Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I2 R1	721	0.0	1472758	54.8%	807071	56.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	395,569	A→G	intergenic (‑58/‑294)	ampH ← / → sbmA	beta‑lactamase/D‑alanine carboxypeptidase/predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	395,569	0	A	G	100.0%	172.5 / NA	61	intergenic (‑58/‑294)	ampH/sbmA	beta‑lactamase/D‑alanine carboxypeptidase/predicted transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/0); total (61/0)

TCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATAGATTGCAAAGGGCGAATTAGTGCAATA  >  W3110S.gb/395508‑395596

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1082298/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:860188/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1122632/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1130220/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:780292/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1144279/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1059980/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:224938/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:170644/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1362219/62‑1 (MQ=255)

tCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:1264608/62‑1 (MQ=255)

                        atgaaGGTAACTGAGCGTATTTATACTACTCATTGATa                             <  1:315123/38‑1 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCaa                     >  1:1181585/1‑42 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGc      >  1:261275/1‑57 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaata  >  1:917232/1‑61 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:795200/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:404227/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:434312/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:452432/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:528250/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:534456/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:536614/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:64488/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:65087/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:680018/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:740898/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:746062/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:758310/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1143011/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:794297/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:389907/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:818176/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:841837/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:846117/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:85216/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1106215/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:876869/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:898443/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:907300/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:946128/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:94822/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:958946/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:966936/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:171998/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1240273/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1282956/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1287218/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1317725/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1338928/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1236528/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1364164/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1406444/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:145311/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1463339/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1470499/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:15206/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:164556/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1228914/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1243139/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:17287/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:197809/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:205260/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:210239/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1211016/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:236807/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:1206990/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:114318/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:318480/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:352226/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:363990/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:370924/1‑60 (MQ=255)

                            aGGTAACTGAGCGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:375587/1‑60 (MQ=255)

                            aGGTAACTGAGAGTATTTATACTACTCATTGATGGATTGCAAAGGGCGAATTAGTGCaat   >  1:669225/1‑60 (MQ=255)

TCAACGGTGGTGTCCATAGATTGAATGAAGGTAACTGAGCGTATTTATACTACTCATTGATAGATTGCAAAGGGCGAATTAGTGCAATA  >  W3110S.gb/395508‑395596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A