Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
926,386 |
Δ2 bp |
noncoding (7‑8/88 nt) |
serW ← |
tRNA‑Ser |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 926,384 | 0 | A | . | 100.0%
| 63.2
/ NA
| 17 | noncoding (10/88 nt) | serW | tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (0/17); total (0/17) |
| * | W3110S.gb | 926,385 | 0 | C | . | 100.0%
| 63.2
/ NA
| 17 | noncoding (9/88 nt) | serW | tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (0/17); total (0/17) |
CCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGG > W3110S.gb/926369‑926431
||
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:577280/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:808787/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:779579/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:685370/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:676852/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:639772/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:618663/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:591109/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:58224/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:1031386/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:525059/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:417151/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:155536/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:138220/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:1337456/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:116911/61‑1 (MQ=38)
ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg < 1:1099307/61‑1 (MQ=38)
||
CCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGG > W3110S.gb/926369‑926431
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
