Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,390,940 C→G A99A (GCC→GCG)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,390,9400CG100.0% 354.9 / NA 125A99A (GCC→GCG) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (0/125);  total (0/125)

Only 100 of 125 total aligned reads displayed.

GCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAC  >  W3110S.gb/1390923‑1390984
                 |                                            
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGCTCGGCATAAc  <  1:714835/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGTCATAAc  <  1:495775/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATaag  <  1:1051876/61‑2 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:514915/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:68506/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:678020/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:677634/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:633954/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:632444/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:623417/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:617029/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:568403/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:56569/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:559230/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:54812/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:516150/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:391062/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:514697/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:510595/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:487754/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:484737/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:468745/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:456071/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:454535/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:447250/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:436651/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:36823/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:828789/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:939098/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:921318/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:909667/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:907056/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:897672/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:888720/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:8858/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:885625/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:875328/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:843754/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:832784/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:699159/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:813023/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:802515/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:795376/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:791209/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:779475/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:777737/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:771551/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:771280/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:75877/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:757144/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:748608/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1235109/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1356270/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1333074/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1327792/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1322729/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1320044/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1319749/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1293346/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1273968/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1254714/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1250326/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1237948/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1393132/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1233375/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1232054/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1199040/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1183770/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1176527/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1115486/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1094046/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1085049/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1076825/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1053559/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1047028/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:222905/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:340774/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:333203/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:320212/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:318316/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:312462/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:303074/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:280662/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:279413/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:256246/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:255519/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:241931/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1015918/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:209657/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:207863/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:18403/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:148545/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1467614/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1452252/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1437449/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:14299/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1420457/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1419566/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc  <  1:1396831/61‑1 (MQ=255)
 cATTGTGGGATCTGGCGGCGCGAAAACAGCAGCAATCGCTGCCTGATTTGATCGGCATAAc  <  1:1100636/61‑1 (MQ=38)
                 |                                            
GCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAC  >  W3110S.gb/1390923‑1390984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A