑71 (MQ=255)
                          ccGGCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCa                                            >  1:2263157/1‑71 (MQ=255)
                            ggCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                          <  1:2234140/71‑1 (MQ=255)
                            ggCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                          >  1:4488857/1‑71 (MQ=255)
                             gCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATAc                                         <  1:3546077/71‑1 (MQ=255)
                             gCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                          >  1:269145/1‑70 (MQ=255)
                             gCACGCCCGCATACTGCGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATa                                          >  1:484841/1‑70 (MQ=255)
                              cACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                        >  1:10161/1‑71 (MQ=255)
                                 gCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATa                                                         >  1:3112073/1‑51 (MQ=255)
                                  cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGc                                     >  1:400834/1‑70 (MQ=255)
                                  cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCg                                    <  1:4496304/71‑1 (MQ=255)
                                  cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCg                                    <  1:5055038/71‑1 (MQ=255)
                                     gCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                   <  1:5155006/69‑1 (MQ=255)
                                       gTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGAcc                                 >  1:4695523/1‑69 (MQ=255)
                                       gTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGAcc                                 >  1:4983560/1‑69 (MQ=255)
                                        tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCcgcg                                                            >  1:159110/1‑41 (MQ=255)
                                        tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                              >  1:4119429/1‑71 (MQ=255)
                                        tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                              >  1:2112056/1‑71 (MQ=255)
                                        tATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATAATGAATACCGTCATACATGCGACCAAg                              >  1:5192670/1‑71 (MQ=255)
                                         aTTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                              >  1:1579204/1‑70 (MQ=255)
                                         aTTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                              >  1:776690/1‑70 (MQ=255)
                                          ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGaa                                                    >  1:1573367/1‑47 (MQ=255)
                                          ttCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                            >  1:2796713/1‑55 (MQ=255)
                                           tCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:5674224/1‑70 (MQ=255)
                                            ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                   >  1:3163553/1‑62 (MQ=255)
                                            ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcg                          >  1:832260/1‑71 (MQ=255)
                                            ccGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                           <  1:949554/70‑1 (MQ=255)
                                             cGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                        >  1:975375/1‑56 (MQ=255)
                                             cGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACa                                        >  1:4034677/1‑56 (MQ=255)
                                             cGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcgc                         <  1:4914136/71‑1 (MQ=255)
                                             cGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTTAATGCCGTCATACATCCGCCCGAGAAc                           <  1:383035/69‑1 (MQ=255)
                                             cGCCAGCGTTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAAc                           <  1:1889663/69‑1 (MQ=255)
                                              gCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGt                                              <  1:305113/49‑1 (MQ=255)
                                              gCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGt                                              <  1:2428923/49‑1 (MQ=255)
                                                cAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                            >  1:550620/1‑49 (MQ=255)
                                                cAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCa                                            >  1:1321866/1‑49 (MQ=255)
                                                 agcgtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCgcgc                     <  1:2994542/68‑1 (MQ=255)
                                                  gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                      >  1:3615736/1‑69 (MQ=255)
                                                  gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                      >  1:846323/1‑69 (MQ=255)
                                                  gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                      >  1:1392164/1‑69 (MQ=255)
                                                  gtgtTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                      >  1:1101333/1‑69 (MQ=255)
                                                   cgtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAGAACCCgcgcgg                   >  1:1599345/2‑69 (MQ=255)
                                                    gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACAtc                                      >  1:4166330/1‑50 (MQ=255)
                                                    gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACAtc                                      >  1:1706309/1‑50 (MQ=255)
                                                    gtTTCGACCACTTCCTGGCCGTGACCGCGATACTGAATGCCGTCATACATCCGCCCGAgaa                            >  1:2348327/1‑59 (MQ=255)
                                                     tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGa                                   <  1:3460941/53‑1 (MQ=255)
                                                     tttCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgt                  <  1:2736729/70‑1 (MQ=255)
                                                     tttCGACAATCTCCTGACAATAGCCGCGATACTGAATACCGTCATAc                                         <  1:1045940/47‑1 (MQ=255)
                                                       tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:137224/1‑58 (MQ=255)
                                                       tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTc               >  1:4688723/1‑71 (MQ=255)
                                                       tCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTc               >  1:161096/1‑71 (MQ=255)
                                                        cGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:3830768/1‑57 (MQ=255)
                                                         gACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt             <  1:5474177/71‑1 (MQ=255)
                                                         gACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt             <  1:2301822/71‑1 (MQ=255)
                                                           cAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:5171538/1‑54 (MQ=255)
                                                            aaTCTCCTGACCATAGCCGCGGTACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTaa          >  1:1903331/1‑71 (MQ=255)
                                                            aaTCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcgc                         >  1:2663031/1‑56 (MQ=255)
                                                                  cTGACCATAGCCGCTATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAct    >  1:4619687/1‑71 (MQ=255)
                                                                  cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATc        <  1:1424075/67‑1 (MQ=255)
                                                                  cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGa      >  1:2821532/1‑69 (MQ=255)
                                                                  cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAct    >  1:2343272/1‑71 (MQ=255)
                                                                  cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGAGTCTTTAATCGAct    >  1:772278/1‑71 (MQ=255)
                                                                   tGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGa      <  1:629304/68‑1 (MQ=255)
                                                                   tGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGActc   >  1:5559379/1‑71 (MQ=255)
                                                                    gACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTAAATCGActct  >  1:2658552/1‑71 (MQ=255)
                                                                     aCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:1658580/1‑44 (MQ=255)
                                                                     aCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                            >  1:4250198/1‑44 (MQ=255)
                                                                     aCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgtgt                >  1:2735544/1‑56 (MQ=255)
                                                                     |                                                                     
GAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCT  >  minE/2919297‑2919435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A