Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,261,172 |
C→T |
A403S (GCG→TCA) |
ychM ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,261,172 | 0 | C | T | 85.0%
| 111.2
/ 9.5
| 60 | A403S (GCG→TCA) | ychM | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (9/0); new base T (51/0); total (60/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCA > W3110S.gb/1261137‑1261208
|
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATg > 1:118570/1‑41 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATg > 1:847607/1‑41 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATg > 1:61559/1‑41 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:721668/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:378889/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:385279/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:385418/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:394917/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:400067/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:405050/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:430940/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:539168/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:610349/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:625777/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:639850/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:707480/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1161676/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:725091/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:731748/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:734496/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:735049/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:74097/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:75647/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:757132/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:76235/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:795950/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:881063/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:912669/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:33368/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1208634/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1217988/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1227269/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1243986/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1266168/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:127038/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1298319/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:131867/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1328954/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1329951/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1345556/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1349741/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1364517/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1416179/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:1417105/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:145060/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:187614/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:196923/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:235664/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:270940/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:272698/1‑42 (MQ=38)
aTGCACAGCAGCATGACGATGATGTCATCTTTCGGTGAATGa > 1:289065/1‑42 (MQ=38)
gcaTGACGATGATGTCATCTTTCGTCTCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCa > 1:35920/1‑62 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGCCCACTTTGTGCGCTTc > 1:287938/1‑61 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGCCCACTTTGTGCGCTTCa > 1:803505/1‑62 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCAc > 1:491245/1‑49 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTcc > 1:114547/1‑61 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTc > 1:169210/1‑61 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCa > 1:919424/1‑62 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCa > 1:625412/1‑62 (MQ=255)
gcaTGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCa > 1:700974/1‑62 (MQ=255)
|
ATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCA > W3110S.gb/1261137‑1261208
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
