Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,293,655 |
T→C |
intergenic (‑240/‑179) |
insH ← / → narP |
IS5 transposase and trans‑activator/DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,293,655 | 0 | T | C | 96.7%
| 75.3
/ ‑6.4
| 30 | intergenic (‑240/‑179) | insH/narP | IS5 transposase and trans‑activator/DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (29/0); minor base A (1/0); total (30/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAA > W3110S.gb/2293604‑2293664
|
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:325001/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:96915/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:957306/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:824839/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:778179/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:776424/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:763992/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:667767/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:631221/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:613159/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:507126/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:489192/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:468428/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:361227/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1020940/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:235780/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1441955/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1439648/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1397757/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1373637/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1329440/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1263146/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1179650/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1128632/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1102561/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1080924/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1055699/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCtg > 1:1047635/1‑54 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCCTGATgagat > 1:890236/1‑60 (MQ=255)
aCTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCAtg > 1:1151687/1‑54 (MQ=255)
|
ACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTTCTTGATGAGAA > W3110S.gb/2293604‑2293664
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
